View source for OMP ST:73875 ! Escherichia coli K-12 JW4135

__TOC__

==Strain Summary==
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!align=left align='left' bgcolor='#CCCCFF' |Strain Name
||
Escherichia coli K-12 JW4135
|-
!align=left align='left' bgcolor='#CCCCFF' |Synonyms
||
JW4135-2
|-
!align=left align='left' bgcolor='#CCCCFF' |Taxon Information
||
*Pangenome: Escherichia coli
*Subspecies and/or strain: K-12
*NCBI Taxonomy ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
|-
!align=left align='left' bgcolor='#CCCCFF' |Genotype
||
DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 purA727(del)::FRT-kan-FRT
|-
!align=left align='left' bgcolor='#CCCCFF' |Strain Reference
||
*PMID:16738554
*[http://cgsc2.biology.yale.edu/Strain.php?ID=109259 Coli Genetic Stock Center]
|-
!align=left align='left' bgcolor='#CCCCFF' |Strain availability
||
[http://cgsc2.biology.yale.edu/Strain.php?ID=109259 Coli Genetic Stock Center]
|-
!align=left align='left' bgcolor='#CCCCFF' |Ancestry
||
parent: [[OMP ST:800 ! Escherichia coli K-12 BW25113]]
|-
!align=left align='left' bgcolor='#CCCCFF' |Annotated phenotypes
||


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==Notes==


==Phenotype Annotation==
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="H5b510871d1dae"  class=" tableEdit OMP_annotation_table dataTable"  
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!|Annotation ID!!Qualifier!!OMP ID!!OMP term name!!Relative phenotype information!!Experimental condition!!ECO ID!!ECO term name!!Reference!!Annotation Extension!!Notes
|- 
|
OMP_AN:35177
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27601

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Sodium Acetate anhydrous
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:32954) Sodium Acetate anhydrous
|

*chemical concentration= 0.6%
*normalized fitness= -8.28 
|- 
|
OMP_AN:35178
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27611

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Sodium Acetate anhydrous
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:32954) Sodium Acetate anhydrous
|

*chemical concentration= 0.6%
*normalized fitness= -8.28 
|- 
|
OMP_AN:35179
|

|
OMP:0006058
|
increased resistance to aminoglycoside
|
Relative to: OMP_AN:27525

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Amikacin sulfate salt (disulfate)
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:2638) Amikacin sulfate salt (disulfate)
|

*chemical concentration= 0.05 ug/ml
*normalized fitness= 3.75 
|- 
|
OMP_AN:35180
|

|
OMP:0006037
|
increased resistance to macrolide
|
Relative to: OMP_AN:27518

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Azithromycin
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:2955) Azithromycin
|

*chemical concentration= 0.02 ug/ml
*normalized fitness= 4.35 
|- 
|
OMP_AN:35181
|

|
OMP:0007895
|
increased resistance to a surfactant
|
Relative to: OMP_AN:27581

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Benzalkonium chloride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:3020) Benzalkonium chloride
|

*chemical concentration= 1 ug/ml
*normalized fitness= 3.89 
|- 
|
OMP_AN:35182
|

|
OMP:0007903
|
decreased resistance to bile acids
|
Relative to: OMP_AN:27587

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:26711 + CHEBI:9177) Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)
|

*chemical concentration= 0.1 %
*normalized fitness= -5.05 
|- 
|
OMP_AN:35183
|

|
OMP:0007249
|
decreased resistance to a chemical
|
Relative to: OMP_AN:27514

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Chlorpromazine hydrochloride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:3649) Chlorpromazine hydrochloride
|

*chemical concentration=  6 uM
*normalized fitness= -3.87 
|- 
|
OMP_AN:35184
|

|
OMP:0007886
|
decreased resistance to a metal
|
Relative to: OMP_AN:27579

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Copper stress-CuCl2 dihydrate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:86318) Copper stress-CuCl2 dihydrate
|

*chemical concentration=  4 mM (LB)
*normalized fitness= -3.42 
|- 
|
OMP_AN:35185
|

|
OMP:0007903
|
decreased resistance to bile acids
|
Relative to: OMP_AN:27586

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Deoxycholate sodium
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:9177) Deoxycholate sodium
|

*chemical concentration=  2 %
*normalized fitness= -3.2 
|- 
|
OMP_AN:35186
|

|
OMP:0007912
|
decreased resistance to a chelator
|
Relative to: OMP_AN:27507

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:EDTA disodium salt dihydrate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:64758) EDTA disodium salt dihydrate
|

*chemical concentration= 0.1 mM
*normalized fitness= -3.3 
|- 
|
OMP_AN:35187
|

|
OMP:0007249
|
decreased resistance to a chemical
|
Relative to: OMP_AN:27572

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Epigallocatechin gallate (EGCG)
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:4806) Epigallocatechin gallate (EGCG)
|

*chemical concentration=  20 uM
*normalized fitness= -3.62 
|- 
|
OMP_AN:35188
|

|
OMP:0006036
|
decreased resistance to macrolide
|
Relative to: OMP_AN:27520

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Erythromycin
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:42355) Erythromycin
|

*chemical concentration=  1 ug/ml
*normalized fitness= -4.13 
|- 
|
OMP_AN:35189
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27606

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:N-Acetyl-D-glucosamine
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:506227) N-Acetyl-D-glucosamine
|

*chemical concentration= 0.15%
*normalized fitness= -8.17 
|- 
|
OMP_AN:35190
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27616

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:N-Acetyl-D-glucosamine
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:506227) N-Acetyl-D-glucosamine
|

*chemical concentration= 0.15%
*normalized fitness= -8.17 
|- 
|
OMP_AN:35191
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27602

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-(+) Glucosamine hydrochloride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:91431) D-(+) Glucosamine hydrochloride
|

*chemical concentration= 0.2%
*normalized fitness= -13.67 
|- 
|
OMP_AN:35192
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27612

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-(+) Glucosamine hydrochloride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:91431) D-(+) Glucosamine hydrochloride
|

*chemical concentration= 0.2%
*normalized fitness= -13.67 
|- 
|
OMP_AN:35193
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27603

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-Glucose anhydrous
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:42758) D-Glucose anhydrous
|

*chemical concentration= 0.2%
*normalized fitness= -8.21 
|- 
|
OMP_AN:35194
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27613

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-Glucose anhydrous
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:42758) D-Glucose anhydrous
|

*chemical concentration= 0.2%
*normalized fitness= -8.21 
|- 
|
OMP_AN:35195
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27604

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Glycerol
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:17754) Glycerol
|

*chemical concentration= 0.4%
*normalized fitness= -8.92 
|- 
|
OMP_AN:35196
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27614

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Glycerol
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:17754) Glycerol
|

*chemical concentration= 0.4%
*normalized fitness= -8.92 
|- 
|
OMP_AN:35197
|

|
OMP:0007878
|
increased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27545

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Isoniazid
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:6030) Isoniazid
|

*chemical concentration= 0.2 mM
*normalized fitness= 3.91 
|- 
|
OMP_AN:35198
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27605

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-(+) Maltose monohydrate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:23615261) D-(+) Maltose monohydrate
|

*chemical concentration= 0.1%
*normalized fitness= -6.84 
|- 
|
OMP_AN:35199
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27615

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-(+) Maltose monohydrate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:23615261) D-(+) Maltose monohydrate
|

*chemical concentration= 0.1%
*normalized fitness= -6.84 
|- 
|
OMP_AN:35200
|

|
OMP:0006044
|
decreased resistance to beta-lactam
|
Relative to: OMP_AN:27537

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Mecillinam
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:51208) Mecillinam
|

*chemical concentration=  0.09 ug/ml
*normalized fitness= -3 
|- 
|
OMP_AN:35201
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27600

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:NH4Cl
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:31206) NH4Cl
|

*chemical concentration= 9.5mm
*normalized fitness= -11.51 
|- 
|
OMP_AN:35202
|

|
OMP:0007032
|
decreased utilization of inorganic nitrogen source
|
Relative to: OMP_AN:27610

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:NH4Cl
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:31206) NH4Cl
|

*chemical concentration= 9.5mm
*normalized fitness= -11.51 
|- 
|
OMP_AN:35203
|

|
OMP:0007878
|
increased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27503

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Nitrofurantoin
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:71415) Nitrofurantoin
|

*chemical concentration=  2 ug/ml
*normalized fitness= 3.71 
|- 
|
OMP_AN:35204
|

|
OMP:0007250
|
increased resistance to a chemical
|
Relative to: OMP_AN:27593

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:(-)-Norepinephrine
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:18357) (-)-Norepinephrine
|

*chemical concentration= 100 uM
*normalized fitness= 3.72 
|- 
|
OMP_AN:35205
|

|
OMP:0007249
|
decreased resistance to a chemical
|
Relative to: OMP_AN:27573

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Procaine hydrochloride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:8431) Procaine hydrochloride
|

*chemical concentration= 1 mM
*normalized fitness= -5.07 
|- 
|
OMP_AN:35206
|

|
OMP:0007878
|
increased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27553

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Puromycin dihydrochloride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:8642) Puromycin dihydrochloride
|

*chemical concentration=  25 ug/ml
*normalized fitness= 3.69 
|- 
|
OMP_AN:35207
|

|
OMP:0006041
|
increased resistance to quinolone
|
Relative to: OMP_AN:27543

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Streptonigrin
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:9287) Streptonigrin
|

*chemical concentration=  0.2 ug/ml
*normalized fitness= 3.99 
|- 
|
OMP_AN:35208
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27607

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Succinic acid
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:15741) Succinic acid
|

*chemical concentration= 0.3%
*normalized fitness= -4.87 
|- 
|
OMP_AN:35209
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27617

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Succinic acid
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:15741) Succinic acid
|

*chemical concentration= 0.3%
*normalized fitness= -4.87 
|- 
|
OMP_AN:35210
|

|
OMP:0007878
|
increased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27516

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Thiolactomycin
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:445629) Thiolactomycin
|

*chemical concentration=  5 uM
*normalized fitness= 3.55 
|- 
|
OMP_AN:35211
|

|
OMP:0006057
|
decreased resistance to aminoglycoside
|
Relative to: OMP_AN:27529

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Tobramycin
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:28864) Tobramycin
|

*chemical concentration=  0.1 ug/ml
*normalized fitness= -3.74 
|- 
|
OMP_AN:35212
|

|
OMP:0007879
|
decreased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27566

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Vancomycin HCl hydrate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:9932) Vancomycin HCl hydrate
|

*chemical concentration=  50 ug/ml
*normalized fitness= -3.37 
|- 
|
OMP_AN:35213
|

|
OMP:0007249
|
decreased resistance to a chemical
|
Relative to: OMP_AN:27568

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:(±)-Verapamil hydrochloride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:53188) (±)-Verapamil hydrochloride
|

*chemical concentration=  0.5 mM
*normalized fitness= -3.81 
|- 
|
OMP_AN:35214
|

|
OMP:0007879
|
decreased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27560

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Bacitracin Zinc Sulfate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:16219032) Bacitracin Zinc Sulfate
|

*chemical concentration=  300 ug/ml
*normalized fitness= -5.7 
|- 
|
OMP_AN:35215
|

|
OMP:0007912
|
decreased resistance to a chelator
|
Relative to: OMP_AN:27508

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:EGTA
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:30740) EGTA
|

*chemical concentration=   1 mM
*normalized fitness= -3.37 
|- 
|
OMP_AN:35216
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27598

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron excess-FeSO4 heptahydride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride
|

*chemical concentration= 1mM (MOPS; normal 100uM)
*normalized fitness= -8.38 
|- 
|
OMP_AN:35217
|

|
OMP:0007886
|
decreased resistance to a metal
|
Relative to: OMP_AN:27608

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron excess-FeSO4 heptahydride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride
|

*chemical concentration= 1mM (MOPS; normal 100uM)
*normalized fitness= -8.38 
|- 
|
OMP_AN:35218
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27599

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron starvation-FeSO4·7H2O
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O
|

*chemical concentration= 2uM (MOPS; normal 100uM)
*normalized fitness= -7.6 
|- 
|
OMP_AN:35219
|

|
OMP:0007937
|
decreased resistance to nutrient limitation
|
Relative to: OMP_AN:27609

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron starvation-FeSO4·7H2O
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O
|

*chemical concentration= 2uM (MOPS; normal 100uM)
*normalized fitness= -7.6 
|- 
|
OMP_AN:35220
|

|
OMP:0007521
|
decreased population growth in high osmolarity
|
Relative to: OMP_AN:27595

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:NaCl
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:26710) NaCl
|

*chemical concentration=  300 mM
*normalized fitness= -3.99 
|- 
|
OMP_AN:35221
|

|
OMP:0006045
|
increased resistance to beta-lactam compound
|
Relative to: OMP_AN:27538

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Oxacillin sodium salt
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:52134) Oxacillin sodium salt
|

*chemical concentration= 0.5 ug/ml
*normalized fitness= 3.9 
|- 
|
OMP_AN:35222
|

|
OMP:0007878
|
increased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27565

<hr>Genotype differences:<br>
+lacZ4787(del)( +purA727(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Polymyxin B sulfate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:8310) Polymyxin B sulfate
|

*chemical concentration=  2 ug/ml
*normalized fitness= 3.84 

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==Additional Information==


==Links==


==References==
{{RefHelp}}<references/>