View source for OMP ST:71189 ! Escherichia coli K-12 GSO214

__TOC__

==Strain Summary==
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!align=left align='left' bgcolor='#CCCCFF' |Strain Name
||
Escherichia coli K-12 GSO214
|-
!align=left align='left' bgcolor='#CCCCFF' |Synonyms
||

|-
!align=left align='left' bgcolor='#CCCCFF' |Taxon Information
||
*Pangenome: Escherichia coli
*Subspecies and/or strain: K-12
*NCBI Taxonomy ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
|-
!align=left align='left' bgcolor='#CCCCFF' |Genotype
||
F- Lambda- rph-1 ybhU(del)::FRT-kan-FRT
|-
!align=left align='left' bgcolor='#CCCCFF' |Strain Reference
||
PMID:19734312
|-
!align=left align='left' bgcolor='#CCCCFF' |Strain availability
||

|-
!align=left align='left' bgcolor='#CCCCFF' |Ancestry
||
parent: [[OMP ST:159 ! Escherichia coli K-12 MG1655]]
|-
!align=left align='left' bgcolor='#CCCCFF' |Annotated phenotypes
||


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==Notes==


==Phenotype Annotation==
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="E5b5104306e4aa"  class=" tableEdit OMP_annotation_table dataTable"  
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!|Annotation ID!!Qualifier!!OMP ID!!OMP term name!!Relative phenotype information!!Experimental condition!!ECO ID!!ECO term name!!Reference!!Annotation Extension!!Notes
|- 
|
OMP_AN:36959
|

|
OMP:0007902
|
increased resistance to bile acids
|
Relative to: OMP_AN:27587

<hr>Genotype differences:<br>
+ybhU(del)  -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:26711 + CHEBI:9177) Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)
|

*chemical concentration=  2 %
*normalized fitness= 3.19 
|- 
|
OMP_AN:36960
|

|
OMP:0007879
|
decreased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27561

<hr>Genotype differences:<br>
+ybhU(del)  -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Bleomycin sulfate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:34582) Bleomycin sulfate
|

*chemical concentration=  0.5 ug/ml
*normalized fitness= -4.56 
|- 
|
OMP_AN:36961
|

|
OMP:0006045
|
increased resistance to beta-lactam compound
|
Relative to: OMP_AN:27534

<hr>Genotype differences:<br>
+ybhU(del)  -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Cefoxitin sodium hydrate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:3501) Cefoxitin sodium hydrate
|

*chemical concentration=  0.5 ug/ml
*normalized fitness= 3.47 
|- 
|
OMP_AN:36962
|

|
OMP:0007878
|
increased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27548

<hr>Genotype differences:<br>
+ybhU(del)  -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Cerulenin
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:171741) Cerulenin
|

*chemical concentration=  4 ug/ml
*normalized fitness= 4.35 
|- 
|
OMP_AN:36963
|

|
OMP:0007250
|
increased resistance to a chemical
|
Relative to: OMP_AN:27509

<hr>Genotype differences:<br>
+ybhU(del)  -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Cisplatin-cis-Diamineplatinum(II) dichloride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:27899) Cisplatin-cis-Diamineplatinum(II) dichloride
|

*chemical concentration=  100 ug/ml
*normalized fitness= 5.22 
|- 
|
OMP_AN:36964
|

|
OMP:0006037
|
increased resistance to macrolide
|
Relative to: OMP_AN:27519

<hr>Genotype differences:<br>
+ybhU(del)  -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Clarithromycin
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:3732) Clarithromycin
|

*chemical concentration=  1 ug/ml
*normalized fitness= 3.68 
|- 
|
OMP_AN:36965
|

|
OMP:0007250
|
increased resistance to a chemical
|
Relative to: OMP_AN:27501

<hr>Genotype differences:<br>
+ybhU(del)  -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Dibucaine
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:247956) Dibucaine
|

*chemical concentration=  0.8 mM
*normalized fitness= 4.6 
|- 
|
OMP_AN:36966
|

|
OMP:0007250
|
increased resistance to a chemical
|
Relative to: OMP_AN:27572

<hr>Genotype differences:<br>
+ybhU(del)  -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Epigallocatechin gallate (EGCG)
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:4806) Epigallocatechin gallate (EGCG)
|

*chemical concentration= 5 uM
*normalized fitness= 4.57 
|- 
|
OMP_AN:36967
|

|
OMP:0007878
|
increased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27515

<hr>Genotype differences:<br>
+ybhU(del)  -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Fusidic acid sodium salt
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:126963539) Fusidic acid sodium salt
|

*chemical concentration=  5 ug/ml
*normalized fitness= 3.76 
|- 
|
OMP_AN:36968
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27603

<hr>Genotype differences:<br>
+ybhU(del)  -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-Glucose anhydrous
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:42758) D-Glucose anhydrous
|

*chemical concentration= 0.2%
*normalized fitness= -2.99 
|- 
|
OMP_AN:36969
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27613

<hr>Genotype differences:<br>
+ybhU(del)  -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-Glucose anhydrous
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:42758) D-Glucose anhydrous
|

*chemical concentration= 0.2%
*normalized fitness= -2.99 
|- 
|
OMP_AN:36970
|

|
OMP:0007250
|
increased resistance to a chemical
|
Relative to: OMP_AN:27512

<hr>Genotype differences:<br>
+ybhU(del)  -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Hydroxyurea
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:44423) Hydroxyurea
|

*chemical concentration=  10 mM
*normalized fitness= 4.45 
|- 
|
OMP_AN:36971
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27605

<hr>Genotype differences:<br>
+ybhU(del)  -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-(+) Maltose monohydrate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:23615261) D-(+) Maltose monohydrate
|

*chemical concentration= 0.1%
*normalized fitness= -3.48 
|- 
|
OMP_AN:36972
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27615

<hr>Genotype differences:<br>
+ybhU(del)  -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-(+) Maltose monohydrate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:23615261) D-(+) Maltose monohydrate
|

*chemical concentration= 0.1%
*normalized fitness= -3.48 
|- 
|
OMP_AN:36973
|

|
OMP:0007249
|
decreased resistance to a chemical
|
Relative to: OMP_AN:27510

<hr>Genotype differences:<br>
+ybhU(del)  -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Mitomycin C
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:27504) Mitomycin C
|

*chemical concentration= 0.1 ug/ml
*normalized fitness= -5.53 
|- 
|
OMP_AN:36974
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27600

<hr>Genotype differences:<br>
+ybhU(del)  -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:NH4Cl
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:31206) NH4Cl
|

*chemical concentration= 9.5mm
*normalized fitness= -3.48 
|- 
|
OMP_AN:36975
|

|
OMP:0007032
|
decreased utilization of inorganic nitrogen source
|
Relative to: OMP_AN:27610

<hr>Genotype differences:<br>
+ybhU(del)  -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:NH4Cl
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:31206) NH4Cl
|

*chemical concentration= 9.5mm
*normalized fitness= -3.48 
|- 
|
OMP_AN:36976
|

|
OMP:0007250
|
increased resistance to a chemical
|
Relative to: OMP_AN:27574

<hr>Genotype differences:<br>
+ybhU(del)  -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Paraquat dichloride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:28786) Paraquat dichloride
|

*chemical concentration=  10 uM
*normalized fitness= 3.52 
|- 
|
OMP_AN:36977
|

|
OMP:0007878
|
increased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27588

<hr>Genotype differences:<br>
+ybhU(del)  -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Triclosan/Irgasan
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:164200) Triclosan/Irgasan
|

*chemical concentration= 0.05 ug/ml
*normalized fitness= 6.64 
|- 
|
OMP_AN:36978
|

|
OMP:0007895
|
increased resistance to a surfactant
|
Relative to: OMP_AN:27583

<hr>Genotype differences:<br>
+ybhU(del)  -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Triton X-100
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:9750) Triton X-100
|

*chemical concentration=  0.2 %
*normalized fitness= 3.82 
|- 
|
OMP_AN:36979
|

|
OMP:0007878
|
increased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27566

<hr>Genotype differences:<br>
+ybhU(del)  -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Vancomycin HCl hydrate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:9932) Vancomycin HCl hydrate
|

*chemical concentration=  20 ug/ml
*normalized fitness= 4.65 
|- 
|
OMP_AN:36980
|

|
OMP:0007250
|
increased resistance to a chemical
|
Relative to: OMP_AN:27568

<hr>Genotype differences:<br>
+ybhU(del)  -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:(±)-Verapamil hydrochloride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:53188) (±)-Verapamil hydrochloride
|

*chemical concentration=  0.5 mM
*normalized fitness= 5.86 
|- 
|
OMP_AN:36981
|

|
OMP:0007878
|
increased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27560

<hr>Genotype differences:<br>
+ybhU(del)  -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Bacitracin Zinc Sulfate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:16219032) Bacitracin Zinc Sulfate
|

*chemical concentration=  200 ug/ml
*normalized fitness= 3.56 
|- 
|
OMP_AN:36982
|

|
OMP:0007879
|
decreased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27544

<hr>Genotype differences:<br>
+ybhU(del)  -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Chloramphenicol
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:17698) Chloramphenicol
|

*chemical concentration=  1.5 ug/ml
*normalized fitness= -3.63 
|- 
|
OMP_AN:36983
|

|
OMP:0006063
|
increased resistance to tetracycline
|
Relative to: OMP_AN:27522

<hr>Genotype differences:<br>
+ybhU(del)  -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Doxycycline hyclate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:34730) Doxycycline hyclate
|

*chemical concentration=  1 ug/ml
*normalized fitness= 3.16 
|- 
|
OMP_AN:36984
|

|
OMP:0007249
|
decreased resistance to a chemical
|
Relative to: OMP_AN:27569

<hr>Genotype differences:<br>
+ybhU(del)  -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:EtOH
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:16236) EtOH
|

*chemical concentration=  4 %
*normalized fitness= -3.25 
|- 
|
OMP_AN:36985
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27598

<hr>Genotype differences:<br>
+ybhU(del)  -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron excess-FeSO4 heptahydride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride
|

*chemical concentration= 1mM (MOPS; normal 100uM)
*normalized fitness= -3.04 
|- 
|
OMP_AN:36986
|

|
OMP:0007886
|
decreased resistance to a metal
|
Relative to: OMP_AN:27608

<hr>Genotype differences:<br>
+ybhU(del)  -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron excess-FeSO4 heptahydride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride
|

*chemical concentration= 1mM (MOPS; normal 100uM)
*normalized fitness= -3.04 
|- 
|
OMP_AN:36987
|

|
OMP:0007878
|
increased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27565

<hr>Genotype differences:<br>
+ybhU(del)  -DE(araD-araB)567 -DE(rhaD-rhaB)568 -hsdR514 -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Polymyxin B sulfate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:8310) Polymyxin B sulfate
|

*chemical concentration= 1 ug/ml
*normalized fitness= 4.16 

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==Additional Information==


==Links==


==References==
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