PMID:21541325

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Citation

Deininger, KN, Horikawa, A, Kitko, RD, Tatsumi, R, Rosner, JL, Wachi, M and Slonczewski, JL (2011) A requirement of TolC and MDR efflux pumps for acid adaptation and GadAB induction in Escherichia coli.PLoS ONE 6:e18960

Abstract

The TolC outer membrane channel is a key component of several multidrug resistance (MDR) efflux pumps driven by H(+) transport in Escherichia coli. While tolC expression is under the regulation of the EvgA-Gad acid resistance regulon, the role of TolC in growth at low pH and extreme-acid survival is unknown.

Links

PubMed Online version:10.1371/journal.pone.0018960

Keywords

Acids; Adaptation, Physiological; Bacterial Outer Membrane Proteins; Drug Resistance, Multiple; Enzyme Induction; Escherichia coli K12; Escherichia coli Proteins; Glutamate Decarboxylase; Glutamates; Hydrogen-Ion Concentration; Membrane Proteins; Membrane Transport Proteins; Microbial Viability; Mutation

Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JLS1024
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: mdtB+
  • Genotype of Experimental Strain : mdtB774(del)::kan
  • Reference Condition:
OMP:0005077

decreased resistance to acidic pH stress

Figure 1: decreased survival at pH 2.

JLS1024 = W3110 mdtB774(del)::kan (Keio JW2060)

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JLS1015
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: tolC+
  • Genotype of Experimental Strain : tolC732(del)::kan
  • Reference Condition:
OMP:0005077

decreased resistance to acidic pH stress

Figure 1: decreased survival at pH 2.

JLS1015 = W3110 tolC732(del)::kan (Keio JW5503)

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JLS1027
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: emrB+
  • Genotype of Experimental Strain : emrB767(del)::kan
  • Reference Condition:
OMP:0005077

decreased resistance to acidic pH stress

Figure 1: decreased survival at pH 2.

JLS1027 = W3110 emrB767(del)::kan (Keio JW2661)

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JLS1015
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: tolC+
  • Genotype of Experimental Strain : tolC732(del)::kan
  • Reference Condition:
OMP:0007008

decreased growth at acidic pH

Figure 4: no growth at pH 4.5

JLS1015 = W3110 tolC732(del)::kan (Keio JW5503)

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: MG1655
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: tolC+
  • Genotype of Experimental Strain : tolC::Tn10
  • Reference Condition:
OMP:0007118

decreased transcription

ECO:0000106

Northern assay evidence

Figure 2B: undetectable level of gadA mRNA at pH 5.5

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: MG1655
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: tolC+
  • Genotype of Experimental Strain : tolC::Tn10
  • Reference Condition:
OMP:0000268

decreased gene expression

Figures 2A, 2C: Decreased glutamate decarboxylase activity and decreased GadA/B protein levels at pH 5.5.



Notes

References

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