PMID:4570603

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Citation

Sparling, PF, Ikeya, Y and Elliot, D (1973) Two genetic loci for resistance to kasugamycin in Escherichia coli.J. Bacteriol. 113:704-10

Abstract

There are two loci for resistance to the antibiotic kasugamycin (Ksg) in Escherichia coli. Mutations at ksgA resulted in 30S ribosomal subunit resistance to Ksg. The map location of ksgA was near minute 0.5: ksgA was 95% cotransducible with pdxA, and the apparent gene order was thr... ksgA... pdxA. Studies in stable ksgA/ksgA(+) merodiploids showed that sensitivity was dominant over resistance. Mutations at a second gene (ksgB), located between minutes 25 and 39, resulted in phenotypic Ksg(R) indistinguishable from ksgA mutations, but ribosomes from ksgB strains were sensitive to the drug in vitro. Spontaneous and induced mutations to Ksg(R) were usually of the ksgA (ribosomal) type.

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Keywords

Alleles; Anti-Bacterial Agents; Carbon Isotopes; Cell-Free System; Chromosome Mapping; Chromosomes, Bacterial; Conjugation, Genetic; Drug Resistance, Microbial; Escherichia coli; Genes; Glycosides; Mutagens; Mutation; Nitrosoguanidines; Recombination, Genetic; Ribosomes; Transduction, Genetic; Valine

Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JC12
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: ksgA ksgB
  • Genotype of Experimental Strain : ksgA23 ksgB1
  • Reference Condition:
OMP:0000276

increased resistance to antimicrobial compound

Double mutant is Resistant to Kasugamycin at levels greater than 2,000 µg/ml. See table 5.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JC411
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: ksgA
  • Genotype of Experimental Strain : ksgA19
  • Reference Condition:
OMP:0000276

increased resistance to antimicrobial compound

Resistant to Aminoglycoside Kasugamycin at concentrations at 1,000 µg/ml. Mutant strain: FS232, on table 5.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JC411
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: ksgA
  • Genotype of Experimental Strain : ksgA19
  • Reference Condition:
OMP:0000276

increased resistance to antimicrobial compound

Resistant to Aminoglycoside Kasugamycin at concentrations at 2,000 µg/ml. Mutant strain: FS131, on table 5.


Notes

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