PMID:6757665

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Citation

Dabbs, ER (1982) Three additional loci of rifampicin dependence in Escherichia coli.Mol. Gen. Genet. 187:519-22

Abstract

Rifampicin dependent mutants had been isolated as conditional lethal mutants with a lesion affecting transcription. Substituting Tn10 coded tetracycline resistance as a stable marker instead of the unstable rifampicin dependence facilitated mapping of dependence mutations. Three new rid loci were defined in this way. ridB and ridD were on opposite sides of ilv at about 84 min, and ridC was at about 35 min on the Escherichia coli chromosomal linkage map. Two more mutants with the previously described ridA lesion were also found. Growth response to removal of rifampicin was different for ridA, B, or C strains. Double mutants were constructed, and in all cases the more limiting phenotypic response prevailed.

Links

PubMed

Keywords

Chromosome Mapping; Chromosomes, Bacterial; Crosses, Genetic; Escherichia coli; Genes, Lethal; Genotype; Mutation; Phenotype; Rifampin; Transcription, Genetic

Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: PB61
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: rpoB ridB
  • Genotype of Experimental Strain : rpoB361 ridB1
  • Reference Condition: Rich medium and between 30 and 37C
OMP:0000276

increased resistance to antimicrobial compound

ECO:0000182

in vitro culture assay data

Mutant is dependent on rifampicin. with the condition its grown below 42C in rich medium. Table 3 and figure 1


Notes

Needs OMP term. Dependence on antibiotic. A term request was submitted by Will, waiting on that term.

References

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