View source for OMP ST:1047 ! Escherichia coli K-12 JW0576

__TOC__

==Strain Summary==
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!align=left align='left' bgcolor='#CCCCFF' |Strain Name
||
Escherichia coli K-12 JW0576
|-
!align=left align='left' bgcolor='#CCCCFF' |Synonyms
||
JW0576-2
|-
!align=left align='left' bgcolor='#CCCCFF' |Taxon Information
||
*Pangenome: Escherichia coli
*Subspecies and/or strain: K-12
*NCBI Taxonomy ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
|-
!align=left align='left' bgcolor='#CCCCFF' |Genotype
||
DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 fes-722(del)::kan
|-
!align=left align='left' bgcolor='#CCCCFF' |Strain Reference
||
[http://cgsc.biology.yale.edu/Strain.php?ID=106976 Coli Genetic Stock Center]
|-
!align=left align='left' bgcolor='#CCCCFF' |Strain availability
||
[http://cgsc.biology.yale.edu/Strain.php?ID=106976 Coli Genetic Stock Center]
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!align=left align='left' bgcolor='#CCCCFF' |Ancestry
||
parent:OMP ST:800 ! Escherichia coli K-12 BW25113
|-
!align=left align='left' bgcolor='#CCCCFF' |Annotated phenotypes
||


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==Notes==


==Phenotype Annotation==
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!|Annotation ID!!Qualifier!!OMP ID!!OMP term name!!Relative phenotype information!!Experimental condition!!ECO ID!!ECO term name!!Reference!!Annotation Extension!!Notes
|- 
|
OMP_AN:959
|

|
OMP:0007092
|
decreased cell motility by swarming
|
Relative to: OMP_AN:881

<hr>Genotype differences:<br>
+DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +fes-722(del)  -IN(rrnD-rrnE)1
<hr>Condition differences:<br>
no differences
|
*temperature:33C
*medium:swarming motility medium
|
ECO:0005803
|
motility assay evidence
|
PMID:17122336
|

|
 
|- 
|
OMP_AN:28722
|

|
OMP:0007249
|
decreased resistance to a chemical
|
Relative to: OMP_AN:27594

<hr>Genotype differences:<br>
+lacZ4787(del)( +fes-722(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:SDS (CHEBI:8984) +EDTA (CHEBI:64758)
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:64758 + CHEBI:8984) SDS (CHEBI:8984) +EDTA (CHEBI:64758)
|

*chemical concentration=  1%/0.5mM
*normalized fitness= -4.71 
|- 
|
OMP_AN:28723
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27601

<hr>Genotype differences:<br>
+lacZ4787(del)( +fes-722(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Sodium Acetate anhydrous
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:32954) Sodium Acetate anhydrous
|

*chemical concentration= 0.6%
*normalized fitness= -6.16 
|- 
|
OMP_AN:28724
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27611

<hr>Genotype differences:<br>
+lacZ4787(del)( +fes-722(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Sodium Acetate anhydrous
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:32954) Sodium Acetate anhydrous
|

*chemical concentration= 0.6%
*normalized fitness= -6.16 
|- 
|
OMP_AN:28725
|

|
OMP:0007879
|
decreased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27548

<hr>Genotype differences:<br>
+lacZ4787(del)( +fes-722(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Cerulenin
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:171741) Cerulenin
|

*chemical concentration=  2 ug/ml
*normalized fitness= -3.88 
|- 
|
OMP_AN:28726
|

|
OMP:0006037
|
increased resistance to macrolide
|
Relative to: OMP_AN:27519

<hr>Genotype differences:<br>
+lacZ4787(del)( +fes-722(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Clarithromycin
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:3732) Clarithromycin
|

*chemical concentration=  1 ug/ml
*normalized fitness= 3.49 
|- 
|
OMP_AN:28727
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27606

<hr>Genotype differences:<br>
+lacZ4787(del)( +fes-722(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:N-Acetyl-D-glucosamine
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:506227) N-Acetyl-D-glucosamine
|

*chemical concentration= 0.15%
*normalized fitness= -3.77 
|- 
|
OMP_AN:28728
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27616

<hr>Genotype differences:<br>
+lacZ4787(del)( +fes-722(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:N-Acetyl-D-glucosamine
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:506227) N-Acetyl-D-glucosamine
|

*chemical concentration= 0.15%
*normalized fitness= -3.77 
|- 
|
OMP_AN:28729
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27602

<hr>Genotype differences:<br>
+lacZ4787(del)( +fes-722(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-(+) Glucosamine hydrochloride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:91431) D-(+) Glucosamine hydrochloride
|

*chemical concentration= 0.2%
*normalized fitness= -5.61 
|- 
|
OMP_AN:28730
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27612

<hr>Genotype differences:<br>
+lacZ4787(del)( +fes-722(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-(+) Glucosamine hydrochloride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:91431) D-(+) Glucosamine hydrochloride
|

*chemical concentration= 0.2%
*normalized fitness= -5.61 
|- 
|
OMP_AN:28731
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27603

<hr>Genotype differences:<br>
+lacZ4787(del)( +fes-722(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-Glucose anhydrous
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:42758) D-Glucose anhydrous
|

*chemical concentration= 0.2%
*normalized fitness= -3.35 
|- 
|
OMP_AN:28732
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27613

<hr>Genotype differences:<br>
+lacZ4787(del)( +fes-722(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-Glucose anhydrous
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:42758) D-Glucose anhydrous
|

*chemical concentration= 0.2%
*normalized fitness= -3.35 
|- 
|
OMP_AN:28733
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27604

<hr>Genotype differences:<br>
+lacZ4787(del)( +fes-722(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Glycerol
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:17754) Glycerol
|

*chemical concentration= 0.4%
*normalized fitness= -4.8 
|- 
|
OMP_AN:28734
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27614

<hr>Genotype differences:<br>
+lacZ4787(del)( +fes-722(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Glycerol
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:17754) Glycerol
|

*chemical concentration= 0.4%
*normalized fitness= -4.8 
|- 
|
OMP_AN:28735
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27605

<hr>Genotype differences:<br>
+lacZ4787(del)( +fes-722(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-(+) Maltose monohydrate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:23615261) D-(+) Maltose monohydrate
|

*chemical concentration= 0.1%
*normalized fitness= -4.18 
|- 
|
OMP_AN:28736
|

|
OMP:0007070
|
decreased organic carbon source utilization
|
Relative to: OMP_AN:27615

<hr>Genotype differences:<br>
+lacZ4787(del)( +fes-722(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:D-(+) Maltose monohydrate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:23615261) D-(+) Maltose monohydrate
|

*chemical concentration= 0.1%
*normalized fitness= -4.18 
|- 
|
OMP_AN:28737
|

|
OMP:0006044
|
decreased resistance to beta-lactam
|
Relative to: OMP_AN:27537

<hr>Genotype differences:<br>
+lacZ4787(del)( +fes-722(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Mecillinam
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:51208) Mecillinam
|

*chemical concentration= 0.03 ug/ml
*normalized fitness= -3.35 
|- 
|
OMP_AN:28738
|

|
OMP:0007879
|
decreased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27547

<hr>Genotype differences:<br>
+lacZ4787(del)( +fes-722(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Hydrogen peroxide
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:16240) Hydrogen peroxide
|

*chemical concentration=  1 mM
*normalized fitness= -3.96 
|- 
|
OMP_AN:28739
|

|
OMP:0007879
|
decreased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27560

<hr>Genotype differences:<br>
+lacZ4787(del)( +fes-722(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Bacitracin Zinc Sulfate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CID:16219032) Bacitracin Zinc Sulfate
|

*chemical concentration=  300 ug/ml
*normalized fitness= -3.36 
|- 
|
OMP_AN:28740
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27599

<hr>Genotype differences:<br>
+lacZ4787(del)( +fes-722(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron starvation-FeSO4·7H2O
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O
|

*chemical concentration= 2uM (MOPS; normal 100uM)
*normalized fitness= -4.62 
|- 
|
OMP_AN:28741
|

|
OMP:0007937
|
decreased resistance to nutrient limitation
|
Relative to: OMP_AN:27609

<hr>Genotype differences:<br>
+lacZ4787(del)( +fes-722(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron starvation-FeSO4·7H2O
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O
|

*chemical concentration= 2uM (MOPS; normal 100uM)
*normalized fitness= -4.62 
|- 
|
OMP_AN:28742
|

|
OMP:0007879
|
decreased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27504

<hr>Genotype differences:<br>
+lacZ4787(del)( +fes-722(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Novobiocin sodium salt
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:31924) Novobiocin sodium salt
|

*chemical concentration=  30 ug/ml
*normalized fitness= -4.02 
|- 
|
OMP_AN:28743
|

|
OMP:0007010
|
decreased population growth at alkaline pH
|
Relative to: OMP_AN:27623

<hr>Genotype differences:<br>
+lacZ4787(del)( +fes-722(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:TAPS
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (**SID:24900259) TAPS
|

*chemical concentration= pH 8 
*normalized fitness= -4.01 
|- 
|
OMP_AN:28744
|

|
OMP:0007008
|
decreased population growth at acidic pH
|
Relative to: OMP_AN:27622

<hr>Genotype differences:<br>
+lacZ4787(del)( +fes-722(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:MES-HOMOPIPES
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (**SID:329817955 SID:57651422) MES-HOMOPIPES
|

*chemical concentration=  6
*normalized fitness= -6.53 

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==Additional Information==


==Links==


==References==
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