PMID:6266910

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Citation

Defez, R and De Felice, M (1981) Cryptic operon for beta-glucoside metabolism in Escherichia coli K12: genetic evidence for a regulatory protein.Genetics 97:11-25

Abstract

Escherichia coli K12 does not metabolize beta-glucosides such as arbutin and salicin because of lack of expression of the bglBSRC operon, which contains structural genes for transport (bglC) and hydrolysis (bglB) of phospho-beta-glucosides. Mutants carrying lesions in the cis-acting regulatory site bglR metabolize beta-glucosides as a consequence of expression of this cryptic operon (Prasad and Schaefler 1974). We isolated mutations promoting beta-glucoside metabolism that were unlinked to bglR; some of these mutations were shown to be amber. All of them were mapped at 27 min on the E. coli K12 linkage map and appeared to define a single gene, for which we propose the designation bglY. Utilization of beta-glucosides in bglY mutants appeared to be a consequence of expression of the bglBSRC operon, since bglB bglR and bglB bglY double mutants had the same phenotype. All bglY mutations analyzed were recessive to the wild-type bglY+ allele. Phospho-beta-glucosidase B and beta-glucoside transport activities are inducible in bglY mutants, as they are in bglR mutants. Metabolism of beta-glucosides in both bglR and bglY mutants required cyclic AMP. We propose that bglY encodes a protein acting as a repressor of the bglBSRC operon, active in both the presence and absence of beta-glucosides, whose recognition site would be within the bglR locus.

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PubMed

Keywords

Carrier Proteins; Chromosome Mapping; Chromosomes, Bacterial; Cyclic AMP; Escherichia coli; Gene Expression Regulation; Genes, Regulator; Glucosides; Glycosides; Mutation; Phenotype; beta-Glucosidase

Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain:
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: bglY
  • Genotype of Experimental Strain : bglY202
  • Reference Condition:

OMP:0000266

utilizes carbon-containing compound

ECO:0000182

in vitro culture assay data

Utilization of arbutin (a Beta-glucoside) as a carbon source, table 3.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain:
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: blgY
  • Genotype of Experimental Strain : bglY203
  • Reference Condition:

OMP:0000266

utilizes carbon-containing

ECO:0000182

in vitro culture assay data

able to utilize arbutin and salicin as a carbon source, table 3. Mutations also utilizing similar carbon sources are: bglY204, bglY205, bglY206, bglY207


Notes

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