PMID:10940054

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Citation

Zhao, J and Winkler, ME (2000) Reduction of GC --> TA transversion mutation by overexpression of MutS in Escherichia coli K-12.J. Bacteriol. 182:5025-8

Abstract

Overexpression of the MutS repair protein significantly decreased the rate of lacZ GC --> TA transversion mutation in stationary-phase and exponentially growing bacteria and in mutY and mutM mutants, which accumulate mismatches between 8-oxoguanine (8-oxoG) and adenine residues in DNA. Conversely, GC --> TA transversion increased in mutL or mutS mutants in stationary phase. In contrast, overexpression of MutS did not appreciably reduce lacZ AT --> CG transversion mutation in a mutT mutant. These results suggest that MutS-dependent repair can correct 8-oxoG:A mismatches in Escherichia coli cells but may not be able to compete with mutation fixation by MutY in mutT mutants.

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Keywords

Adenine; Adenosine Triphosphatases; Bacterial Proteins; Base Pair Mismatch; Cystine; DNA Repair; DNA-Binding Proteins; Escherichia coli; Escherichia coli Proteins; Gene Expression; Genes, Bacterial; Guanine; MutS DNA Mismatch-Binding Protein; Mutation; Thymine

Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: MG1655
  • NCBI Taxon ID: 511145
  • Genotype of Reference Strain: mutS+
  • Genotype of Experimental Strain : mutS+ overexpression
  • Reference Condition:
OMP:0005069

decreased mutation frequency

Overexpression of MutS decreases the rate of GC to TA transversion mutations.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: MG1655
  • NCBI Taxon ID: 511145
  • Genotype of Reference Strain: mutY::mini-Tn10(Tetr) mutS+
  • Genotype of Experimental Strain : mutY::mini-Tn10(Tetr) mutS+ overexpression
  • Reference Condition:
OMP:0005069

decreased mutation frequency

Overexpression of MutS decreases the rate of GC to TA transversion mutations in a strain lacking MutY.


Notes

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