PMID:6257656

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Citation

Fouts, KE and Barbour, SD (1981) Transductional mapping of ksgB and a new Tn5-induced kasugamycin resistance gene, ksgD, in Escherichia coli K-12.J. Bacteriol. 145:914-9

Abstract

We have mapped the Escherichia coli ksgB gene to min 36.5, 0.8 min from man and 0.7 min from aroD. A new kasugamycin resistance (Ksgr) gene, ksgD, has been isolated, using a transposon, Tn5. ksgD::TN5 is 44% cotransducible with sbcA, unlinked to trp, and unlinked to man (by P1 transduction). The ksgD::Tn5 has a late time of entry from HfrB7 (PO43). These data place ksgD clockwise from sbcA (which enters early from HfrB7) at min 30.4. The reistance of ksgB ksgD single and double mutant strains has been quantitated. Single mutations, ksgB or ksgD, gave resistance to 600 micrograms of kasugamycin per ml, whereas a ksgB ksgD strain was able to grow in the presence of kasugamycin levels in excess of 3,000 micrograms/ml. This indicates that the mechanisms of resistance coded for by the two genes are independent and synergistic.

Links

PubMed

Keywords

Aminoglycosides; Anti-Bacterial Agents; Chromosome Mapping; Chromosomes, Bacterial; Conjugation, Genetic; DNA Transposable Elements; DNA, Bacterial; Drug Resistance, Microbial; Escherichia coli; Genes; Transduction, Genetic

Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JC5029
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: ksgD
  • Genotype of Experimental Strain : ksgD101:Tn5
  • Reference Condition:
OMP:0000276

increased resistance to antimicrobial compound

Increase resistane to Aminoglycoside Kasugamycin Antibiotic at consentrations up to 600 µg/ml. In strain: SDB1171

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JC5029
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: ksgB
  • Genotype of Experimental Strain : ksgB1
  • Reference Condition:
OMP:0000276

increased resistance to antimicrobial compound

Increase resistane to Aminoglycoside Kasugamycin Antibiotic at consentrations up to 600 µg/ml. In strain: SDB1170

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JC5029
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: ksgD ksgB
  • Genotype of Experimental Strain : ksgB1 ksgD101::Tn5
  • Reference Condition:
OMP:0000276

increased resistance to antimicrobial compound

Increase resistane to Aminoglycoside Kasugamycin Antibiotic at consentrations up to 600 µg/ml


Notes

ECOID: Solid plate assay.

OMPterm: It is an aminoglycoside and the term is waiting to be made.

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