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!align=left  |Citation
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'''Sandler, SJ, Marians, KJ, Zavitz, KH, Coutu, J, Parent, MA and Clark, AJ'''  (1999) dnaC mutations suppress defects in DNA replication- and recombination-associated functions in priB and priC double mutants in Escherichia coli K-12.''Mol. Microbiol.'' '''34''':91-101
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!align=left  |Abstract
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PriA, PriB and PriC were originally discovered as proteins essential for the PhiX174 in vitro DNA replication system. Recent studies have shown that PriA mutants are poorly viable, have high basal levels of SOS expression (SOSH), are recombination deficient (Rec-), sensitive to UV irradiation (UVS) and sensitive to rich media. These data suggest that priA's role may be more complex than previously thought and may involve both DNA replication and homologous recombination. Based on the PhiX174 system, mutations in priB and priC should cause phenotypes like those seen in priA2:kan mutants. To test this, mutations in priB and priC were constructed. We found that, contrary to the PhiX174 model, del(priB)302 and priC303:kan mutants have almost wild-type phenotypes. Most unexpectedly, we then found that the priBC double mutant had very poor viability and/or a slow growth rate (even less than a priA2:kan mutant). This suggests that priB and priC have a redundant and important role in Escherichia coli. The priA2:kan suppressor, dnaC809, partially suppressed the poor viability/slow growth phenotype of the priBC double mutant. The resulting triple mutant (priBC dnaC809 ) had small colony size, recombination deficiency and levels of SOS expression similar to a priA2:kan mutant. The priBC dnaC809 mutant, however, was moderately UVR and had good viability, unlike a priA2:kan mutant. Additional mutations in the triple mutant were selected to suppress the slow growth phenotype. One suppressor restored all phenotypes tested to nearly wild-type levels. This mutation was identified as dnaC820 (K178N) [mapping just downstream of dnaC809 (E176G)]. Experiments suggest that dnaC820 makes dnaC809 suppression of priA and or priBC mutants priB and or priC independent. A model is proposed for the roles of these proteins in terms of restarting collapsed replication forks from recombinational intermediates.
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!align=left  |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10540288 PubMed]

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!align=left  |Keywords
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Bacterial Proteins; Bacteriophage mu; Cell Division; DNA Replication; DNA, Bacterial; DNA-Binding Proteins; Escherichia coli; Escherichia coli Proteins; Mutation; Phenotype; Recombination, Genetic; Replication Protein A; SOS Response (Genetics); Substrate Specificity; Suppression, Genetic

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==Main Points of the Paper ==
{{LitSignificance}}

== Materials and Methods Used ==
{{LitMaterials}}

==Phenotype Annotations==
{{AnnotationTableHelp}}
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="P4f1db649a9061"  class=" tableEdit Phenotype_Table_2"  
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
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|
a mutation or genetic difference within a strain
|
*Taxon: Escherichia coli
*Strain: K-12
*Substrain: JC19323
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
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*Genotype of Reference Strain: ''dnaC''
*Genotype of Experimental Strain : ''dnaC820''
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*Reference Condition:
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''dnaC820'' nullify double mutant ''priB'' ''priC'' which restores growth and viability to WT levels. figure 1.
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Delete 
|- 
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a mutation or genetic difference within a strain
|
*Taxon: Escherichia Coli
*Strain: K-12
*Substrain: JC19008
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
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*Genotype of Reference Strain: ''dnaC''
*Genotype of Experimental Strain : ''dnaC809''
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*Reference Condition:
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|

|

|

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This mutation will suppress the inviability of the single mutation priA2::kan, table 1.
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Delete 
|- 
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a mutation or genetic difference within a strain
|
*Taxon: Escherichia coli
*Strain: K-12
*Substrain: DM4000
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
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*Genotype of Reference Strain: priB+
*Genotype of Experimental Strain : priB302(del)
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*Reference Condition:
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OMP:0005056
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decreased frequency of site-specific recombination
|

|

|
Reference Table 1;
P1 Transduction evidence
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needs eco and ref condition 
|- 
|
a mutation or genetic difference within a strain
|
*Taxon: Escherichia coli
*Strain: K-12
*Substrain: DM4000
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
|
*Genotype of Reference Strain: priB+
*Genotype of Experimental Strain : priB302(del)
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*Reference Condition: 20 J UV compared to 0 J
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OMP:0005084
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UV radiation resistance phenotype
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ECO:0000095
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cell growth assay evidence
|
Reference Table 1;
phenotype is unchanged
|
 
|- 
|
a mutation or genetic difference within a strain
|
*Taxon: Escherichia coli
*Strain: K-12
*Substrain: DM4000
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
|
*Genotype of Reference Strain: priB+
*Genotype of Experimental Strain : priB302(del)
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*Reference Condition: UV-irradiation at .5 J/m^2/sec for 10 seconds
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OMP:0005023
|
presence of an SOS response
|
ECO:0000297
|
LacZ transcript localization evidence
|
Reference Table 1;
phenotype is unchanged
|
 
|- 
|
a mutation or genetic difference within a strain
|
*Taxon: Escherichia coli
*Strain: K-12
*Substrain: DM4000
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
|
*Genotype of Reference Strain: priC+
*Genotype of Experimental Strain : priC303::kan
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*Reference Condition:
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OMP:0005058
|
presence of site-specific recombination
|

|

|
Reference Table 1;
P1 Transduction evidence
|
needs eco and ref condition 
|- 
|
a mutation or genetic difference within a strain
|
*Taxon: Escherichia coli
*Strain: K-12
*Substrain: DM4000
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
|
*Genotype of Reference Strain: priC+
*Genotype of Experimental Strain : priC303::kan
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*Reference Condition: 20 J UV compared to 0 J
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OMP:0005084
|
UV radiation resistance phenotype
|
ECO:0000095
|
cell growth assay evidence
|
Reference Table 1;
phenotype is unchanged
|
 
|- 
|
a mutation or genetic difference within a strain
|
*Taxon: Escherichia coli
*Strain: K-12
*Substrain: DM4000
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
|
*Genotype of Reference Strain: priC+
*Genotype of Experimental Strain : priC303::kan
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*Reference Condition: UV-irradiation at .5 J/m^2/sec for 10 seconds
|
OMP:0005023
|
presence of an SOS response
|
ECO:0000297
|
LacZ transcript localization evidence
|
Reference Table 1;
phenotype is unchanged
|
 
|- 
|
a mutation or genetic difference within a strain
|
*Taxon: Escherichia coli
*Strain: K-12
*Substrain: DM4000
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
|
*Genotype of Reference Strain: priA+
*Genotype of Experimental Strain : priA2::kan
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*Reference Condition:
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OMP:0005056
|
decreased frequency of site-specific recombination
|

|

|
Reference Table 1;
P1 Transduction evidence
|
needs eco and ref condition 
|- 
|
a mutation or genetic difference within a strain
|
*Taxon: Escherichia coli
*Strain: K-12
*Substrain: DM4000
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
|
*Genotype of Reference Strain: priA+
*Genotype of Experimental Strain : priA2::kan
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*Reference Condition: 20 J UV compared to 0 J
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OMP:0005098
|
decreased resistance to UV radiation
|
ECO:0000095
|
cell growth assay evidence
|
Reference Table 1
|
 
|- 
|
a mutation or genetic difference within a strain
|
*Taxon: Escherichia coli
*Strain: K-12
*Substrain: DM4000
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
|
*Genotype of Reference Strain: priA+
*Genotype of Experimental Strain : priA2::kan
|
*Reference Condition: UV-irradiation at .5 J/m^2/sec for 10 seconds
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OMP:0005025
|
increased induced expression of the SOS response
|
ECO:0000297
|
LacZ transcript localization evidence
|
Reference Table 1
|
 
|- 
|
a mutation or genetic difference within a strain
|
*Taxon: Escherichia coli
*Strain: K-12
*Substrain: DM4000
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
|
*Genotype of Reference Strain: priB+ priC+
*Genotype of Experimental Strain : priB302(del) priC303::kan
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*Reference Condition: minimal media containing 50 micrograms ml^-1 kanamycin, 15 micrograms ml^-1 X-gal, incubation 3-4 days
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OMP:0005020
|
increased basal expression of the SOS response
|
ECO:0000297
|
lacZ transcript localization evidence
|
Reference section 2 of results section
|
 
|- 
|
a mutation or genetic difference within a strain
|
*Taxon: Escherichia coli
*Strain: K-12
*Substrain: DM4000
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
|
*Genotype of Reference Strain: priB+ priC+ dnaC+
*Genotype of Experimental Strain : priB302(del) priC303::kan dnaC809
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*Reference Condition:
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OMP:0005056
|
decreased frequency of site-specific recombination
|

|

|
Reference Table 1;
P1 Transduction evidence
|
needs eco and ref condition 
|- 
|
a mutation or genetic difference within a strain
|
*Taxon: Escherichia coli
*Strain: K-12
*Substrain: DM4000
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
|
*Genotype of Reference Strain: priB+ priC+ dnaC+
*Genotype of Experimental Strain : priB302(del) priC303::kan dnaC809
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*Reference Condition: 20 J UV compared to 0 J
|
OMP:0005098
|
decreased resistance to UV radiation
|
ECO:0000095
|
cell growth assay evidence
|
Reference Table 1; phenotype is unchanged
|
 
|- 
|
a mutation or genetic difference within a strain
|
*Taxon: Escherichia coli
*Strain: K-12
*Substrain: DM4000
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
|
*Genotype of Reference Strain: priB+ priC+ dnaC+
*Genotype of Experimental Strain : priB302(del) priC303::kan dnaC809
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*Reference Condition: UV-irradiation at .5 J/m^2/sec for 10 seconds
|
OMP:0005020
|
increased basal expression of the SOS response
|
ECO:0000297
|
LacZ transcript localization evidence
|
Reference Table 1; 
phenotype is unchanged

|
 
|- 
|
a mutation or genetic difference within a strain
|
*Taxon: Eschericia coli
*Strain: K-12
*Substrain: DM4000
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
|
*Genotype of Reference Strain: priB+ priC+ dnaC+
*Genotype of Experimental Strain : priB302(del) priC303::kan dnaC809,820
|
*Reference Condition: 20 J UV compared to 0 J
|
OMP:0005084
|
UV radiation resistance phenotype
|
ECO:0000095
|
Cell growth assay evidence
|
Reference Table 1;
Phenotype is unchanged
|
 
|- 
|
a mutation or genetic difference within a strain
|
*Taxon: Eschericia coli
*Strain: K-12
*Substrain: DM4000
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
|
*Genotype of Reference Strain: priB+ priC+ dnaC+
*Genotype of Experimental Strain : priB302(del) priC303::kan dnaC809,820
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*Reference Condition: UV-irradiation at .5 J/m^2/sec for 10 seconds
|
OMP:0005023 
|
presence of an SOS response
|
ECO:0000297
|
lacZ transcript localization evidence
|
Reference Table 1;
Phenotype is unchanged
|
 
|- 
|
a mutation or genetic difference within a strain
|
*Taxon: Eschericia coli
*Strain: K-12
*Substrain: DM4000
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
|
*Genotype of Reference Strain: priB+ priC+ dnaC+
*Genotype of Experimental Strain : priB302(del) priC303::kan dnaC809,820
|
*Reference Condition:
|

|

|

|

|

|
 
|- 
|
a mutation or genetic difference within a strain
|
*Taxon: Escherichia coli
*Strain: K-12
*Substrain: DM4000
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
|
*Genotype of Reference Strain: priA+ priB+ priC+ dnaC+
*Genotype of Experimental Strain : priA::kan priB302(del) priC303::kan dnaC809
|
*Reference Condition: 20 J UV compared to 0 J
|
OMP:0005098
|
decreased resistance to UV radiation
|
ECO:0000095
|
cell growth assay evidence
|
Reference Table 1;
Evidence that PriA and DnaC809 do not compete for the same substrate
|
 
|- 
|
a mutation or genetic difference within a strain
|
*Taxon: Escherichia coli
*Strain: K-12
*Substrain: DM4000
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
|
*Genotype of Reference Strain: priA+ priB+ priC+ dnaC+
*Genotype of Experimental Strain : priA::kan priB302(del) priC303::kan dnaC809
|
*Reference Condition: UV-irradiation at .5 J/m^2/sec for 10 seconds
|
OMP:0005020
|
increased basal expression of the SOS response
|
ECO:0000297
|
lacZ transcript localization evidence
|
Reference Table 1; Evidence that PriA and DnaC809 do not compete for the same substrate
|
 
|- 
|
a mutation or genetic difference within a strain
|
*Taxon: Escherichia coli
*Strain: K-12
*Substrain: DM4000
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
|
*Genotype of Reference Strain: priA+ priB+ priC+ dnaC+
*Genotype of Experimental Strain : priA::kan priB302(del) priC303::kan dnaC809
|
*Reference Condition:
|
OMP:0005056
|
decreased frequency of site-specific recombination
|

|

|
Reference Table 1; 
Evidence that PriA and DnaC809 do not compete for the same substrate
|
Needs eco and ref condition 

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==Notes==

==References==
{{RefHelp}}
<references/>


[[Category:Publication]]