PMID:10893421

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Citation

Davis, MS , Solbiati, J and Cronan, JE Jr (2000) Overproduction of acetyl-CoA carboxylase activity increases the rate of fatty acid biosynthesis in Escherichia coli. J. Biol. Chem. 275:28593-8

Abstract

Acetyl-CoA carboxylase (ACC) catalyzes the first committed step of the fatty acid synthetic pathway. Although ACC has often been proposed to be a major rate-controlling enzyme of this pathway, no direct tests of this proposal in vivo have been reported. We have tested this proposal in Escherichia coli. The genes encoding the four subunits of E. coli ACC were cloned in a single plasmid under the control of a bacteriophage T7 promoter. Upon induction of gene expression, the four ACC subunits were overproduced in equimolar amounts. Overproduction of the proteins resulted in greatly increased ACC activity with a concomitant increase in the intracellular level of malonyl-CoA. The effects of ACC overexpression on the rate of fatty acid synthesis were examined in the presence of a thioesterase, which provided a metabolic sink for fatty acid overproduction. Under these conditions ACC overproduction resulted in a 6-fold increase in the rate of fatty acid synthesis.

Links

PubMed Online version:10.1074/jbc.M004756200

Keywords

Acetyl-CoA Carboxylase/physiology; Escherichia coli/metabolism; Fatty Acids/biosynthesis

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Genotype of Reference Strain: accA
  • Genotype of Experimental Strain : accA ++
  • Reference Condition:
OMP:0000267

increased gene expression

ECO:0000178

in vivo assay evidence

Increase Acetyle COA Carboxylase activity upon over expression. See table 1.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Genotype of Reference Strain: accB
  • Genotype of Experimental Strain : accB ++
  • Reference Condition:
OMP:0000267

increased gene expression

ECO:0000178

in vivo assay evidence

Increase Acetyle COA Carboxylase activity upon over expression. See table 1.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Genotype of Reference Strain: accC
  • Genotype of Experimental Strain : accC ++
  • Reference Condition:
OMP:0000267

increased gene expression

ECO:0000178

in vivo assay evidence

Increase Acetyle COA Carboxylase activity upon over expression. See table 1.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Genotype of Reference Strain: accD
  • Genotype of Experimental Strain : accD ++
  • Reference Condition:
OMP:0000267

increased gene expression

ECO:0000178

in vivo assay evidence

Increase Acetyle COA Carboxylase activity upon over expression. See table 1.


Notes

References

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