View source for OMP ST:73025 ! Escherichia coli K-12 JW2733

__TOC__

==Strain Summary==
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|-
!align=left align='left' bgcolor='#CCCCFF' |Strain Name
||
Escherichia coli K-12 JW2733
|-
!align=left align='left' bgcolor='#CCCCFF' |Synonyms
||
JW2733-1
|-
!align=left align='left' bgcolor='#CCCCFF' |Taxon Information
||
*Pangenome: Escherichia coli
*Subspecies and/or strain: K-12
*NCBI Taxonomy ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
|-
!align=left align='left' bgcolor='#CCCCFF' |Genotype
||
DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 cysI765(del)::FRT-kan-FRT
|-
!align=left align='left' bgcolor='#CCCCFF' |Strain Reference
||
*PMID:16738554
*[http://cgsc2.biology.yale.edu/Strain.php?ID=108430 Coli Genetic Stock Center]
|-
!align=left align='left' bgcolor='#CCCCFF' |Strain availability
||
[http://cgsc2.biology.yale.edu/Strain.php?ID=108430 Coli Genetic Stock Center]
|-
!align=left align='left' bgcolor='#CCCCFF' |Ancestry
||
parent: [[OMP ST:800 ! Escherichia coli K-12 BW25113]]
|-
!align=left align='left' bgcolor='#CCCCFF' |Annotated phenotypes
||


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==Notes==


==Phenotype Annotation==
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!|Annotation ID!!Qualifier!!OMP ID!!OMP term name!!Relative phenotype information!!Experimental condition!!ECO ID!!ECO term name!!Reference!!Annotation Extension!!Notes
|- 
|
OMP_AN:32436
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27601

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysI765(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Sodium Acetate anhydrous
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:32954) Sodium Acetate anhydrous
|

*chemical concentration= 0.6%
*normalized fitness= -15.72 
|- 
|
OMP_AN:32437
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27611

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysI765(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Sodium Acetate anhydrous
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:32954) Sodium Acetate anhydrous
|

*chemical concentration= 0.6%
*normalized fitness= -15.72 
|- 
|
OMP_AN:32438
|

|
OMP:0007896
|
decreased resistance to a surfactant
|
Relative to: OMP_AN:27581

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysI765(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Benzalkonium chloride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:3020) Benzalkonium chloride
|

*chemical concentration=  25 ug/ml
*normalized fitness= -3.4 
|- 
|
OMP_AN:32439
|

|
OMP:0007879
|
decreased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27556

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysI765(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Bicyclomycin
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:60584) Bicyclomycin
|

*chemical concentration=  10 ug/ml
*normalized fitness= -4.27 
|- 
|
OMP_AN:32440
|

|
OMP:0007879
|
decreased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27515

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysI765(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Fusidic acid sodium salt
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:126963539) Fusidic acid sodium salt
|

*chemical concentration=  50 ug/ml
*normalized fitness= -3.64 
|- 
|
OMP_AN:32441
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27606

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysI765(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:N-Acetyl-D-glucosamine
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:506227) N-Acetyl-D-glucosamine
|

*chemical concentration= 0.15%
*normalized fitness= -17.12 
|- 
|
OMP_AN:32442
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27616

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysI765(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:N-Acetyl-D-glucosamine
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:506227) N-Acetyl-D-glucosamine
|

*chemical concentration= 0.15%
*normalized fitness= -17.12 
|- 
|
OMP_AN:32443
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27602

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysI765(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-(+) Glucosamine hydrochloride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:91431) D-(+) Glucosamine hydrochloride
|

*chemical concentration= 0.2%
*normalized fitness= -26.31 
|- 
|
OMP_AN:32444
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27612

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysI765(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-(+) Glucosamine hydrochloride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:91431) D-(+) Glucosamine hydrochloride
|

*chemical concentration= 0.2%
*normalized fitness= -26.31 
|- 
|
OMP_AN:32445
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27603

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysI765(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-Glucose anhydrous
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:42758) D-Glucose anhydrous
|

*chemical concentration= 0.2%
*normalized fitness= -13.25 
|- 
|
OMP_AN:32446
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27613

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysI765(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-Glucose anhydrous
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:42758) D-Glucose anhydrous
|

*chemical concentration= 0.2%
*normalized fitness= -13.25 
|- 
|
OMP_AN:32447
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27604

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysI765(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Glycerol
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:17754) Glycerol
|

*chemical concentration= 0.4%
*normalized fitness= -19.36 
|- 
|
OMP_AN:32448
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27614

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysI765(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Glycerol
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:17754) Glycerol
|

*chemical concentration= 0.4%
*normalized fitness= -19.36 
|- 
|
OMP_AN:32449
|

|
OMP:0007377
|
decreased resistance to an alkylating agent
|
Relative to: OMP_AN:27502

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysI765(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:methyl methanesulfonate (MMS)
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:25255) methyl methanesulfonate (MMS)
|

*chemical concentration= 0.05%
*normalized fitness= -4.07 
|- 
|
OMP_AN:32450
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27605

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysI765(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-(+) Maltose monohydrate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:23615261) D-(+) Maltose monohydrate
|

*chemical concentration= 0.1%
*normalized fitness= -14.03 
|- 
|
OMP_AN:32451
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27615

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysI765(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-(+) Maltose monohydrate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:23615261) D-(+) Maltose monohydrate
|

*chemical concentration= 0.1%
*normalized fitness= -14.03 
|- 
|
OMP_AN:32452
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27600

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysI765(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:NH4Cl
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:31206) NH4Cl
|

*chemical concentration= 9.5mm
*normalized fitness= -24.84 
|- 
|
OMP_AN:32453
|

|
OMP:0007032
|
decreased utilization of inorganic nitrogen source
|
Relative to: OMP_AN:27610

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysI765(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:NH4Cl
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:31206) NH4Cl
|

*chemical concentration= 9.5mm
*normalized fitness= -24.84 
|- 
|
OMP_AN:32454
|

|
OMP:0007249
|
decreased resistance to a chemical
|
Relative to: OMP_AN:27513

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysI765(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Phenazine methosulfate (PMS)
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:8055) Phenazine methosulfate (PMS)
|

*chemical concentration= 0.02 mM
*normalized fitness= -3.51 
|- 
|
OMP_AN:32455
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27607

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysI765(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Succinic acid
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:15741) Succinic acid
|

*chemical concentration= 0.3%
*normalized fitness= -7.93 
|- 
|
OMP_AN:32456
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27617

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysI765(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Succinic acid
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:15741) Succinic acid
|

*chemical concentration= 0.3%
*normalized fitness= -7.93 
|- 
|
OMP_AN:32457
|

|
OMP:0007879
|
decreased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27588

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysI765(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Triclosan/Irgasan
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:164200) Triclosan/Irgasan
|

*chemical concentration= 0.05 ug/ml
*normalized fitness= -4.61 
|- 
|
OMP_AN:32458
|

|
OMP:0007879
|
decreased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27566

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysI765(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Vancomycin HCl hydrate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:9932) Vancomycin HCl hydrate
|

*chemical concentration= 10 ug/ml
*normalized fitness= -3.64 
|- 
|
OMP_AN:32459
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27598

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysI765(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron excess-FeSO4 heptahydride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride
|

*chemical concentration= 1mM (MOPS; normal 100uM)
*normalized fitness= -19.9 
|- 
|
OMP_AN:32460
|

|
OMP:0007886
|
decreased resistance to a metal
|
Relative to: OMP_AN:27608

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysI765(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron excess-FeSO4 heptahydride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride
|

*chemical concentration= 1mM (MOPS; normal 100uM)
*normalized fitness= -19.9 
|- 
|
OMP_AN:32461
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27599

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysI765(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron starvation-FeSO4·7H2O
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O
|

*chemical concentration= 2uM (MOPS; normal 100uM)
*normalized fitness= -18.28 
|- 
|
OMP_AN:32462
|

|
OMP:0007937
|
decreased resistance to nutrient limitation
|
Relative to: OMP_AN:27609

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysI765(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron starvation-FeSO4·7H2O
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O
|

*chemical concentration= 2uM (MOPS; normal 100uM)
*normalized fitness= -18.28 
|- 
|
OMP_AN:32463
|

|
OMP:0006040
|
decreased resistance to quinolone
|
Relative to: OMP_AN:27541

<hr>Genotype differences:<br>
+lacZ4787(del)( +cysI765(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Nalidixic acid sodium salt
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:3864541) Nalidixic acid sodium salt
|

*chemical concentration=  2 ug/ml
*normalized fitness= -3.19 

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==Additional Information==


==Links==


==References==
{{RefHelp}}<references/>