View source for OMP ST:1038 ! Escherichia coli K-12 JW0727

__TOC__

==Strain Summary==
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!align=left align='left' bgcolor='#CCCCFF' |Strain Name
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Escherichia coli K-12 JW0727
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!align=left align='left' bgcolor='#CCCCFF' |Synonyms
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JW0727-1
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!align=left align='left' bgcolor='#CCCCFF' |Taxon Information
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*Pangenome: Escherichia coli
*Subspecies and/or strain: K-12
*NCBI Taxonomy ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
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!align=left align='left' bgcolor='#CCCCFF' |Genotype
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DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 tolQ786(del)::kan
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!align=left align='left' bgcolor='#CCCCFF' |Strain Reference
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[http://cgsc.biology.yale.edu/Strain.php?ID=107074 Coli Genetic Stock Center]
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!align=left align='left' bgcolor='#CCCCFF' |Strain availability
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[http://cgsc.biology.yale.edu/Strain.php?ID=107074 Coli Genetic Stock Center]
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!align=left align='left' bgcolor='#CCCCFF' |Ancestry
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parent:OMP ST:800 ! Escherichia coli K-12 BW25113
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!align=left align='left' bgcolor='#CCCCFF' |Annotated phenotypes
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==Notes==


==Phenotype Annotation==
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!|Annotation ID!!Qualifier!!OMP ID!!OMP term name!!Relative phenotype information!!Experimental condition!!ECO ID!!ECO term name!!Reference!!Annotation Extension!!Notes
|- 
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OMP_AN:950
|

|
OMP:0007092
|
decreased cell motility by swarming
|
Relative to: OMP_AN:881

<hr>Genotype differences:<br>
+DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +tolQ786(del)  -IN(rrnD-rrnE)1
<hr>Condition differences:<br>
no differences
|
*temperature:33C
*medium:swarming motility medium
|
ECO:0005803
|
motility assay evidence
|
PMID:17122336
|

|
 
|- 
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OMP_AN:29147
|

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OMP:0007954
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decreased population growth at high temperature
|
Relative to: OMP_AN:27621

<hr>Genotype differences:<br>
+lacZ4787(del)( +tolQ786(del)  -lacZ4787(del)
<hr>Condition differences:<br>

*temperature:45C
*medium37C
*medium
|

*temperature:45C
*medium:LB agar
*medium:High temperature
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
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PMID:21185072
|

|

*chemical concentration=  45 C
*normalized fitness= -4.72 
|- 
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OMP_AN:29148
|

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OMP:0006036
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decreased resistance to macrolide
|
Relative to: OMP_AN:27518

<hr>Genotype differences:<br>
+lacZ4787(del)( +tolQ786(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Azithromycin
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ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:2955) Azithromycin
|

*chemical concentration=  1 ug/ml
*normalized fitness= -4.46 
|- 
|
OMP_AN:29149
|

|
OMP:0007903
|
decreased resistance to bile acids
|
Relative to: OMP_AN:27587

<hr>Genotype differences:<br>
+lacZ4787(del)( +tolQ786(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)
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ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:26711 + CHEBI:9177) Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)
|

*chemical concentration=  1 %
*normalized fitness= -16.48 
|- 
|
OMP_AN:29150
|

|
OMP:0007878
|
increased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27549

<hr>Genotype differences:<br>
+lacZ4787(del)( +tolQ786(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:CHIR-090
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CHEBI:134107 CID:11546620) CHIR-090
|

*chemical concentration=  0.025 ug/ml
*normalized fitness= 3.68 
|- 
|
OMP_AN:29151
|

|
OMP:0006045
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increased resistance to beta-lactam compound
|
Relative to: OMP_AN:27534

<hr>Genotype differences:<br>
+lacZ4787(del)( +tolQ786(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Cefoxitin sodium hydrate
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CHEBI:3501) Cefoxitin sodium hydrate
|

*chemical concentration=  0.75 ug/ml
*normalized fitness= 3.89 
|- 
|
OMP_AN:29152
|

|
OMP:0007903
|
decreased resistance to bile acids
|
Relative to: OMP_AN:27585

<hr>Genotype differences:<br>
+lacZ4787(del)( +tolQ786(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Cholate sodium hydrate
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
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PMID:21185072
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towards some (CID:23679061) Cholate sodium hydrate
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*chemical concentration=  0.5 %
*normalized fitness= -7.24 
|- 
|
OMP_AN:29153
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OMP:0006037
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increased resistance to macrolide
|
Relative to: OMP_AN:27519

<hr>Genotype differences:<br>
+lacZ4787(del)( +tolQ786(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Clarithromycin
|
ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CHEBI:3732) Clarithromycin
|

*chemical concentration= 0.1 ug/ml
*normalized fitness= 4.87 
|- 
|
OMP_AN:29154
|

|
OMP:0007886
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decreased resistance to a metal
|
Relative to: OMP_AN:27579

<hr>Genotype differences:<br>
+lacZ4787(del)( +tolQ786(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Copper stress-CuCl2 dihydrate
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CHEBI:86318) Copper stress-CuCl2 dihydrate
|

*chemical concentration=  4 mM (LB)
*normalized fitness= -11.29 
|- 
|
OMP_AN:29155
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OMP:0006061
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decreased resistance to tetracycline
|
Relative to: OMP_AN:27523

<hr>Genotype differences:<br>
+lacZ4787(del)( +tolQ786(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Minocycline hydrochloride
|
ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CHEBI:50697) Minocycline hydrochloride
|

*chemical concentration=  1 ug/ml
*normalized fitness= -4.39 
|- 
|
OMP_AN:29156
|

|
OMP:0007903
|
decreased resistance to bile acids
|
Relative to: OMP_AN:27584

<hr>Genotype differences:<br>
+lacZ4787(del)( +tolQ786(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Taurocholic acid sodium salt hydrate
|
ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CID:23687511) Taurocholic acid sodium salt hydrate
|

*chemical concentration=  1 %
*normalized fitness= -4.21 
|- 
|
OMP_AN:29157
|

|
OMP:0007879
|
decreased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27588

<hr>Genotype differences:<br>
+lacZ4787(del)( +tolQ786(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Triclosan/Irgasan
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CHEBI:164200) Triclosan/Irgasan
|

*chemical concentration= 0.05 ug/ml
*normalized fitness= -7.76 
|- 
|
OMP_AN:29158
|

|
OMP:0007896
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decreased resistance to a surfactant
|
Relative to: OMP_AN:27583

<hr>Genotype differences:<br>
+lacZ4787(del)( +tolQ786(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Triton X-100
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CHEBI:9750) Triton X-100
|

*chemical concentration=  0.03 %
*normalized fitness= -5.5 
|- 
|
OMP_AN:29159
|

|
OMP:0007879
|
decreased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27589

<hr>Genotype differences:<br>
+lacZ4787(del)( +tolQ786(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Cefsulodin + Mecillinam
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CHEBI:31380 + CHEBI:51208) Cefsulodin + Mecillinam
|

*chemical concentration= 6 + 0.03 ug/ml
*normalized fitness= -11.72 
|- 
|
OMP_AN:29160
|

|
OMP:0006061
|
decreased resistance to tetracycline
|
Relative to: OMP_AN:27522

<hr>Genotype differences:<br>
+lacZ4787(del)( +tolQ786(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Doxycycline hyclate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:34730) Doxycycline hyclate
|

*chemical concentration=  1 ug/ml
*normalized fitness= -4.26 
|- 
|
OMP_AN:29161
|

|
OMP:0006044
|
decreased resistance to beta-lactam
|
Relative to: OMP_AN:27538

<hr>Genotype differences:<br>
+lacZ4787(del)( +tolQ786(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Oxacillin sodium salt
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:52134) Oxacillin sodium salt
|

*chemical concentration=  5 ug/ml
*normalized fitness= -5.91 
|- 
|
OMP_AN:29162
|

|
OMP:0007011
|
increased population growth at alkaline pH
|
Relative to: OMP_AN:27623

<hr>Genotype differences:<br>
+lacZ4787(del)( +tolQ786(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:TAPS
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (**SID:24900259) TAPS
|

*chemical concentration=  10
*normalized fitness= 4.7 
|- 
|
OMP_AN:29163
|

|
OMP:0007878
|
increased resistance to an antimicrobial agent
|
Relative to: OMP_AN:27565

<hr>Genotype differences:<br>
+lacZ4787(del)( +tolQ786(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Polymyxin B sulfate
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:8310) Polymyxin B sulfate
|

*chemical concentration=  6 ug/ml
*normalized fitness= 4.28 

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==Additional Information==


==Links==


==References==
{{RefHelp}}<references/>