View source for OMP ST:73680 ! Escherichia coli K-12 JW3863

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==Strain Summary==
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!align=left align='left' bgcolor='#CCCCFF' |Strain Name
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Escherichia coli K-12 JW3863
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!align=left align='left' bgcolor='#CCCCFF' |Synonyms
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JW3863-1
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!align=left align='left' bgcolor='#CCCCFF' |Taxon Information
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*Pangenome: Escherichia coli
*Subspecies and/or strain: K-12
*NCBI Taxonomy ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
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!align=left align='left' bgcolor='#CCCCFF' |Genotype
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DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 fdoI755(del)::FRT-kan-FRT
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!align=left align='left' bgcolor='#CCCCFF' |Strain Reference
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*PMID:16738554
*[http://cgsc2.biology.yale.edu/Strain.php?ID=109070 Coli Genetic Stock Center]
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!align=left align='left' bgcolor='#CCCCFF' |Strain availability
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[http://cgsc2.biology.yale.edu/Strain.php?ID=109070 Coli Genetic Stock Center]
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!align=left align='left' bgcolor='#CCCCFF' |Ancestry
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parent: [[OMP ST:800 ! Escherichia coli K-12 BW25113]]
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!align=left align='left' bgcolor='#CCCCFF' |Annotated phenotypes
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==Notes==


==Phenotype Annotation==
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!|Annotation ID!!Qualifier!!OMP ID!!OMP term name!!Relative phenotype information!!Experimental condition!!ECO ID!!ECO term name!!Reference!!Annotation Extension!!Notes
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OMP_AN:34523
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|
OMP:0007250
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increased resistance to a chemical
|
Relative to: OMP_AN:27501

<hr>Genotype differences:<br>
+lacZ4787(del)( +fdoI755(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Dibucaine
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CHEBI:247956) Dibucaine
|

*chemical concentration=  1.2 mM
*normalized fitness= 4.46 
|- 
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OMP_AN:34524
|

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OMP:0007879
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decreased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27555

<hr>Genotype differences:<br>
+lacZ4787(del)( +fdoI755(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Fosfomycin disodium salt
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CID:6093162) Fosfomycin disodium salt
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*chemical concentration= 1 ug/ml
*normalized fitness= -3.55 
|- 
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OMP_AN:34525
|

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OMP:0007075
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decreased growth factor requirement
|
Relative to: OMP_AN:27603

<hr>Genotype differences:<br>
+lacZ4787(del)( +fdoI755(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-Glucose anhydrous
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:42758) D-Glucose anhydrous
|

*chemical concentration= 0.2%
*normalized fitness= -3.15 
|- 
|
OMP_AN:34526
|

|
OMP:0007070
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decreased organic carbon source utilization
|
Relative to: OMP_AN:27613

<hr>Genotype differences:<br>
+lacZ4787(del)( +fdoI755(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-Glucose anhydrous
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:42758) D-Glucose anhydrous
|

*chemical concentration= 0.2%
*normalized fitness= -3.15 
|- 
|
OMP_AN:34527
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27600

<hr>Genotype differences:<br>
+lacZ4787(del)( +fdoI755(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:NH4Cl
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CHEBI:31206) NH4Cl
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*chemical concentration= 9.5mm
*normalized fitness= -4.99 
|- 
|
OMP_AN:34528
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OMP:0007032
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decreased utilization of inorganic nitrogen source
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Relative to: OMP_AN:27610

<hr>Genotype differences:<br>
+lacZ4787(del)( +fdoI755(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:NH4Cl
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:31206) NH4Cl
|

*chemical concentration= 9.5mm
*normalized fitness= -4.99 
|- 
|
OMP_AN:34529
|

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OMP:0007886
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decreased resistance to a metal
|
Relative to: OMP_AN:27580

<hr>Genotype differences:<br>
+lacZ4787(del)( +fdoI755(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Nickel stress-Ni(II)Cl2 hexahydrate
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CHEBI:53542) Nickel stress-Ni(II)Cl2 hexahydrate
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*chemical concentration=  1 mM (LB)
*normalized fitness= -3.97 
|- 
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OMP_AN:34530
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OMP:0007896
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decreased resistance to a surfactant
|
Relative to: OMP_AN:27582

<hr>Genotype differences:<br>
+lacZ4787(del)( +fdoI755(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:SDS
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
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PMID:21185072
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towards some (CHEBI:8984) SDS
|

*chemical concentration= 0.5 %
*normalized fitness= -3.35 
|- 
|
OMP_AN:34531
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OMP:0007075
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decreased growth factor requirement
|
Relative to: OMP_AN:27598

<hr>Genotype differences:<br>
+lacZ4787(del)( +fdoI755(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron excess-FeSO4 heptahydride
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
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PMID:21185072
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towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride
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*chemical concentration= 1mM (MOPS; normal 100uM)
*normalized fitness= -4.97 
|- 
|
OMP_AN:34532
|

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OMP:0007886
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decreased resistance to a metal
|
Relative to: OMP_AN:27608

<hr>Genotype differences:<br>
+lacZ4787(del)( +fdoI755(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron excess-FeSO4 heptahydride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride
|

*chemical concentration= 1mM (MOPS; normal 100uM)
*normalized fitness= -4.97 
|- 
|
OMP_AN:34533
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27599

<hr>Genotype differences:<br>
+lacZ4787(del)( +fdoI755(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron starvation-FeSO4·7H2O
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O
|

*chemical concentration= 2uM (MOPS; normal 100uM)
*normalized fitness= -4.03 
|- 
|
OMP_AN:34534
|

|
OMP:0007937
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decreased resistance to nutrient limitation
|
Relative to: OMP_AN:27609

<hr>Genotype differences:<br>
+lacZ4787(del)( +fdoI755(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron starvation-FeSO4·7H2O
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O
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*chemical concentration= 2uM (MOPS; normal 100uM)
*normalized fitness= -4.03 

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==Additional Information==


==Links==


==References==
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