PMID:201607

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Citation

Iwaya, M, Jones, CW, Khorana, J and Strominger, JL (1978) Mapping of the mecillinam-resistant, round morphological mutants of Escherichia coli.J. Bacteriol. 133:196-202

Abstract

Genes responsible for round morphology in mecillinam-resistant, round morphological mutants of Escherichia coli have been mapped. Three mutants, called rodX, mapped at around 14 min, and two, called rodY, mapped at around 70 min by P1 transduction. These are either the same or very close to the loci reported, respectively, for the rodA (H. Matsuzawa, K. Hayakawa, T. Sato, and K. Imahori, J. Bacteriol. 115:436-442, 1973) and envB genes (B. Westling-Häggström and S. Normark, J. Bacteriol. 123:75-82, 1975). This suggests that mecillinam can be used very efficiently to select for found morphological mutants of rodA and envB after nitrosoguanidine treatment.

Links

PubMed

Keywords

Amdinocillin; Chromosome Mapping; Escherichia coli; Genes; Mutation; Penicillanic Acid; Penicillin Resistance; Phenotype; Transduction, Genetic

Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Eshcerichia coli
  • Strain: K-12
  • Substrain: HL18
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: rodY
  • Genotype of Experimental Strain : rodY -
  • Reference Condition:
OMP:0000276

increased resistance to antibiotics

Resistance to mecillinam

a mutation or genetic difference within a strain

  • Taxon: Eshcerichia coli
  • Strain: K-12
  • Substrain: HL18
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: rodY
  • Genotype of Experimental Strain : rodY -
  • Reference Condition:
OMP:0000075

coccus

Round morphology

a mutation or genetic difference within a strain

  • Taxon: Eshcerichia coli
  • Strain: K-12
  • Substrain: H3, HL29, HL103
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: rodX
  • Genotype of Experimental Strain : rodX -
  • Reference Condition:
OMP:0000075

coccus

Round morphology

a mutation or genetic difference within a strain

  • Taxon: Eshcerichia coli
  • Strain: K-12
  • Substrain: H3, HL29, HL103
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: rodX
  • Genotype of Experimental Strain : rodX -
  • Reference Condition:
OMP:0000276

increased resistance to antibiotics

Resistance to mecillinam


Notes

OMP term requested (increase resistance to Mecillinam a beta lactam). Also Solid Medium term has been requested (In vivo Assay in solid medium)

References

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