PMID:6998971

From OMPwiki
Jump to: navigation, search
Citation

Ferenci, T (1980) Methyl-alpha-maltoside and 5-thiomaltose: analogs transported by the Escherichia coli maltose transport system.J. Bacteriol. 144:7-11

Abstract

Neither methyl-alpha-maltoside nor 5-thiomaltose is utilized by Escherichia coli as a sole carbon source. Both are, however, effective competitive inhibitors of maltose transport into the bacterium (Km for maltose, 0.8 microM, Ki for methyl-alpha-maltoside, 5.5 microM; Ki for 5-thiomaltose, 0.2 microM). Both analogs are bound by the periplasmic maltose-binding protein. Methyl-alpha-[14C]maltoside and 5-[3H]thiomaltose were both accumulated inside E. coli. Methyl-alpha-maltoside was unchanged after accumulation, but 5-thiomaltose was converted to an unidentified compound that could exit from the bacterium. Both analogs were inhibitory to the growth of E. coli, but only when the bacteria were previously induced for the maltose transport system. The analogs are substrates for but poor inducers of the maltose transport system.

Links

PubMed

Keywords

Biological Transport; Carrier Proteins; Escherichia coli; Maltose; Membrane Proteins

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain:
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: malE
  • Genotype of Experimental Strain : malE(del)
  • Reference Condition:

OMP:0000284

loss of utilization of carbon-containing compound

ECO:0000033

traceable author statement

Thio(5)maltose increase resistance for selection for mutant maltose transport system.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain:
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: malF
  • Genotype of Experimental Strain : malF(del)
  • Reference Condition:

OMP:0000284

loss of utilization of carbon-containing compound

ECO:0000033

traceable author statement

Thio(5)maltose increase resistance for selection for mutant maltose transport system.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain:
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: malG
  • Genotype of Experimental Strain : malG(del)
  • Reference Condition:

OMP:0000284

loss of utilization of carbon-containing compound

ECO:0000033

traceable author statement

Thio(5)maltose increase resistance for selection for mutant maltose transport system.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain:
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: malK
  • Genotype of Experimental Strain : malK(del)
  • Reference Condition:

OMP:0000284

loss of utilization of carbon-containing compound

ECO:0000033

traceable author statement

Thio(5)maltose resistance.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain:
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: malT
  • Genotype of Experimental Strain : malT(del)
  • Reference Condition:

OMP:0000284

loss of utilization of carbon-containing compound

ECO:0000033

traceable author statement

Thio(5)maltose resistance.


Notes

References

See Help:References for how to manage references in OMPwiki.