Category:ECO:0001820 ! rapid amplification of cDNA ends polymerase chain reaction evidence

name: rapid amplification of cDNA ends polymerase chain reaction evidence
def: "A type of nucleotide sequencing assay evidence in which RT-PCR (cDNA synthesis) is first used to produce a cDNA copy of a region of the RNA transcript being investigated followed by PCR to capture either the unknown 5' or 3' end of the transcript for sequencing, depending on whether 5' RACE-PCR or 3' RACE-PCR are being undertaken." [ECO:SW, PMID:17498297, PMID:7685466]
synonym: "3’ RACE-PCR" EXACT []
synonym: "5’ RACE-PCR" EXACT []
synonym: "RACE PCR" EXACT [PMI:17498297]
is_a: ECO:0000219 ! nucleotide sequencing assay evidence
comment: RACE PCR is frequently used to verify transcription start sites relevant to the function of transcription factor binding sites such as repression.