View source for OMP ST:71779 ! Escherichia coli K-12 JW0863

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==Strain Summary==
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!align=left align='left' bgcolor='#CCCCFF' |Strain Name
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Escherichia coli K-12 JW0863
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!align=left align='left' bgcolor='#CCCCFF' |Synonyms
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JW0863-1
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!align=left align='left' bgcolor='#CCCCFF' |Taxon Information
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*Pangenome: Escherichia coli
*Subspecies and/or strain: K-12
*NCBI Taxonomy ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
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!align=left align='left' bgcolor='#CCCCFF' |Genotype
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DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 macB780(del)::FRT-kan-FRT
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!align=left align='left' bgcolor='#CCCCFF' |Strain Reference
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*PMID:16738554
*[http://cgsc2.biology.yale.edu/Strain.php?ID=107176 Coli Genetic Stock Center]
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!align=left align='left' bgcolor='#CCCCFF' |Strain availability
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[http://cgsc2.biology.yale.edu/Strain.php?ID=107176 Coli Genetic Stock Center]
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!align=left align='left' bgcolor='#CCCCFF' |Ancestry
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parent: [[OMP ST:800 ! Escherichia coli K-12 BW25113]]
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!align=left align='left' bgcolor='#CCCCFF' |Annotated phenotypes
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==Notes==


==Phenotype Annotation==
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!|Annotation ID!!Qualifier!!OMP ID!!OMP term name!!Relative phenotype information!!Experimental condition!!ECO ID!!ECO term name!!Reference!!Annotation Extension!!Notes
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OMP_AN:29356
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OMP:0007895
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increased resistance to a surfactant
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Relative to: OMP_AN:27581

<hr>Genotype differences:<br>
+lacZ4787(del)( +macB780(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Benzalkonium chloride
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
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PMID:21185072
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towards some (CHEBI:3020) Benzalkonium chloride
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*chemical concentration=  25 ug/ml
*normalized fitness= 3.33 
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OMP_AN:29357
|

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OMP:0007074
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increased growth factor requirement
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Relative to: OMP_AN:27600

<hr>Genotype differences:<br>
+lacZ4787(del)( +macB780(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
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*temperature:37C
*medium:MOPS
*medium:NH4Cl
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
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PMID:21185072
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towards some (CHEBI:31206) NH4Cl
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*chemical concentration= 9.5mm
*normalized fitness= 3.02 
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OMP_AN:29358
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OMP:0007033
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increased utilization of inorganic nitrogen source
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Relative to: OMP_AN:27610

<hr>Genotype differences:<br>
+lacZ4787(del)( +macB780(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:NH4Cl
|
ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CHEBI:31206) NH4Cl
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*chemical concentration= 9.5mm
*normalized fitness= 3.02 
|- 
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OMP_AN:29359
|

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OMP:0007888
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increased resistance to a metal
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Relative to: OMP_AN:27580

<hr>Genotype differences:<br>
+lacZ4787(del)( +macB780(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Nickel stress-Ni(II)Cl2 hexahydrate
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CHEBI:53542) Nickel stress-Ni(II)Cl2 hexahydrate
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*chemical concentration=  1 mM (LB)
*normalized fitness= 5.05 
|- 
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OMP_AN:29360
|

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OMP:0007250
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increased resistance to a chemical
|
Relative to: OMP_AN:27573

<hr>Genotype differences:<br>
+lacZ4787(del)( +macB780(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Procaine hydrochloride
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CHEBI:8431) Procaine hydrochloride
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*chemical concentration=  10 mM
*normalized fitness= 3.54 
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OMP_AN:29361
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OMP:0007912
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decreased resistance to a chelator
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Relative to: OMP_AN:27508

<hr>Genotype differences:<br>
+lacZ4787(del)( +macB780(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:EGTA
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
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PMID:21185072
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towards some (CHEBI:30740) EGTA
|

*chemical concentration=   2 mM
*normalized fitness= -3.49 
|- 
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OMP_AN:29362
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OMP:0007878
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increased resistance to an antimicrobial agent
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Relative to: OMP_AN:27591

<hr>Genotype differences:<br>
+lacZ4787(del)( +macB780(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Trimethoprim (CHEBI:45924)  + Sulfamethizole (CHEBI:9331)
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
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PMID:21185072
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towards some (CHEBI:45924 + CHEBI:9331) Trimethoprim (CHEBI:45924)  + Sulfamethizole (CHEBI:9331)
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*chemical concentration= 0.1 + 50 ug/ml
*normalized fitness= 5.15 

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==Additional Information==


==Links==


==References==
{{RefHelp}}<references/>