View source for OMP ST:71463 ! Escherichia coli K-12 JW0395

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==Strain Summary==
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!align=left align='left' bgcolor='#CCCCFF' |Strain Name
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Escherichia coli K-12 JW0395
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!align=left align='left' bgcolor='#CCCCFF' |Synonyms
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JW0395-2
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!align=left align='left' bgcolor='#CCCCFF' |Taxon Information
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*Pangenome: Escherichia coli
*Subspecies and/or strain: K-12
*NCBI Taxonomy ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
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!align=left align='left' bgcolor='#CCCCFF' |Genotype
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DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 queA769(del)::FRT-kan-FRT
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!align=left align='left' bgcolor='#CCCCFF' |Strain Reference
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*PMID:16738554
*[http://cgsc2.biology.yale.edu/Strain.php?ID=106851 Coli Genetic Stock Center]
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!align=left align='left' bgcolor='#CCCCFF' |Strain availability
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[http://cgsc2.biology.yale.edu/Strain.php?ID=106851 Coli Genetic Stock Center]
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!align=left align='left' bgcolor='#CCCCFF' |Ancestry
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parent: [[OMP ST:800 ! Escherichia coli K-12 BW25113]]
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!align=left align='left' bgcolor='#CCCCFF' |Annotated phenotypes
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==Notes==


==Phenotype Annotation==
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!|Annotation ID!!Qualifier!!OMP ID!!OMP term name!!Relative phenotype information!!Experimental condition!!ECO ID!!ECO term name!!Reference!!Annotation Extension!!Notes
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OMP_AN:28369
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OMP:0006036
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decreased resistance to macrolide
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Relative to: OMP_AN:27518

<hr>Genotype differences:<br>
+lacZ4787(del)( +queA769(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Azithromycin
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
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PMID:21185072
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towards some (CHEBI:2955) Azithromycin
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*chemical concentration=  1 ug/ml
*normalized fitness= -4.22 
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OMP_AN:28370
|

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OMP:0007903
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decreased resistance to bile acids
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Relative to: OMP_AN:27586

<hr>Genotype differences:<br>
+lacZ4787(del)( +queA769(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
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*temperature:37C
*medium:LB agar
*medium:Deoxycholate sodium
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
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PMID:21185072
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towards some (CHEBI:9177) Deoxycholate sodium
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*chemical concentration= 0.1 %
*normalized fitness= -3.59 
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OMP_AN:28371
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OMP:0007879
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decreased resistance to an antimicrobial agent
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Relative to: OMP_AN:27555

<hr>Genotype differences:<br>
+lacZ4787(del)( +queA769(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Fosfomycin disodium salt
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
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PMID:21185072
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towards some (CID:6093162) Fosfomycin disodium salt
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*chemical concentration= 1 ug/ml
*normalized fitness= -3.49 
|- 
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OMP_AN:28372
|

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OMP:0007377
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decreased resistance to an alkylating agent
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Relative to: OMP_AN:27502

<hr>Genotype differences:<br>
+lacZ4787(del)( +queA769(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:methyl methanesulfonate (MMS)
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CHEBI:25255) methyl methanesulfonate (MMS)
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*chemical concentration= 0.05%
*normalized fitness= -3.41 
|- 
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OMP_AN:28373
|

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OMP:0006045
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increased resistance to beta-lactam compound
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Relative to: OMP_AN:27537

<hr>Genotype differences:<br>
+lacZ4787(del)( +queA769(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Mecillinam
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
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PMID:21185072
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towards some (CHEBI:51208) Mecillinam
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*chemical concentration=  0.09 ug/ml
*normalized fitness= 3.39 
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OMP_AN:28374
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OMP:0007879
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decreased resistance to an antimicrobial agent
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Relative to: OMP_AN:27546

<hr>Genotype differences:<br>
+lacZ4787(del)( +queA769(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Pyocyanin
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CHEBI:8653) Pyocyanin
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*chemical concentration=  10 ug/ml
*normalized fitness= -6.07 
|- 
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OMP_AN:28375
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OMP:0007833
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decreased resistance to sulfonamde
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Relative to: OMP_AN:27577

<hr>Genotype differences:<br>
+lacZ4787(del)( +queA769(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Sulfamonomethoxine
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
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PMID:21185072
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towards some (CHEBI:32164) Sulfamonomethoxine
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*chemical concentration=  100 ug/ml
*normalized fitness= -3.86 
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OMP_AN:28376
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OMP:0007249
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decreased resistance to a chemical
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Relative to: OMP_AN:27569

<hr>Genotype differences:<br>
+lacZ4787(del)( +queA769(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
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*temperature:37C
*medium:LB agar
*medium:EtOH
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CHEBI:16236) EtOH
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*chemical concentration=  4 %
*normalized fitness= -4.37 

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==Additional Information==


==Links==


==References==
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