View source for OMP ST:73900 ! Escherichia coli K-12 JW4172

__TOC__

==Strain Summary==
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{|   id="B5b510879e52d3"  class=" tableEdit Strain_info_table"  

|-
!align=left align='left' bgcolor='#CCCCFF' |Strain Name
||
Escherichia coli K-12 JW4172
|-
!align=left align='left' bgcolor='#CCCCFF' |Synonyms
||
JW4172-1
|-
!align=left align='left' bgcolor='#CCCCFF' |Taxon Information
||
*Pangenome: Escherichia coli
*Subspecies and/or strain: K-12
*NCBI Taxonomy ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
|-
!align=left align='left' bgcolor='#CCCCFF' |Genotype
||
DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 cysQ763(del)::FRT-kan-FRT
|-
!align=left align='left' bgcolor='#CCCCFF' |Strain Reference
||
*PMID:16738554
*[http://cgsc2.biology.yale.edu/Strain.php?ID=109286 Coli Genetic Stock Center]
|-
!align=left align='left' bgcolor='#CCCCFF' |Strain availability
||
[http://cgsc2.biology.yale.edu/Strain.php?ID=109286 Coli Genetic Stock Center]
|-
!align=left align='left' bgcolor='#CCCCFF' |Ancestry
||
parent: [[OMP ST:800 ! Escherichia coli K-12 BW25113]]
|-
!align=left align='left' bgcolor='#CCCCFF' |Annotated phenotypes
||


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==Notes==


==Phenotype Annotation==
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!|Annotation ID!!Qualifier!!OMP ID!!OMP term name!!Relative phenotype information!!Experimental condition!!ECO ID!!ECO term name!!Reference!!Annotation Extension!!Notes
|- 
|
OMP_AN:35275
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27601

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysQ763(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Sodium Acetate anhydrous
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:32954) Sodium Acetate anhydrous
|

*chemical concentration= 0.6%
*normalized fitness= -7.76 
|- 
|
OMP_AN:35276
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27611

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysQ763(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Sodium Acetate anhydrous
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:32954) Sodium Acetate anhydrous
|

*chemical concentration= 0.6%
*normalized fitness= -7.76 
|- 
|
OMP_AN:35277
|

|
OMP:0007927
|
decreased anaerobic population growth
|
Relative to: OMP_AN:27618

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysQ763(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Anaerobic
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|

|

*
*normalized fitness= -3.63 
|- 
|
OMP_AN:35278
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27606

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysQ763(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:N-Acetyl-D-glucosamine
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:506227) N-Acetyl-D-glucosamine
|

*chemical concentration= 0.15%
*normalized fitness= -13.07 
|- 
|
OMP_AN:35279
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27616

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysQ763(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:N-Acetyl-D-glucosamine
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:506227) N-Acetyl-D-glucosamine
|

*chemical concentration= 0.15%
*normalized fitness= -13.07 
|- 
|
OMP_AN:35280
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27602

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysQ763(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-(+) Glucosamine hydrochloride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:91431) D-(+) Glucosamine hydrochloride
|

*chemical concentration= 0.2%
*normalized fitness= -10.03 
|- 
|
OMP_AN:35281
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27612

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysQ763(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-(+) Glucosamine hydrochloride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:91431) D-(+) Glucosamine hydrochloride
|

*chemical concentration= 0.2%
*normalized fitness= -10.03 
|- 
|
OMP_AN:35282
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27603

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysQ763(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-Glucose anhydrous
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:42758) D-Glucose anhydrous
|

*chemical concentration= 0.2%
*normalized fitness= -14.18 
|- 
|
OMP_AN:35283
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27613

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysQ763(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-Glucose anhydrous
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:42758) D-Glucose anhydrous
|

*chemical concentration= 0.2%
*normalized fitness= -14.18 
|- 
|
OMP_AN:35284
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27604

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysQ763(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Glycerol
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:17754) Glycerol
|

*chemical concentration= 0.4%
*normalized fitness= -6.65 
|- 
|
OMP_AN:35285
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27614

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysQ763(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Glycerol
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:17754) Glycerol
|

*chemical concentration= 0.4%
*normalized fitness= -6.65 
|- 
|
OMP_AN:35286
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27605

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysQ763(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-(+) Maltose monohydrate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:23615261) D-(+) Maltose monohydrate
|

*chemical concentration= 0.1%
*normalized fitness= -13.1 
|- 
|
OMP_AN:35287
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27615

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysQ763(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-(+) Maltose monohydrate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:23615261) D-(+) Maltose monohydrate
|

*chemical concentration= 0.1%
*normalized fitness= -13.1 
|- 
|
OMP_AN:35288
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27600

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysQ763(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:NH4Cl
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:31206) NH4Cl
|

*chemical concentration= 9.5mm
*normalized fitness= -5.89 
|- 
|
OMP_AN:35289
|

|
OMP:0007032
|
decreased utilization of inorganic nitrogen source
|
Relative to: OMP_AN:27610

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysQ763(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:NH4Cl
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:31206) NH4Cl
|

*chemical concentration= 9.5mm
*normalized fitness= -5.89 
|- 
|
OMP_AN:35290
|

|
OMP:0007878
|
increased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27547

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysQ763(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Hydrogen peroxide
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:16240) Hydrogen peroxide
|

*chemical concentration=  2 mM
*normalized fitness= 4.25 
|- 
|
OMP_AN:35291
|

|
OMP:0007249
|
decreased resistance to a chemical
|
Relative to: OMP_AN:27573

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysQ763(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Procaine hydrochloride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:8431) Procaine hydrochloride
|

*chemical concentration=  10 mM
*normalized fitness= -4.01 
|- 
|
OMP_AN:35292
|

|
OMP:0007896
|
decreased resistance to a surfactant
|
Relative to: OMP_AN:27582

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysQ763(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:SDS
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:8984) SDS
|

*chemical concentration= 0.5 %
*normalized fitness= -3.49 
|- 
|
OMP_AN:35293
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27607

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysQ763(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Succinic acid
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:15741) Succinic acid
|

*chemical concentration= 0.3%
*normalized fitness= -8.87 
|- 
|
OMP_AN:35294
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27617

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysQ763(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Succinic acid
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:15741) Succinic acid
|

*chemical concentration= 0.3%
*normalized fitness= -8.87 
|- 
|
OMP_AN:35295
|

|
OMP:0007878
|
increased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27516

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysQ763(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Thiolactomycin
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:445629) Thiolactomycin
|

*chemical concentration=  5 uM
*normalized fitness= 6.08 
|- 
|
OMP_AN:35296
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27598

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysQ763(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron excess-FeSO4 heptahydride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride
|

*chemical concentration= 1mM (MOPS; normal 100uM)
*normalized fitness= -6.02 
|- 
|
OMP_AN:35297
|

|
OMP:0007886
|
decreased resistance to a metal
|
Relative to: OMP_AN:27608

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysQ763(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron excess-FeSO4 heptahydride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride
|

*chemical concentration= 1mM (MOPS; normal 100uM)
*normalized fitness= -6.02 
|- 
|
OMP_AN:35298
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27599

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysQ763(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron starvation-FeSO4·7H2O
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O
|

*chemical concentration= 2uM (MOPS; normal 100uM)
*normalized fitness= -6.63 
|- 
|
OMP_AN:35299
|

|
OMP:0007937
|
decreased resistance to nutrient limitation
|
Relative to: OMP_AN:27609

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysQ763(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron starvation-FeSO4·7H2O
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O
|

*chemical concentration= 2uM (MOPS; normal 100uM)
*normalized fitness= -6.63 
|- 
|
OMP_AN:35300
|

|
OMP:0007878
|
increased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27551

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysQ763(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Rifampicin
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:28077) Rifampicin
|

*chemical concentration=  2 ug/ml
*normalized fitness= 3.92 

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==Additional Information==


==Links==


==References==
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