PMID:4945191

2013-09-09T20:50:02Z

Kayla.c.lesage: Table edited by Kayla.c.lesage via TableEdit

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!align=left |Citation
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'''Eriksson-Grennberg, KR, Nordström, K and Englund, P''' (1971) Resistance of Escherichia coli to penicillins. IX. Genetics and physiology of class II ampicillin-resistant mutants that are galactose negative or sensitive to bacteriophage C21, or both.''J. Bacteriol.'' '''108''':1210-23
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!align=left |Abstract
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Ampicillin-resistant mutants of class II are determined by a doubling of chromosomally and episomally mediated ampicillin resistance on agar plates. Several mutants were isolated from a female as well as from an Hfr strain. The mutants differed from each other in various properties such as response to colicin E2 and sodium cholate, response to the phages T4 and C21, and fermentation of galactose. By conjugation and transduction experiments, it was shown that mutations in at least four loci gave the class II phenotype. The mutations were found to be in the galU gene, the ctr gene, and two new genes close to mtl denoted lpsA and lpsB. The carbohydrate compositions of the lipopolysaccharides of the mutants were investigated and found to be changed compared to the parent strains. GalU mutants lacked rhamnose and galactose and had 11% glucose compared to the parent strain. The lpsA mutant also lacked rhamnose and had only traces of galactose and 58% glucose, whereas the lpsB mutant contained 14% rhamnose, traces of galactose, and 81% glucose compared to the parent strain.
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!align=left |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4945191 PubMed]

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!align=left |Keywords
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Ampicillin; Carbon Isotopes; Cell Wall; Chromosome Mapping; Conjugation, Genetic; Crosses, Genetic; Escherichia coli; Fermentation; Galactose; Glucose; Isomerases; Lipopolysaccharides; Lysogeny; Mutation; Penicillin Resistance; Phenotype; Phosphoglucomutase; Rhamnose; Salmonella Phages; Transduction, Genetic

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==Main Points of the Paper ==
{{LitSignificance}}

== Materials and Methods Used ==
{{LitMaterials}}

==Phenotype Annotations==
{{AnnotationTableHelp}}
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="J4f9f068dce746" class=" tableEdit Phenotype_Table_2"
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
*Strain: K-12
*Substrain: D21
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''galU+''
*Genotype of Experimental Strain : ''galU'' in mutant strain D21e8
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*Reference Condition:
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Unable to utilize carbon source galactose, table 8
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
*Strain: K-12
*Substrain: D21e18
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''ptsI''
*Genotype of Experimental Strain : ''ptsI''
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*Reference Condition:
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resistant to phage C21
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
*Strain: K-12
*Substrain: D21e18
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''ptsI''
*Genotype of Experimental Strain : ''ptsI''
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*Reference Condition:
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Was unable to grown in minimal medium with glucose as sole carbon source. Table 2
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
*Strain: K-12
*Substrain: D21
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''ptsI''
*Genotype of Experimental Strain : ''ptsI'' in mutant strain D21e18
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*Reference Condition:
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OMP:0000276
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increased resistance to antimicrobial compound
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Ampicillin resistance in LA plates containing ampicillin.
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
*Strain: K-12
*Substrain: D21
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''galU''
*Genotype of Experimental Strain : ''galU'' in mutant strain D21e8
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*Reference Condition:
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OMP:0000276
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increased resistance to antimicrobial compound
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Ampicillin resistance in LA plates containing ampicillin.
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
*Strain: K-12
*Substrain: D21
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''lpsA''
*Genotype of Experimental Strain : ''lpsA'' in mutant strain D21e7
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*Reference Condition:
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OMP:0000276
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increased resistance to antimicrobial compound
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Ampicillin resistance in LA plates containing ampicillin.
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
*Strain: K-12
*Substrain: D21
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''lpsB''
*Genotype of Experimental Strain : ''lpsB'' in mutant strain D21e19
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*Reference Condition:
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OMP:0000276
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increased resistance to antimicrobial compound
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Ampicillin resistance in LA plates containing ampicillin.
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
*Strain: K-12
*Substrain: D21
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''galU''
*Genotype of Experimental Strain : ''galU'' in mutant strain D21e8
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*Reference Condition:
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Sensitive to sodium cholate, table 2
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
*Strain: K-12
*Substrain: D21
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''galU''
*Genotype of Experimental Strain : ''galU'' in mutant strain D21e8
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*Reference Condition:
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Sensitive to phage T4, table 2
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
*Strain: K-12
*Substrain: D21
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''galU''
*Genotype of Experimental Strain : ''galU'' in mutant strain D21e8
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*Reference Condition:
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Sensitive to phage C21, table 2. D21e8 and D21e10 contain a deletion covering galU gene.
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
*Strain: K-12
*Substrain: D21
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''galU''
*Genotype of Experimental Strain : ''galU'' in mutant strain D21e8
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*Reference Condition:
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Sensitive to phage P1bt, table 2
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==Notes==

==References==
{{RefHelp}}
<references/>

[[Category:Publication]] [[Category:Papers referenced in the LaRossa chapter]] [[Category:LaRossa citations that need review]]

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PMID:4945191