View source for OMP ST:72729 ! Escherichia coli K-12 JW2326

__TOC__

==Strain Summary==
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{|   id="C5b5106f135f49"  class=" tableEdit Strain_info_table"  

|-
!align=left align='left' bgcolor='#CCCCFF' |Strain Name
||
Escherichia coli K-12 JW2326
|-
!align=left align='left' bgcolor='#CCCCFF' |Synonyms
||
JW2326-1
|-
!align=left align='left' bgcolor='#CCCCFF' |Taxon Information
||
*Pangenome: Escherichia coli
*Subspecies and/or strain: K-12
*NCBI Taxonomy ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
|-
!align=left align='left' bgcolor='#CCCCFF' |Genotype
||
DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 aroC737(del)::FRT-kan-FRT
|-
!align=left align='left' bgcolor='#CCCCFF' |Strain Reference
||
*PMID:16738554
*[http://cgsc2.biology.yale.edu/Strain.php?ID=108146 Coli Genetic Stock Center]
|-
!align=left align='left' bgcolor='#CCCCFF' |Strain availability
||
[http://cgsc2.biology.yale.edu/Strain.php?ID=108146 Coli Genetic Stock Center]
|-
!align=left align='left' bgcolor='#CCCCFF' |Ancestry
||
parent: [[OMP ST:800 ! Escherichia coli K-12 BW25113]]
|-
!align=left align='left' bgcolor='#CCCCFF' |Annotated phenotypes
||


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==Notes==


==Phenotype Annotation==
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!|Annotation ID!!Qualifier!!OMP ID!!OMP term name!!Relative phenotype information!!Experimental condition!!ECO ID!!ECO term name!!Reference!!Annotation Extension!!Notes
|- 
|
OMP_AN:31524
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27601

<hr>Genotype differences:<br>
+lacZ4787(del)( +aroC737(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Sodium Acetate anhydrous
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:32954) Sodium Acetate anhydrous
|

*chemical concentration= 0.6%
*normalized fitness= -12.02 
|- 
|
OMP_AN:31525
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27611

<hr>Genotype differences:<br>
+lacZ4787(del)( +aroC737(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Sodium Acetate anhydrous
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:32954) Sodium Acetate anhydrous
|

*chemical concentration= 0.6%
*normalized fitness= -12.02 
|- 
|
OMP_AN:31526
|

|
OMP:0007249
|
decreased resistance to a chemical
|
Relative to: OMP_AN:27575

<hr>Genotype differences:<br>
+lacZ4787(del)( +aroC737(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Acriflavine (acriflavinium chloride)
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:74728) Acriflavine (acriflavinium chloride)
|

*chemical concentration= 2 ug/ml
*normalized fitness= -3.56 
|- 
|
OMP_AN:31527
|

|
OMP:0007249
|
decreased resistance to a chemical
|
Relative to: OMP_AN:27511

<hr>Genotype differences:<br>
+lacZ4787(del)( +aroC737(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:(-)-Epinephrine
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:28918) (-)-Epinephrine
|

*chemical concentration=  250 uM
*normalized fitness= -4.92 
|- 
|
OMP_AN:31528
|

|
OMP:0007879
|
decreased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27555

<hr>Genotype differences:<br>
+lacZ4787(del)( +aroC737(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Fosfomycin disodium salt
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:6093162) Fosfomycin disodium salt
|

*chemical concentration= 1 ug/ml
*normalized fitness= -6.01 
|- 
|
OMP_AN:31529
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27606

<hr>Genotype differences:<br>
+lacZ4787(del)( +aroC737(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:N-Acetyl-D-glucosamine
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:506227) N-Acetyl-D-glucosamine
|

*chemical concentration= 0.15%
*normalized fitness= -8.77 
|- 
|
OMP_AN:31530
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27616

<hr>Genotype differences:<br>
+lacZ4787(del)( +aroC737(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:N-Acetyl-D-glucosamine
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:506227) N-Acetyl-D-glucosamine
|

*chemical concentration= 0.15%
*normalized fitness= -8.77 
|- 
|
OMP_AN:31531
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27602

<hr>Genotype differences:<br>
+lacZ4787(del)( +aroC737(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-(+) Glucosamine hydrochloride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:91431) D-(+) Glucosamine hydrochloride
|

*chemical concentration= 0.2%
*normalized fitness= -20.29 
|- 
|
OMP_AN:31532
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27612

<hr>Genotype differences:<br>
+lacZ4787(del)( +aroC737(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-(+) Glucosamine hydrochloride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:91431) D-(+) Glucosamine hydrochloride
|

*chemical concentration= 0.2%
*normalized fitness= -20.29 
|- 
|
OMP_AN:31533
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27603

<hr>Genotype differences:<br>
+lacZ4787(del)( +aroC737(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-Glucose anhydrous
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:42758) D-Glucose anhydrous
|

*chemical concentration= 0.2%
*normalized fitness= -14.99 
|- 
|
OMP_AN:31534
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27613

<hr>Genotype differences:<br>
+lacZ4787(del)( +aroC737(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-Glucose anhydrous
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:42758) D-Glucose anhydrous
|

*chemical concentration= 0.2%
*normalized fitness= -14.99 
|- 
|
OMP_AN:31535
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27604

<hr>Genotype differences:<br>
+lacZ4787(del)( +aroC737(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Glycerol
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:17754) Glycerol
|

*chemical concentration= 0.4%
*normalized fitness= -19.24 
|- 
|
OMP_AN:31536
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27614

<hr>Genotype differences:<br>
+lacZ4787(del)( +aroC737(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Glycerol
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:17754) Glycerol
|

*chemical concentration= 0.4%
*normalized fitness= -19.24 
|- 
|
OMP_AN:31537
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27605

<hr>Genotype differences:<br>
+lacZ4787(del)( +aroC737(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-(+) Maltose monohydrate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:23615261) D-(+) Maltose monohydrate
|

*chemical concentration= 0.1%
*normalized fitness= -10.17 
|- 
|
OMP_AN:31538
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27615

<hr>Genotype differences:<br>
+lacZ4787(del)( +aroC737(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-(+) Maltose monohydrate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:23615261) D-(+) Maltose monohydrate
|

*chemical concentration= 0.1%
*normalized fitness= -10.17 
|- 
|
OMP_AN:31539
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27600

<hr>Genotype differences:<br>
+lacZ4787(del)( +aroC737(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:NH4Cl
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:31206) NH4Cl
|

*chemical concentration= 9.5mm
*normalized fitness= -17.77 
|- 
|
OMP_AN:31540
|

|
OMP:0007032
|
decreased utilization of inorganic nitrogen source
|
Relative to: OMP_AN:27610

<hr>Genotype differences:<br>
+lacZ4787(del)( +aroC737(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:NH4Cl
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:31206) NH4Cl
|

*chemical concentration= 9.5mm
*normalized fitness= -17.77 
|- 
|
OMP_AN:31541
|

|
OMP:0007249
|
decreased resistance to a chemical
|
Relative to: OMP_AN:27593

<hr>Genotype differences:<br>
+lacZ4787(del)( +aroC737(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:(-)-Norepinephrine
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:18357) (-)-Norepinephrine
|

*chemical concentration= 100 uM
*normalized fitness= -5.5 
|- 
|
OMP_AN:31542
|

|
OMP:0007879
|
decreased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27546

<hr>Genotype differences:<br>
+lacZ4787(del)( +aroC737(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Pyocyanin
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:8653) Pyocyanin
|

*chemical concentration= 0.2 ug/ml
*normalized fitness= -3.5 
|- 
|
OMP_AN:31543
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27607

<hr>Genotype differences:<br>
+lacZ4787(del)( +aroC737(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Succinic acid
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:15741) Succinic acid
|

*chemical concentration= 0.3%
*normalized fitness= -7.26 
|- 
|
OMP_AN:31544
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27617

<hr>Genotype differences:<br>
+lacZ4787(del)( +aroC737(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Succinic acid
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:15741) Succinic acid
|

*chemical concentration= 0.3%
*normalized fitness= -7.26 
|- 
|
OMP_AN:31545
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27598

<hr>Genotype differences:<br>
+lacZ4787(del)( +aroC737(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron excess-FeSO4 heptahydride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride
|

*chemical concentration= 1mM (MOPS; normal 100uM)
*normalized fitness= -18.83 
|- 
|
OMP_AN:31546
|

|
OMP:0007886
|
decreased resistance to a metal
|
Relative to: OMP_AN:27608

<hr>Genotype differences:<br>
+lacZ4787(del)( +aroC737(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron excess-FeSO4 heptahydride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride
|

*chemical concentration= 1mM (MOPS; normal 100uM)
*normalized fitness= -18.83 
|- 
|
OMP_AN:31547
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27599

<hr>Genotype differences:<br>
+lacZ4787(del)( +aroC737(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron starvation-FeSO4·7H2O
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O
|

*chemical concentration= 2uM (MOPS; normal 100uM)
*normalized fitness= -18.88 
|- 
|
OMP_AN:31548
|

|
OMP:0007937
|
decreased resistance to nutrient limitation
|
Relative to: OMP_AN:27609

<hr>Genotype differences:<br>
+lacZ4787(del)( +aroC737(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron starvation-FeSO4·7H2O
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O
|

*chemical concentration= 2uM (MOPS; normal 100uM)
*normalized fitness= -18.88 
|- 
|
OMP_AN:31549
|

|
OMP:0006037
|
increased resistance to macrolide
|
Relative to: OMP_AN:27521

<hr>Genotype differences:<br>
+lacZ4787(del)( +aroC737(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Spiramycin
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:6419898) Spiramycin
|

*chemical concentration=  20 ug/ml
*normalized fitness= 4.83 

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==Additional Information==


==Links==


==References==
{{RefHelp}}<references/>