View source for OMP ST:1071 ! Escherichia coli K-12 JW2279

__TOC__

==Strain Summary==
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!align=left align='left' bgcolor='#CCCCFF' |Strain Name
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Escherichia coli K-12 JW2279
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!align=left align='left' bgcolor='#CCCCFF' |Synonyms
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JW2279-3
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!align=left align='left' bgcolor='#CCCCFF' |Taxon Information
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*Pangenome: Escherichia coli 
*Subspecies and/or strain: K-12
*NCBI Taxonomy ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
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!align=left align='left' bgcolor='#CCCCFF' |Genotype
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DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 nuoF766(del)::kan
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!align=left align='left' bgcolor='#CCCCFF' |Strain Reference
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[http://cgsc.biology.yale.edu/Strain.php?ID=108118 Coli Genetic Stock Center]
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!align=left align='left' bgcolor='#CCCCFF' |Strain availability
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[http://cgsc.biology.yale.edu/Strain.php?ID=108118 Coli Genetic Stock Center]
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!align=left align='left' bgcolor='#CCCCFF' |Ancestry
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parent:OMP ST:800 ! Escherichia coli K-12 BW25113
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!align=left align='left' bgcolor='#CCCCFF' |Annotated phenotypes
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==Notes==


==Phenotype Annotation==
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!|Annotation ID!!Qualifier!!OMP ID!!OMP term name!!Relative phenotype information!!Experimental condition!!ECO ID!!ECO term name!!Reference!!Annotation Extension!!Notes
|- 
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OMP_AN:983
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OMP:0007092
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decreased cell motility by swarming
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Relative to: OMP_AN:881

<hr>Genotype differences:<br>
+DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +nuoF766(del)  -IN(rrnD-rrnE)1
<hr>Condition differences:<br>
no differences
|
*temperature:33C
*medium:swarming motility medium
|
ECO:0005803
|
motility assay evidence
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PMID:17122336
|

|
 
|- 
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OMP_AN:31383
|

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OMP:0007249
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decreased resistance to a chemical
|
Relative to: OMP_AN:27594

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoF766(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:SDS (CHEBI:8984) +EDTA (CHEBI:64758)
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ECO:0007005
|
high throughput direct assay evidence used in manual assertion
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PMID:21185072
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towards some (CHEBI:64758 + CHEBI:8984) SDS (CHEBI:8984) +EDTA (CHEBI:64758)
|

*chemical concentration=  0.5%/0.5mM %/0.5mM
*normalized fitness= -6.9 
|- 
|
OMP_AN:31384
|

|
OMP:0007249
|
decreased resistance to a chemical
|
Relative to: OMP_AN:27575

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoF766(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Acriflavine (acriflavinium chloride)
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:74728) Acriflavine (acriflavinium chloride)
|

*chemical concentration= 2 ug/ml
*normalized fitness= -6.96 
|- 
|
OMP_AN:31385
|

|
OMP:0006057
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decreased resistance to aminoglycoside
|
Relative to: OMP_AN:27525

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoF766(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Amikacin sulfate salt (disulfate)
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ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:2638) Amikacin sulfate salt (disulfate)
|

*chemical concentration=  0.1 ug/ml
*normalized fitness= -3.86 
|- 
|
OMP_AN:31386
|

|
OMP:0007249
|
decreased resistance to a chemical
|
Relative to: OMP_AN:27571

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoF766(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:CCCP (Carbonyl cyanide 3-chlorophenylhydrazone)
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CHEBI:3259) CCCP (Carbonyl cyanide 3-chlorophenylhydrazone)
|

*chemical concentration=  2 ug/ml
*normalized fitness= -3.78 
|- 
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OMP_AN:31387
|

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OMP:0007903
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decreased resistance to bile acids
|
Relative to: OMP_AN:27585

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoF766(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Cholate sodium hydrate
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CID:23679061) Cholate sodium hydrate
|

*chemical concentration=  1 %
*normalized fitness= -3.47 
|- 
|
OMP_AN:31388
|

|
OMP:0007886
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decreased resistance to a metal
|
Relative to: OMP_AN:27579

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoF766(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Copper stress-CuCl2 dihydrate
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CHEBI:86318) Copper stress-CuCl2 dihydrate
|

*chemical concentration= 1 mM (LB)
*normalized fitness= -5.56 
|- 
|
OMP_AN:31389
|

|
OMP:0007249
|
decreased resistance to a chemical
|
Relative to: OMP_AN:27501

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoF766(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Dibucaine
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CHEBI:247956) Dibucaine
|

*chemical concentration=  1.2 mM
*normalized fitness= -3.9 
|- 
|
OMP_AN:31390
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|
OMP:0007879
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decreased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27555

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoF766(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Fosfomycin disodium salt
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CID:6093162) Fosfomycin disodium salt
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*chemical concentration= 1 ug/ml
*normalized fitness= -5.12 
|- 
|
OMP_AN:31391
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OMP:0007074
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increased growth factor requirement
|
Relative to: OMP_AN:27602

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoF766(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-(+) Glucosamine hydrochloride
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CID:91431) D-(+) Glucosamine hydrochloride
|

*chemical concentration= 0.2%
*normalized fitness= 3.21 
|- 
|
OMP_AN:31392
|

|
OMP:0007071
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increased organic carbon source utilization
|
Relative to: OMP_AN:27612

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoF766(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-(+) Glucosamine hydrochloride
|
ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:91431) D-(+) Glucosamine hydrochloride
|

*chemical concentration= 0.2%
*normalized fitness= 3.21 
|- 
|
OMP_AN:31393
|

|
OMP:0007074
|
increased growth factor requirement
|
Relative to: OMP_AN:27600

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoF766(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:NH4Cl
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CHEBI:31206) NH4Cl
|

*chemical concentration= 9.5mm
*normalized fitness= 3.16 
|- 
|
OMP_AN:31394
|

|
OMP:0007033
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increased utilization of inorganic nitrogen source
|
Relative to: OMP_AN:27610

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoF766(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:NH4Cl
|
ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CHEBI:31206) NH4Cl
|

*chemical concentration= 9.5mm
*normalized fitness= 3.16 
|- 
|
OMP_AN:31395
|

|
OMP:0007886
|
decreased resistance to a metal
|
Relative to: OMP_AN:27580

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoF766(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Nickel stress-Ni(II)Cl2 hexahydrate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:53542) Nickel stress-Ni(II)Cl2 hexahydrate
|

*chemical concentration=  1 mM (LB)
*normalized fitness= -3.41 
|- 
|
OMP_AN:31396
|

|
OMP:0007879
|
decreased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27546

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoF766(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Pyocyanin
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:8653) Pyocyanin
|

*chemical concentration=  10 ug/ml
*normalized fitness= -5.66 
|- 
|
OMP_AN:31397
|

|
OMP:0007249
|
decreased resistance to a chemical
|
Relative to: OMP_AN:27567

<hr>Genotype differences:<br>
+lacZ4787(del)( +nuoF766(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Theophylline anhydrous
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:28177) Theophylline anhydrous
|

*chemical concentration=  100 ug/ml
*normalized fitness= -4.52 

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==Additional Information==


==Links==


==References==
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