PMID:21453489

From OMPwiki
Jump to: navigation, search
Citation

Poteete, AR (2011) Recombination phenotypes of Escherichia coli greA mutants.BMC Mol. Biol. 12:12

Abstract

The elongation factor GreA binds to RNA polymerase and modulates transcriptional pausing. Some recent research suggests that the primary role of GreA may not be to regulate gene expression, but rather, to promote the progression of replication forks which collide with RNA polymerase, and which might otherwise collapse. Replication fork collapse is known to generate dsDNA breaks, which can be recombinogenic. It follows that GreA malfunction could have consequences affecting homologous recombination.

Links

PubMed Online version:10.1186/1471-2199-12-12

Keywords

Escherichia coli; Escherichia coli Proteins; Mutation; Phenotype; Rec A Recombinases; Recombination, Genetic; Transcription Factors

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: MG1655
  • NCBI Taxon ID: 8333
  • Genotype of Reference Strain: greA+
  • Genotype of Experimental Strain : greA-D41A
  • Reference Condition:
OMP:0005050

decreased frequency of homologous recombination

Figure 1 and Figure 2: decreased lambda Red-mediated recombination

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: MG1655
  • NCBI Taxon ID: 8333
  • Genotype of Reference Strain: greA+
  • Genotype of Experimental Strain : greA-D41N
  • Reference Condition:
OMP:0005050

decreased frequency of homologous recombination

Figure 1 and Figure 2: decreased lambda Red-mediated recombination

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: MG1655
  • NCBI Taxon ID: 8333
  • Genotype of Reference Strain: greA+
  • Genotype of Experimental Strain : greA-E44K
  • Reference Condition:
OMP:0005050

decreased frequency of homologous recombination

Figure 1 and Figure 2: decreased lambda Red-mediated recombination

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: MG1655
  • NCBI Taxon ID: 8333
  • Genotype of Reference Strain: greA+
  • Genotype of Experimental Strain : greA(del)
  • Reference Condition:
OMP:0005050

decreased frequency of homologous recombination

Figure 1 and Figure 2: decreased lambda Red-mediated recombination



Notes

References

See Help:References for how to manage references in OMPwiki.