View source for OMP ST:1056 ! Escherichia coli K-12 JW3882

__TOC__

==Strain Summary==
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!align=left align='left' bgcolor='#CCCCFF' |Strain Name
||
Escherichia coli K-12 JW3882
|-
!align=left align='left' bgcolor='#CCCCFF' |Synonyms
||
JW3882-1
|-
!align=left align='left' bgcolor='#CCCCFF' |Taxon Information
||
*Pangenome: Escherichia coli
*Subspecies and/or strain: K-12
*NCBI Taxonomy ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
|-
!align=left align='left' bgcolor='#CCCCFF' |Genotype
||
DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 cpxA771(del)::kan
|-
!align=left align='left' bgcolor='#CCCCFF' |Strain Reference
||
[http://cgsc.biology.yale.edu/Strain.php?ID=109080 Coli Genetic Stock Center]
|-
!align=left align='left' bgcolor='#CCCCFF' |Strain availability
||
[http://cgsc.biology.yale.edu/Strain.php?ID=109080 Coli Genetic Stock Center]
|-
!align=left align='left' bgcolor='#CCCCFF' |Ancestry
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parent:OMP ST:800 ! Escherichia coli K-12 BW25113
|-
!align=left align='left' bgcolor='#CCCCFF' |Annotated phenotypes
||


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==Notes==


==Phenotype Annotation==
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!|Annotation ID!!Qualifier!!OMP ID!!OMP term name!!Relative phenotype information!!Experimental condition!!ECO ID!!ECO term name!!Reference!!Annotation Extension!!Notes
|- 
|
OMP_AN:968
|

|
OMP:0007092
|
decreased cell motility by swarming
|
Relative to: OMP_AN:881

<hr>Genotype differences:<br>
+DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +cpxA771(del)  -IN(rrnD-rrnE)1
<hr>Condition differences:<br>
no differences
|
*temperature:33C
*medium:swarming motility medium
|
ECO:0005803
|
motility assay evidence
|
PMID:17122336
|

|
 
|- 
|
OMP_AN:34554
|

|
OMP:0007960
|
decreased population growth at low temperature
|
Relative to: OMP_AN:27620

<hr>Genotype differences:<br>
+lacZ4787(del)( +cpxA771(del)  -lacZ4787(del)
<hr>Condition differences:<br>

*temperature:20C
*medium37C
*medium
|

*temperature:20C
*medium:LB agar
*medium:Low temperature
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|

|

*chemical concentration=  20 C
*normalized fitness= -6.37 
|- 
|
OMP_AN:34555
|

|
OMP:0007878
|
increased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27557

<hr>Genotype differences:<br>
+lacZ4787(del)( +cpxA771(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:A22
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:2830968) A22
|

*chemical concentration=  5 ug/ml
*normalized fitness= 3.72 
|- 
|
OMP_AN:34556
|

|
OMP:0007249
|
decreased resistance to a chemical
|
Relative to: OMP_AN:27575

<hr>Genotype differences:<br>
+lacZ4787(del)( +cpxA771(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Acriflavine (acriflavinium chloride)
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:74728) Acriflavine (acriflavinium chloride)
|

*chemical concentration= 2 ug/ml
*normalized fitness= -3.89 
|- 
|
OMP_AN:34557
|

|
OMP:0007927
|
decreased anaerobic population growth
|
Relative to: OMP_AN:27618

<hr>Genotype differences:<br>
+lacZ4787(del)( +cpxA771(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Anaerobic
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|

|

*
*normalized fitness= -4.09 
|- 
|
OMP_AN:34558
|

|
OMP:0007250
|
increased resistance to a chemical
|
Relative to: OMP_AN:27514

<hr>Genotype differences:<br>
+lacZ4787(del)( +cpxA771(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Chlorpromazine hydrochloride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:3649) Chlorpromazine hydrochloride
|

*chemical concentration=  6 uM
*normalized fitness= 3.21 
|- 
|
OMP_AN:34559
|

|
OMP:0007886
|
decreased resistance to a metal
|
Relative to: OMP_AN:27579

<hr>Genotype differences:<br>
+lacZ4787(del)( +cpxA771(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Copper stress-CuCl2 dihydrate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:86318) Copper stress-CuCl2 dihydrate
|

*chemical concentration=  4 mM (LB)
*normalized fitness= -3.52 
|- 
|
OMP_AN:34560
|

|
OMP:0007879
|
decreased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27550

<hr>Genotype differences:<br>
+lacZ4787(del)( +cpxA771(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Ethidium Bromide
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:4883) Ethidium Bromide
|

*chemical concentration=  50 ug/ml
*normalized fitness= -4.9 
|- 
|
OMP_AN:34561
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27603

<hr>Genotype differences:<br>
+lacZ4787(del)( +cpxA771(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-Glucose anhydrous
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:42758) D-Glucose anhydrous
|

*chemical concentration= 0.2%
*normalized fitness= -4.84 
|- 
|
OMP_AN:34562
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27613

<hr>Genotype differences:<br>
+lacZ4787(del)( +cpxA771(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-Glucose anhydrous
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:42758) D-Glucose anhydrous
|

*chemical concentration= 0.2%
*normalized fitness= -4.84 
|- 
|
OMP_AN:34563
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27600

<hr>Genotype differences:<br>
+lacZ4787(del)( +cpxA771(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:NH4Cl
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:31206) NH4Cl
|

*chemical concentration= 9.5mm
*normalized fitness= -3.28 
|- 
|
OMP_AN:34564
|

|
OMP:0007032
|
decreased utilization of inorganic nitrogen source
|
Relative to: OMP_AN:27610

<hr>Genotype differences:<br>
+lacZ4787(del)( +cpxA771(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:NH4Cl
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:31206) NH4Cl
|

*chemical concentration= 9.5mm
*normalized fitness= -3.28 
|- 
|
OMP_AN:34565
|

|
OMP:0007886
|
decreased resistance to a metal
|
Relative to: OMP_AN:27580

<hr>Genotype differences:<br>
+lacZ4787(del)( +cpxA771(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Nickel stress-Ni(II)Cl2 hexahydrate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:53542) Nickel stress-Ni(II)Cl2 hexahydrate
|

*chemical concentration=  1 mM (LB)
*normalized fitness= -4.34 
|- 
|
OMP_AN:34566
|

|
OMP:0007249
|
decreased resistance to a chemical
|
Relative to: OMP_AN:27593

<hr>Genotype differences:<br>
+lacZ4787(del)( +cpxA771(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:(-)-Norepinephrine
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:18357) (-)-Norepinephrine
|

*chemical concentration=  1000 uM
*normalized fitness= -6.17 
|- 
|
OMP_AN:34567
|

|
OMP:0007249
|
decreased resistance to a chemical
|
Relative to: OMP_AN:27573

<hr>Genotype differences:<br>
+lacZ4787(del)( +cpxA771(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Procaine hydrochloride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:8431) Procaine hydrochloride
|

*chemical concentration=  10 mM
*normalized fitness= -5.44 
|- 
|
OMP_AN:34568
|

|
OMP:0006057
|
decreased resistance to aminoglycoside
|
Relative to: OMP_AN:27529

<hr>Genotype differences:<br>
+lacZ4787(del)( +cpxA771(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Tobramycin
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:28864) Tobramycin
|

*chemical concentration= 0.05 ug/ml
*normalized fitness= -3.57 
|- 
|
OMP_AN:34569
|

|
OMP:0007249
|
decreased resistance to a chemical
|
Relative to: OMP_AN:27568

<hr>Genotype differences:<br>
+lacZ4787(del)( +cpxA771(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:(±)-Verapamil hydrochloride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:53188) (±)-Verapamil hydrochloride
|

*chemical concentration=  0.5 mM
*normalized fitness= -5.67 
|- 
|
OMP_AN:34570
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27598

<hr>Genotype differences:<br>
+lacZ4787(del)( +cpxA771(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron excess-FeSO4 heptahydride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride
|

*chemical concentration= 1mM (MOPS; normal 100uM)
*normalized fitness= -6.07 
|- 
|
OMP_AN:34571
|

|
OMP:0007886
|
decreased resistance to a metal
|
Relative to: OMP_AN:27608

<hr>Genotype differences:<br>
+lacZ4787(del)( +cpxA771(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron excess-FeSO4 heptahydride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride
|

*chemical concentration= 1mM (MOPS; normal 100uM)
*normalized fitness= -6.07 
|- 
|
OMP_AN:34572
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27599

<hr>Genotype differences:<br>
+lacZ4787(del)( +cpxA771(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron starvation-FeSO4·7H2O
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O
|

*chemical concentration= 2uM (MOPS; normal 100uM)
*normalized fitness= -4.74 
|- 
|
OMP_AN:34573
|

|
OMP:0007937
|
decreased resistance to nutrient limitation
|
Relative to: OMP_AN:27609

<hr>Genotype differences:<br>
+lacZ4787(del)( +cpxA771(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron starvation-FeSO4·7H2O
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O
|

*chemical concentration= 2uM (MOPS; normal 100uM)
*normalized fitness= -4.74 
|- 
|
OMP_AN:34574
|

|
OMP:0006041
|
increased resistance to quinolone
|
Relative to: OMP_AN:27542

<hr>Genotype differences:<br>
+lacZ4787(del)( +cpxA771(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Norfloxacin
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:100246) Norfloxacin
|

*chemical concentration=  0.02 ug/ml
*normalized fitness= 3.63 
|- 
|
OMP_AN:34575
|

|
OMP:0007833
|
decreased resistance to sulfonamde
|
Relative to: OMP_AN:27576

<hr>Genotype differences:<br>
+lacZ4787(del)( +cpxA771(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Sulfamethizole
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:9331) Sulfamethizole
|

*chemical concentration=  300 ug/ml
*normalized fitness= -3.83 

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==Additional Information==


==Links==


==References==
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