View source for PMID:21541325

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!align=left  |Citation
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'''Deininger, KN, Horikawa, A, Kitko, RD, Tatsumi, R, Rosner, JL, Wachi, M and Slonczewski, JL'''  (2011) A requirement of TolC and MDR efflux pumps for acid adaptation and GadAB induction in Escherichia coli.''PLoS ONE'' '''6''':e18960
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!align=left  |Abstract
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The TolC outer membrane channel is a key component of several multidrug resistance (MDR) efflux pumps driven by H(+) transport in Escherichia coli. While tolC expression is under the regulation of the EvgA-Gad acid resistance regulon, the role of TolC in growth at low pH and extreme-acid survival is unknown.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=21541325 PubMed]
Online version:[http://dx.doi.org/10.1371/journal.pone.0018960 10.1371/journal.pone.0018960]
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!align=left  |Keywords
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Acids; Adaptation, Physiological; Bacterial Outer Membrane Proteins; Drug Resistance, Multiple; Enzyme Induction; Escherichia coli K12; Escherichia coli Proteins; Glutamate Decarboxylase; Glutamates; Hydrogen-Ion Concentration; Membrane Proteins; Membrane Transport Proteins; Microbial Viability; Mutation

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==Main Points of the Paper ==
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== Materials and Methods Used ==
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==Phenotype Annotations==
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
*Strain: K-12
*Substrain: JLS1015
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
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*Genotype of Reference Strain: tolC
*Genotype of Experimental Strain : ''tolC732::Kan''
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*Reference Condition:
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TolC was required for EXTREME-acid survival (pH 2) of strain W3110 grown aerobically to stationary phase. A tolC deletion decreased extreme-acid survival (acid resistance) of aerated pH 7.0-grown cells by 105-fold and of pH 5.5-grown cells by 10-fold. TolC was required for expression of glutamate decarboxylase (GadA, GadB), a key component of glutamate-dependent acid resistance (Gad). TolC was also required for maximal exponential growth of E. coli K-12 W3110, in LBK medium buffered at pH 4.5-6.0, but not at pH 6.5-8.5. The TolC growth requirement in moderate acid was independent of Gad.
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==Notes==

==References==
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