PMID:4555418

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Citation

Venkateswaran, PS and Wu, HC (1972) Isolation and characterization of a phosphonomycin-resistant mutant of Escherichia coli K-12.J. Bacteriol. 110:935-44

Abstract

A mutant was isolated from Escherichia coli K-12 which showed increased resistance towards phosphonomycin, a new bactericidal antibiotic recently isolated from strains of Streptomyces. Evidence is presented which suggests that this mutant is resistant to lysis by phosphonomycin because of a lower affinity of phosphoenolpyruvate: uridine diphospho-N-acetylglucosamine enolpyruvyl transferase for this antibiotic. This mutant was also found to be temperature-sensitive in growth. At 42 C mutant cells grew poorly, and the rate of incorporation of (3)H-diaminopimelic acid into trichloroacetic acid-insoluble material was also greatly reduced. Genetic studies indicate that the increased resistance toward phosphonomycin and temperature sensitivity in growth of this mutant are probably the consequences of a single mutation.

Links

PubMed

Keywords

Anti-Bacterial Agents; Carbon Isotopes; Cell Wall; Chromatography, Paper; Culture Media; Drug Resistance, Microbial; Escherichia coli; Genetics, Microbial; Glucose; Glucosephosphates; Glycerophosphates; Microscopy, Phase-Contrast; Mutation; Phosphoenolpyruvate; Phosphotransferases; Pimelic Acids; Temperature; Tritium

Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain:
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: glpT
  • Genotype of Experimental Strain : glpT -
  • Reference Condition:
OMP:0000276

increased resistance to antimicrobial compound

Phosphonomycin resistant

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain:
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: uhp
  • Genotype of Experimental Strain : uhp -
  • Reference Condition:
OMP:0000276

increased resistance to antimicrobial compound

Phosphonomycin resistant

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain:
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: ptsI
  • Genotype of Experimental Strain : ptsI
  • Reference Condition:
  • Experimental Condition: Slow growth at 42C
OMP:0000276

increased resistance to antimicrobial compound

Phosphonomycin resistant

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain:
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: ptsH
  • Genotype of Experimental Strain : ptsH
  • Reference Condition: Slow growth at 42C
OMP:0000276

increased resistance to antimicrobial compound

Phosphonomycin resistant


Notes

Salmonella gene[1]


needs broth culture OMP term.

References

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  1. Cordaro, JC et al. (1976) Fosfomycin resistance: selection method for internal and extended deletions of the phosphoenolpyruvate:sugar phosphotransferase genes of Salmonella typhimurium. J. Bacteriol. 128 785-93 PubMed OMPwiki page