PMID:7047497

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Citation

Stewart, V and MacGregor, CH (1982) Nitrate reductase in Escherichia coli K-12: involvement of chlC, chlE, and chlG loci.J. Bacteriol. 151:788-99

Abstract

We examined the properties of mutants of E. coli which are defective with respect to nitrate reductase activity. chlE::Mu cts and chlG::Mu cts mutants were all chlorate resistant, and the strains that we examined all synthesized nitrate reductase apoenzyme. We concluded that the chlE and chlG loci, like the chlA, chlB, and chlD loci, are involved in the synthesis of insertion of molybdenum cofactor. We identified at least four distinct phenotypic classes of chlC::Tn10 mutants, all of which were fully or partially sensitive to chlorate. Two of these classes may represent lesions in the structural genes for nitrate reductase subunits A and C. Two other classes may be altered in the regulation of the expression of nitrate reductase or other anaerobic enzymes. We propose the mnemonic nar for naming individual genes within the chlC locus.

Links

PubMed

Keywords

Apoenzymes; Chlorates; Coenzymes; Escherichia coli; Genes; Genes, Bacterial; Metalloproteins; Molybdenum; Mutation; Nitrate Reductases; Pteridines

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: RK5278
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: nar-215
  • Genotype of Experimental Strain : nar-215::Tn10
  • Reference Condition:

OMP:0000298

enzyme activity phenotype

ECO:0000184

enzyme inhibition experiment data

mutant is narL - and has reduced Nitrate Reductase Activity

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: RK5278
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: nar-215
  • Genotype of Experimental Strain : nar-215::Tn10
  • Reference Condition:

OMP:0000298

enzyme activity phenotype

ECO:0000184

enzyme inhibition experiment data

mutant is narL- and has decreased formate activity

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: RK5266
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: nar-203
  • Genotype of Experimental Strain : nar-203::Tn10
  • Reference Condition:

OMP:0000298

enzyme activity phenotype

ECO:0000039

protein assay data

Mutant is NarK - and has an Incrase in nitrase reductase activity

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: RK5266
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: nar203
  • Genotype of Experimental Strain : nar-203::Tn10
  • Reference Condition:
OMP:0000269

protein activity phenotype

ECO:0000039

protein assay data

Mutant is NarK - and has Reduced Formate activity

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: RK5267
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: nar-204
  • Genotype of Experimental Strain : nar-204::Tn10
  • Reference Condition:
OMP:0000269

protein activity phenotype

ECO:0000039

protein assay data

Mutant is NarI - and has reduced nitrate reductase activity

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: RK5267
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: nar-204
  • Genotype of Experimental Strain : nar-204::Tn10
  • Reference Condition:
ECO:0000039

protein assay data

OMP:0000269

protein activity phenotype

Mutant is NarI - and has reduced Formate activity

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: RK5263
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: nar-200
  • Genotype of Experimental Strain : nar-200::Tn10
  • Reference Condition:
OMP:0000269

protein activity phenotype

ECO:0000039

protein assay data

mutant is NarH - and there is reduced nitrate reductase activity

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: RK5263
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: nar-200
  • Genotype of Experimental Strain : nar-200::Tn10
  • Reference Condition:
OMP:0000269

protein activity phenotype

ECO:0000039

protein assay data

Mutant is NarH - and there is reduced formate activity.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: RK5265
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: nar-202
  • Genotype of Experimental Strain : nar-202::Tn10
  • Reference Condition:
OMP:0000269

protein activity phenotype

ECO:0000039

protein assay data

mutant is narG - and has reduced Nitrate reductase activity (roughly 1% of wildtype activity).

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: RK5265
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: nar-202
  • Genotype of Experimental Strain : nar-202::Tn10
  • Reference Condition:
OMP:0000269

protein activity phenotype

ECO:0000039

protein assay data

Mutant is narG- and has Reduced Formate activity

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain:
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: fnr
  • Genotype of Experimental Strain : fnr-
  • Reference Condition:

OMP:0000270

absence of growth

ECO:0000094

biological assay data

Mutants were sensitive to minimun 5 mM of chlorate.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: RK5230-5233
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: chlG
  • Genotype of Experimental Strain : chlG::Mu cts
  • Reference Condition:

OMP:0000270

absence of growth

ECO:0000094

biological assay data

chlorate resistance

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain:
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: chlG
  • Genotype of Experimental Strain : chlG::Mu cts
  • Reference Condition:

OMP:0000298

enzyme activity phenotype

ECO:0000039

protein assay data

decrease formate hydrogenlyase gas

a mutation or genetic difference within a strain

  • Taxon: Escherichai coli
  • Strain: K-12
  • Substrain:
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: chlG
  • Genotype of Experimental Strain : chlG::Mu cts
  • Reference Condition:
OMP:0000269

protein activity phenotype

ECO:0000039

protein assay data

reduced nitrate reductase activity

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain:
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: chlG
  • Genotype of Experimental Strain : chlG::Mu cts
  • Reference Condition:
OMP:0000255

plating phenotype

ECO:0000039

protein assay data

deficient in the expression of tiary amine oxide reductase activity, as estimated by their phenotypes on MacConkey TMAO medium.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain:
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: clhE
  • Genotype of Experimental Strain : chlE::mu cts
  • Reference Condition:

OMP:0000270

absence of growth

ECO:0000094

biological assay data

Chlorate Resistance

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: RK5201, RK5218, RK5223, RK5228, RK5240, RK5255
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: clhE
  • Genotype of Experimental Strain : chlE::mu cts
  • Reference Condition:
OMP:0000269

protein activity phenotype

ECO:0000039

protein assay data

Inhibition of nitrate reductase activity

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: RK5201, RK5218, RK5223, RK5228, RK5240, RK5255
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: chlE
  • Genotype of Experimental Strain : chlE::mu cts
  • Reference Condition:
ECO:0000039

protein assay data

OMP:0000269

protein activity phenotype

Inhibition of formate hydrogenlyase (formate dehydrogenase plus hydrogenase) thus failing to produce gas.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: RK5201, RK5218, RK5223, RK5228, RK5240, RK5255
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: chlE
  • Genotype of Experimental Strain : chlE::mu cts
  • Reference Condition:
OMP:0000255

plating phenotype

ECO:0000039

protein assay data

Deficient in Tiary amine oxide reductase activity in anaerobic growth on MacConkey TMAO medium.


Notes

The OMP term needs revision when appropriate OMP term is created and approved (in regards to enzyme activity).

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