PMID:8509326

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Citation

Furukawa, H, Tsay, JT, Jackowski, S, Takamura, Y and Rock, CO (1993) Thiolactomycin resistance in Escherichia coli is associated with the multidrug resistance efflux pump encoded by emrAB.J. Bacteriol. 175:3723-9

Abstract

Thiolactomycin (TLM) and cerulenin are antibiotics that block Escherichia coli growth by inhibiting fatty acid biosynthesis at the beta-ketoacyl-acyl carrier protein synthase I step. Both TLM and cerulenin trigger the accumulation of intracellular malonyl-coenzyme A coincident with growth inhibition, and the overexpression of synthase I protein confers resistance to both antibiotics. Strain CDM5 was derived as a TLM-resistant mutant but remained sensitive to cerulenin. TLM neither induced malonyl-coenzyme A accumulation nor blocked fatty acid production in vivo; however, the fatty acid synthase activity in extracts from strain CDM5 was sensitive to TLM inhibition. The TLM resistance gene in strain CDM5 was mapped to 57.5 min of the chromosome and was an allele of the emrB gene. Disruption of the emrB gene converted strain CDM5 to a TLM-sensitive strain, and the overexpression of the emrAB operon conferred TLM resistance to sensitive strains. Thus, activation of the emr efflux pump is the mechanism for TLM resistance in strain CDM5.

Links

PubMed

Keywords

Biological Transport; Cerulenin; Chromosome Mapping; Drug Resistance, Microbial; Escherichia coli; Fatty Acid Synthetase Complex; Genes, Bacterial; Malonyl Coenzyme A; Operon; Thiophenes

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escerichia coli
  • Strain: K-12
  • Substrain:
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: emrB
  • Genotype of Experimental Strain : emrB in mutant strain CDM5
  • Reference Condition:
OMP:0006058

increased resistance to aminoglycoside compound

Resistant to Thiolactomycin in vivo. It is still sensitive in Vitro.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Genotype of Reference Strain: nalB
  • Genotype of Experimental Strain : nalB4
  • Reference Condition:
OMP:0006058

increased resistance to aminoglycoside compound

Resistance to Thiolactomycin.


Notes

ECOID: Solid minimal medium in VIVO (in vitro - is not resistant).

OMP:

References

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