PMID:780339

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Citation

White, BJ, Hochhauser, SJ, Cintron, NM and Weiss, B (1976) Genetic mapping of xthA, the structural gene for exonuclease III in Escherichia coli K-12.J. Bacteriol. 126:1082-8

Abstract

The genes xthA, pncA, and pabB were ordered relative to others by two- and three-factor transductional crosses with bacteriophage P1. The genes studied span 2 min (2%) of the genetic map of Escherichia coli K-12 in the clockwise sequence pheS-pfkB-xthA-pncA-gap-pabB-fadD. Eleven independently derived xth mutations were examined; all were known to affect exonuclease III and its associated endonuclease II activity, and all were mapped in the xthA region. pncA mutations were found to confer resistance to 6-aminonicotinamide, whereas some pheS mutations are known to specify resistance to p-fluorphenylalanine. xth mutations were readily transferred into other strains by selecting for these co-transducible drug resistance markers.

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Keywords

6-Aminonicotinamide; Chromosome Mapping; Deoxyribonucleases; Drug Resistance, Microbial; Endonucleases; Escherichia coli; Exonucleases; Genes; Transduction, Genetic; p-Fluorophenylalanine

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain:
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: pncA
  • Genotype of Experimental Strain : pncA
  • Reference Condition:
OMP:0000276

increased resistance to antibiotics

ECO:0000034

non-traceable author statement

Resistant to 6-amino-nicotinamide


Notes

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