View source for OMP ST:72698 ! Escherichia coli K-12 JW2277

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==Strain Summary==
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!align=left align='left' bgcolor='#CCCCFF' |Strain Name
||
Escherichia coli K-12 JW2277
|-
!align=left align='left' bgcolor='#CCCCFF' |Synonyms
||
JW2277-1
|-
!align=left align='left' bgcolor='#CCCCFF' |Taxon Information
||
*Pangenome: Escherichia coli
*Subspecies and/or strain: K-12
*NCBI Taxonomy ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
|-
!align=left align='left' bgcolor='#CCCCFF' |Genotype
||
DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 nuoH764(del)::FRT-kan-FRT
|-
!align=left align='left' bgcolor='#CCCCFF' |Strain Reference
||
*PMID:16738554
*[http://cgsc2.biology.yale.edu/Strain.php?ID=108116 Coli Genetic Stock Center]
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!align=left align='left' bgcolor='#CCCCFF' |Strain availability
||
[http://cgsc2.biology.yale.edu/Strain.php?ID=108116 Coli Genetic Stock Center]
|-
!align=left align='left' bgcolor='#CCCCFF' |Ancestry
||
parent: [[OMP ST:800 ! Escherichia coli K-12 BW25113]]
|-
!align=left align='left' bgcolor='#CCCCFF' |Annotated phenotypes
||


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==Notes==


==Phenotype Annotation==
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!|Annotation ID!!Qualifier!!OMP ID!!OMP term name!!Relative phenotype information!!Experimental condition!!ECO ID!!ECO term name!!Reference!!Annotation Extension!!Notes
|- 
|
OMP_AN:31360
|

|
OMP:0007249
|
decreased resistance to a chemical
|
Relative to: OMP_AN:27594

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoH764(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:SDS (CHEBI:8984) +EDTA (CHEBI:64758)
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:64758 + CHEBI:8984) SDS (CHEBI:8984) +EDTA (CHEBI:64758)
|

*chemical concentration=  0.5%/0.5mM %/0.5mM
*normalized fitness= -10.43 
|- 
|
OMP_AN:31361
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27601

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoH764(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Sodium Acetate anhydrous
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:32954) Sodium Acetate anhydrous
|

*chemical concentration= 0.6%
*normalized fitness= -3.1 
|- 
|
OMP_AN:31362
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27611

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoH764(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Sodium Acetate anhydrous
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:32954) Sodium Acetate anhydrous
|

*chemical concentration= 0.6%
*normalized fitness= -3.1 
|- 
|
OMP_AN:31363
|

|
OMP:0007249
|
decreased resistance to a chemical
|
Relative to: OMP_AN:27575

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoH764(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Acriflavine (acriflavinium chloride)
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:74728) Acriflavine (acriflavinium chloride)
|

*chemical concentration= 2 ug/ml
*normalized fitness= -11.77 
|- 
|
OMP_AN:31364
|

|
OMP:0007896
|
decreased resistance to a surfactant
|
Relative to: OMP_AN:27581

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoH764(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Benzalkonium chloride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:3020) Benzalkonium chloride
|

*chemical concentration=  10 ug/ml
*normalized fitness= -3.74 
|- 
|
OMP_AN:31365
|

|
OMP:0007249
|
decreased resistance to a chemical
|
Relative to: OMP_AN:27571

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoH764(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:CCCP (Carbonyl cyanide 3-chlorophenylhydrazone)
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:3259) CCCP (Carbonyl cyanide 3-chlorophenylhydrazone)
|

*chemical concentration=  2 ug/ml
*normalized fitness= -4.1 
|- 
|
OMP_AN:31366
|

|
OMP:0007903
|
decreased resistance to bile acids
|
Relative to: OMP_AN:27585

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoH764(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Cholate sodium hydrate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:23679061) Cholate sodium hydrate
|

*chemical concentration=  0.5 %
*normalized fitness= -3.27 
|- 
|
OMP_AN:31367
|

|
OMP:0007886
|
decreased resistance to a metal
|
Relative to: OMP_AN:27579

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoH764(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Copper stress-CuCl2 dihydrate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:86318) Copper stress-CuCl2 dihydrate
|

*chemical concentration= 1 mM (LB)
*normalized fitness= -6.47 
|- 
|
OMP_AN:31368
|

|
OMP:0007912
|
decreased resistance to a chelator
|
Relative to: OMP_AN:27507

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoH764(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:EDTA disodium salt dihydrate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:64758) EDTA disodium salt dihydrate
|

*chemical concentration=   1 mM
*normalized fitness= -5.13 
|- 
|
OMP_AN:31369
|

|
OMP:0007879
|
decreased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27555

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoH764(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Fosfomycin disodium salt
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:6093162) Fosfomycin disodium salt
|

*chemical concentration= 1 ug/ml
*normalized fitness= -4.72 
|- 
|
OMP_AN:31370
|

|
OMP:0007879
|
decreased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27515

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoH764(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Fusidic acid sodium salt
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:126963539) Fusidic acid sodium salt
|

*chemical concentration=  50 ug/ml
*normalized fitness= -3.46 
|- 
|
OMP_AN:31371
|

|
OMP:0007074
|
increased growth factor requirement
|
Relative to: OMP_AN:27602

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoH764(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-(+) Glucosamine hydrochloride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:91431) D-(+) Glucosamine hydrochloride
|

*chemical concentration= 0.2%
*normalized fitness= 3.06 
|- 
|
OMP_AN:31372
|

|
OMP:0007071
|
increased organic carbon source utilization
|
Relative to: OMP_AN:27612

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoH764(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-(+) Glucosamine hydrochloride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:91431) D-(+) Glucosamine hydrochloride
|

*chemical concentration= 0.2%
*normalized fitness= 3.06 
|- 
|
OMP_AN:31373
|

|
OMP:0007886
|
decreased resistance to a metal
|
Relative to: OMP_AN:27580

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoH764(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Nickel stress-Ni(II)Cl2 hexahydrate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:53542) Nickel stress-Ni(II)Cl2 hexahydrate
|

*chemical concentration=  1 mM (LB)
*normalized fitness= -4.81 
|- 
|
OMP_AN:31374
|

|
OMP:0007249
|
decreased resistance to a chemical
|
Relative to: OMP_AN:27570

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoH764(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Propidium iodide
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:51240) Propidium iodide
|

*chemical concentration=  20 ug/ml
*normalized fitness= -3.97 
|- 
|
OMP_AN:31375
|

|
OMP:0007879
|
decreased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27546

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoH764(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Pyocyanin
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:8653) Pyocyanin
|

*chemical concentration=  10 ug/ml
*normalized fitness= -6.05 
|- 
|
OMP_AN:31376
|

|
OMP:0007896
|
decreased resistance to a surfactant
|
Relative to: OMP_AN:27582

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoH764(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:SDS
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:8984) SDS
|

*chemical concentration= 0.5 %
*normalized fitness= -5.72 
|- 
|
OMP_AN:31377
|

|
OMP:0007903
|
decreased resistance to bile acids
|
Relative to: OMP_AN:27584

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoH764(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Taurocholic acid sodium salt hydrate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:23687511) Taurocholic acid sodium salt hydrate
|

*chemical concentration=  0.5 %
*normalized fitness= -3.84 
|- 
|
OMP_AN:31378
|

|
OMP:0007249
|
decreased resistance to a chemical
|
Relative to: OMP_AN:27567

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoH764(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Theophylline anhydrous
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:28177) Theophylline anhydrous
|

*chemical concentration=  100 ug/ml
*normalized fitness= -3.77 
|- 
|
OMP_AN:31379
|

|
OMP:0007249
|
decreased resistance to a chemical
|
Relative to: OMP_AN:27569

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoH764(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:EtOH
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:16236) EtOH
|

*chemical concentration=  4 %
*normalized fitness= -3.55 
|- 
|
OMP_AN:31380
|

|
OMP:0006040
|
decreased resistance to quinolone
|
Relative to: OMP_AN:27541

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoH764(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Nalidixic acid sodium salt
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:3864541) Nalidixic acid sodium salt
|

*chemical concentration=  2 ug/ml
*normalized fitness= -5.36 

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==Additional Information==


==Links==


==References==
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