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Ammer, J, Brennenstuhl, M, Schindler, P, Höltje, JV and Zähner, H (1979) Phosphorylation of streptozotocin during uptake via the phosphoenolpyruvate: sugar phosphotransferase system in Escherichia coli.Antimicrob. Agents Chemother. 16:801-7


Mutants of Escherichia coli K-12, Staphylococcus aureus, and Bacillus subtilis defective in the general components (enzyme I, or HPr, or both) of the phosphoenolpyruvate:sugar phosphotransferase system are shown to be resistant to the antibiotic streptozotocin. It is shown here, employing 32P-labeled phosphoenolpyruvate, that wild-type cells of E. coli phosphorylate streptozotocin, whereas with a phosphotransferase system-defective mutant of E. coli the drug is recovered in an unaltered, free form. The internal accumulation of streptozotocin at the steady-state level was about 70 times that of the concentration in the external medium. The antibacterial action of streptozotocin, as well as the uptake of the drug, was inhibited by N-acetyl-D-glucosamine. The uptake of the antibiotic was extremely sensitive to p-chloromercuribenzoate. It is concluded that streptozotocin is taken up by E. coli via the phosphoenolpyruvate:sugar phosphotransferase system and consequently accumulates in the cell at first as streptozotocin-phosphate.




Acid Phosphatase; Drug Resistance, Microbial; Escherichia coli; Microbial Sensitivity Tests; Mutation; Phosphoenolpyruvate Sugar Phosphotransferase System; Phosphorylation; Streptozocin

Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: L191
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: ptsHI
  • Genotype of Experimental Strain : ptsHI
  • Reference Condition:

increased resistance to antibiotics


in vivo assay data

Double mutant is resistant to streptozotocin



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