View source for OMP ST:73766 ! Escherichia coli K-12 JW3993

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==Strain Summary==
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!align=left align='left' bgcolor='#CCCCFF' |Strain Name
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Escherichia coli K-12 JW3993
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!align=left align='left' bgcolor='#CCCCFF' |Synonyms
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JW3993-1
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!align=left align='left' bgcolor='#CCCCFF' |Taxon Information
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*Pangenome: Escherichia coli
*Subspecies and/or strain: K-12
*NCBI Taxonomy ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
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!align=left align='left' bgcolor='#CCCCFF' |Genotype
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DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 malF729(del)::FRT-kan-FRT
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!align=left align='left' bgcolor='#CCCCFF' |Strain Reference
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*PMID:16738554
*[http://cgsc2.biology.yale.edu/Strain.php?ID=109155 Coli Genetic Stock Center]
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!align=left align='left' bgcolor='#CCCCFF' |Strain availability
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[http://cgsc2.biology.yale.edu/Strain.php?ID=109155 Coli Genetic Stock Center]
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!align=left align='left' bgcolor='#CCCCFF' |Ancestry
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parent: [[OMP ST:800 ! Escherichia coli K-12 BW25113]]
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!align=left align='left' bgcolor='#CCCCFF' |Annotated phenotypes
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==Notes==


==Phenotype Annotation==
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!|Annotation ID!!Qualifier!!OMP ID!!OMP term name!!Relative phenotype information!!Experimental condition!!ECO ID!!ECO term name!!Reference!!Annotation Extension!!Notes
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OMP_AN:34994
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OMP:0007249
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decreased resistance to a chemical
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Relative to: OMP_AN:27594

<hr>Genotype differences:<br>
+lacZ4787(del)( +malF729(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:SDS (CHEBI:8984) +EDTA (CHEBI:64758)
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
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PMID:21185072
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towards some (CHEBI:64758 + CHEBI:8984) SDS (CHEBI:8984) +EDTA (CHEBI:64758)
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*chemical concentration=  0.5%/0.5mM %/0.5mM
*normalized fitness= -4.06 
|- 
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OMP_AN:34995
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OMP:0007878
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increased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27559

<hr>Genotype differences:<br>
+lacZ4787(del)( +malF729(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Actinomycin D
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
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PMID:21185072
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towards some (CHEBI:27666 CID:457193) Actinomycin D
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*chemical concentration=  15 ug/ml
*normalized fitness= 3.98 
|- 
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OMP_AN:34996
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OMP:0007879
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decreased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27549

<hr>Genotype differences:<br>
+lacZ4787(del)( +malF729(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:CHIR-090
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
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PMID:21185072
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towards some (CHEBI:134107 CID:11546620) CHIR-090
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*chemical concentration=  0.025 ug/ml
*normalized fitness= -5.4 
|- 
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OMP_AN:34997
|

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OMP:0006044
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decreased resistance to beta-lactam
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Relative to: OMP_AN:27535

<hr>Genotype differences:<br>
+lacZ4787(del)( +malF729(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Cefsulodin sodium salt hydrate
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CHEBI:31380) Cefsulodin sodium salt hydrate
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*chemical concentration=  12 ug/ml
*normalized fitness= -4.6 
|- 
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OMP_AN:34998
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OMP:0007886
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decreased resistance to a metal
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Relative to: OMP_AN:27579

<hr>Genotype differences:<br>
+lacZ4787(del)( +malF729(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Copper stress-CuCl2 dihydrate
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
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PMID:21185072
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towards some (CHEBI:86318) Copper stress-CuCl2 dihydrate
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*chemical concentration=  4 mM (LB)
*normalized fitness= -3.15 
|- 
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OMP_AN:34999
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OMP:0007878
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increased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27506

<hr>Genotype differences:<br>
+lacZ4787(del)( +malF729(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Doxorubicin hydrochloride
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
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PMID:21185072
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towards some (CHEBI:31522) Doxorubicin hydrochloride
|

*chemical concentration=  10 ug/ml
*normalized fitness= 3.73 
|- 
|
OMP_AN:35000
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OMP:0007075
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decreased growth factor requirement
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Relative to: OMP_AN:27605

<hr>Genotype differences:<br>
+lacZ4787(del)( +malF729(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-(+) Maltose monohydrate
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
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PMID:21185072
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towards some (CID:23615261) D-(+) Maltose monohydrate
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*chemical concentration= 0.1%
*normalized fitness= -13.62 
|- 
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OMP_AN:35001
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OMP:0007070
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decreased organic carbon source utilization
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Relative to: OMP_AN:27615

<hr>Genotype differences:<br>
+lacZ4787(del)( +malF729(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-(+) Maltose monohydrate
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CID:23615261) D-(+) Maltose monohydrate
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*chemical concentration= 0.1%
*normalized fitness= -13.62 
|- 
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OMP_AN:35002
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OMP:0007250
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increased resistance to a chemical
|
Relative to: OMP_AN:27570

<hr>Genotype differences:<br>
+lacZ4787(del)( +malF729(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
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*temperature:37C
*medium:LB agar
*medium:Propidium iodide
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
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PMID:21185072
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towards some (CHEBI:51240) Propidium iodide
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*chemical concentration=  50 ug/ml
*normalized fitness= 3.85 
|- 
|
OMP_AN:35003
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OMP:0007250
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increased resistance to a chemical
|
Relative to: OMP_AN:27573

<hr>Genotype differences:<br>
+lacZ4787(del)( +malF729(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Procaine hydrochloride
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CHEBI:8431) Procaine hydrochloride
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*chemical concentration=  30 mM
*normalized fitness= 3.57 

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==Additional Information==


==Links==


==References==
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