View source for PMID:7984093

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!align=left  |Citation
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'''Tsui, HC, Leung, HC and Winkler, ME'''  (1994) Characterization of broadly pleiotropic phenotypes caused by an hfq insertion mutation in Escherichia coli K-12.''Mol. Microbiol.'' '''13''':35-49
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!align=left  |Abstract
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The region immediately downstream from the miaA tRNA modification gene at 94.8 min contains the hfq gene and the hflA region, which are important in the bacteriophage Q beta and lambda life cycles. The roles of these genes in bacteria remain largely unknown. We report here the characterization of two chromosomal hfq insertion mutations. An omega (omega) cassette insertion near the end of hfq resulted in phenotypes only slightly different from the parent, although transcript mapping demonstrated that the insertion was completely polar on hflX expression. In contrast, an equally polar omega cassette insertion near the beginning of hfq caused pronounced pleiotropic phenotypes, including decreased growth rates and yields, decreased negative supercoiling of plasmids in stationary phase, increased cell size, osmosensitivity, increased oxidation of carbon sources, increased sensitivity to ultraviolet light, and suppression of bgl activation by hns mutations. hfq::omega mutant phenotypes were distinct from those caused by omega insertions early in the miaA tRNA modification gene. On the other hand, both hfq insertions interfered with lambda phage plaque formation, probably by means of polarity at the hflA region. Together, these results show that hfq function plays a fundamental role in Escherichia coli physiology and that hfq and the hflA-region are in the amiB-mutL-miaA-hfq-hflX superoperon.
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!align=left  |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7984093 PubMed]

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!align=left  |Keywords
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Alkyl and Aryl Transferases; Bacterial Proteins; Bacteriophage lambda; Base Sequence; Carrier Proteins; Chromosome Mapping; Coliphages; DNA, Recombinant; Escherichia coli; Escherichia coli Proteins; GTP-Binding Proteins; Gene Expression Regulation, Bacterial; Host Factor 1 Protein; Integration Host Factors; Membrane Proteins; Molecular Sequence Data; Mutagenesis, Insertional; Operon; Phenotype; Serine Endopeptidases; Transcription, Genetic; Transferases; Virus Replication

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==Main Points of the Paper ==
{{LitSignificance}}

== Materials and Methods Used ==
{{LitMaterials}}

==Phenotype Annotations==
{{AnnotationTableHelp}}
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="Z4e67ac8edde08"  class=" tableEdit Phenotype_Table_2"  
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
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a mutation or genetic difference within a strain
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*Taxon: Esch
*Strain: K-12
*Substrain: MG1655
*NCBI Taxon ID: 51145
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*Genotype of Reference Strain: ''hfq+''
*Genotype of Experimental Strain : ''hfq1::omega''
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*Reference Condition:
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0000280
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increased cell length
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0000182
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in vitro culture assay
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The mutation was an insertion of a omega cassette near the start of hfq sequence of this strain. Causing change in cell shape, specifically, elongation of the cells. 
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
*Strain: K-12
*Substrain: MG1655
*NCBI Taxon ID: 511145
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*Genotype of Reference Strain: ''hfq+''
*Genotype of Experimental Strain : "'hfq::omega''
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*Reference Condition:
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0000283
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decreased growth rate
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There was an Insertion of a omega cassette near the start of hfq sequence of the strain, causing a decrease in the strains growth rate and overall yield. 
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
*Strain: MG1655
*Substrain: 51145
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*Genotype of Reference Strain: ''hfq+''
*Genotype of Experimental Strain : "'hfq::omega''
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*Reference Condition:
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0001002
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decreased negative DNA supercoiling
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An omega cassette was inserted towards the start of the ''hfq'' sequence causing a change in cell shape, specifically a relaxation of the supercoiling. 
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a single strain under different conditions
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*Taxon: Escherichia coli
*Strain: K-12
*Substrain: MG1655
*NCBI Taxon ID: 51145
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*Genotype of Reference Strain: ''hfq+''
*Genotype of Experimental Strain : "'hfq::omega''
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*Reference Condition:
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0005068
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increased mutation frequency
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An omega cassette was inserted near the start of the ''hfq'' sequence causing spontaneous mutagenesis. 
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
*Strain: MG1655
*Substrain: 511145
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*Genotype of Reference Strain: ''hfq+''
*Genotype of Experimental Strain : ''hfq1::omega''
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*Reference Condition:
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0005098
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decreased resistance to UV radiation
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The mutation was the insertion of a omega cassette near the beginning of the ''hfq'' sequence of the strain causing a sensitivity to Ultraviolet light. 
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
*Strain: MG1655
*Substrain: 511145
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*Genotype of Reference Strain: ''hfq+''
*Genotype of Experimental Strain : "'hfq::omega''
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*Reference Condition:
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0007071
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increased carbon source utilization
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The insertion of an omega cassette near the start of the ''hfq'' sequence caused an increase in the  oxidation of various carbon sources. 
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
*Strain: MG1655
*Substrain: 511145
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*Genotype of Reference Strain: ''hfq+''
*Genotype of Experimental Strain : ''hfq1::omega''
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*Reference Condition:
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This mutation involved the insertion of a omega cassette near the end of the ''hfq'' sequence. It resulted in no substantial difference from ''hfq+'' parent strain. 
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==Notes==

==References==
{{RefHelp}}
<references/>


[[Category:Publication]]