OMPwiki - User contributions [en]

PMID:1100857

2013-02-12T22:50:48Z

66.249.73.168: Fill PMID: Page!

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{| id="F511ac7486e243" class=" tableEdit PMID_info_table"

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!align=left |Citation
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'''Aswad, D and Koshland, DE Jr''' (1975) Isolation, characterization and complementation of Salmonella typhimurium chemotaxis mutants. ''J. Mol. Biol.'' '''97''':225-35
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!align=left |Abstract
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No abstract in PubMed
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!align=left |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1100857 PubMed]

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!align=left |Keywords
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Chemotaxis; Genes, Dominant; Genes, Recessive; Genetic Complementation Test; Genotype; Mutation; Recombination, Genetic; Salmonella typhimurium/isolation & purification; Salmonella typhimurium/physiology; Species Specificity

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==Main Points of the Paper ==
{{LitSignificance}}

== Materials and Methods Used ==
{{LitMaterials}}

==Phenotype Annotations==
{{AnnotationTableHelp}}
<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="P511ac74870c58" class=" tableEdit Phenotype_Table_2"
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status

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==Notes==

==References==
{{RefHelp}}
<references/>

[[Category:Publication]]

PMID:20474229

2013-01-16T11:58:30Z

66.249.73.168: Fill PMID: Page!

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{| id="O50f695e62f403" class=" tableEdit PMID_info_table"

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'''Macconkey, A ''' (1905) Lactose-Fermenting Bacteria in Faeces. ''J Hyg (Lond)'' '''5''':333-79
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!align=left |Abstract
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No abstract in PubMed
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!align=left |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=20474229 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2236133 PMC2236133]

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!align=left |Keywords
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==Main Points of the Paper ==
{{LitSignificance}}

== Materials and Methods Used ==
{{LitMaterials}}

==Phenotype Annotations==
{{AnnotationTableHelp}}
<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="V50f695e63176f" class=" tableEdit Phenotype_Table_2"
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status

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</protect>

==Notes==

==References==
{{RefHelp}}
<references/>

[[Category:Publication]]

PMID:20474363

2013-01-16T11:47:51Z

66.249.73.168: Fill PMID: Page!

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{| id="L50f6936724a40" class=" tableEdit PMID_info_table"

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!align=left |Citation
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'''Macconkey, AT ''' (1908) Bile Salt Media and their advantages in some Bacteriological Examinations. ''J Hyg (Lond)'' '''8''':322-34
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!align=left |Abstract
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No abstract in PubMed
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!align=left |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=20474363 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2167122 PMC2167122]

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!align=left |Keywords
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|}


==Main Points of the Paper ==
{{LitSignificance}}

== Materials and Methods Used ==
{{LitMaterials}}

==Phenotype Annotations==
{{AnnotationTableHelp}}
<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="Y50f6936731d53" class=" tableEdit Phenotype_Table_2"
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status

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|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.3411.Y50f6936731d53&page=3411&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table] || || || || || || || || ||
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</protect>

==Notes==

==References==
{{RefHelp}}
<references/>

[[Category:Publication]]

PMID:20474229

2013-01-16T11:46:50Z

66.249.73.168: Fill PMID: Page!

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{| id="C50f6932a76487" class=" tableEdit PMID_info_table"

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!align=left |Citation
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'''Macconkey, A ''' (1905) Lactose-Fermenting Bacteria in Faeces. ''J Hyg (Lond)'' '''5''':333-79
|-
!align=left |Abstract
||
No abstract in PubMed
|-
!align=left |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=20474229 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2236133 PMC2236133]

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!align=left |Keywords
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|}


==Main Points of the Paper ==
{{LitSignificance}}

== Materials and Methods Used ==
{{LitMaterials}}

==Phenotype Annotations==
{{AnnotationTableHelp}}
<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="O50f6932ab0781" class=" tableEdit Phenotype_Table_2"
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.3413.O50f6932ab0781&page=3413&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table] || || || || || || || || ||
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</protect>

==Notes==

==References==
{{RefHelp}}
<references/>

[[Category:Publication]]

PMID:11929534

2012-12-31T09:48:04Z

66.249.73.168: Fill PMID: Page!

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{| id="H50e15f544cc5c" class=" tableEdit PMID_info_table"

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'''Sperandio, V , Torres, AG and Kaper, JB ''' (2002) Quorum sensing Escherichia coli regulators B and C (QseBC): a novel two-component regulatory system involved in the regulation of flagella and motility by quorum sensing in E. coli. ''Mol. Microbiol.'' '''43''':809-21
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!align=left |Abstract
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Quorum sensing is a cell-to-cell signalling mechanism in which bacteria secrete hormone-like compounds called autoinducers. When these auto-inducers reach a certain threshold concentration, they interact with bacterial transcriptional regulators, thereby regulating gene expression. Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 as well as E. coli K-12 produces the autoinducer-2 (AI-2), which is synthesized by the product of the luxS gene, and previous work from our laboratory has shown that genes encoding the EHEC type III secretion system were activated by quorum sensing. Recently, by hybridizing an E. coli K-12 gene array with cDNA synthesized from RNA extracted from EHEC strain 86-24 and its isogenic luxS mutant, we observed that other potential virulence-associated factors, such as genes encoding the expression and assembly of flagella, motility and chemotaxis, were also activated by quorum sensing. The array data also indicated that several genes encoding putative E. coli regulators were controlled by quorum sensing. In this report, we describe a two-component system regulated by quorum sensing that shares homology with Salmonella typhimurium PmrAB, which we have named quorum sensing E. coli regulator B and C (QseBC). The qseBC genes, previously identified only as open reading frames b3025 and b3026, are organized in an operon in the E. coli chromosome, with qseB encoding the response regulator and qseC the sensor kinase. We confirmed the regulation of qseBC by quorum sensing using qseB::lacZ transcriptional fusions and characterized the phenotypes of an isogenic qseC mutation in EHEC. This mutant expressed less flagellin and had reduced motility compared with the wild-type and complemented strains. Transcription of flhD, fliA, motA and fliC::lacZ fusions was decreased in the qseC mutant, suggesting that qseBC is a transcriptional regulator of flagella genes. A qseC mutant was also generated in E. coli K-12 strain MC1000 that showed the same phenotypes as the EHEC mutant, indicating that qseBC regulates flagella and motility by quorum sensing in both EHEC and K-12. QseBC activates transcription of flhDC, which is the master regulator for the flagella and motility genes and, in the absence of flhD, QseBC failed to activate the transcription of fliA. Motility of a luxS, but not of a qseC, mutant can be restored by providing AI-2 exogenously as preconditioned media, suggesting that the qseC mutant is unable to respond to AI-2. However, QseC has no effect on the expression of other quorum sensing-controlled genes such as those encoding for the type III secretion system. These data indicate that QseBC is one component of the quorum-sensing regulatory cascade in both EHEC and K-12 that is involved in the regulation of flagella and motility genes, but that additional regulators in this cascade remain to be characterized.
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!align=left |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11929534 PubMed]

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!align=left |Keywords
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DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Escherichia coli/physiology; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Flagella/physiology; Gene Expression Regulation, Bacterial; Molecular Sequence Data; Mutation; Promoter Regions, Genetic; Regulon; Trans-Activators/genetics; Trans-Activators/metabolism; Transcription, Genetic

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|}


==Main Points of the Paper ==
{{LitSignificance}}

== Materials and Methods Used ==
{{LitMaterials}}

==Phenotype Annotations==
{{AnnotationTableHelp}}
<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="M50e15f5454fec" class=" tableEdit Phenotype_Table_2"
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.3338.M50e15f5454fec&page=3338&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table] || || || || || || || || ||
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</protect>

==Notes==

==References==
{{RefHelp}}
<references/>

[[Category:Publication]]

PMID:10103193

2012-12-30T23:39:15Z

66.249.73.168: Fill PMID: Page!

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{| id="L50e0d0a399111" class=" tableEdit PMID_info_table"

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!align=left |Citation
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'''Bagel, S , Hüllen, V , Wiedemann, B and Heisig, P ''' (1999) Impact of gyrA and parC mutations on quinolone resistance, doubling time, and supercoiling degree of Escherichia coli. ''Antimicrob. Agents Chemother.'' '''43''':868-75
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!align=left |Abstract
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Isogenic mutants derived from quinolone-susceptible isolate WT by introducing gyrA (S83L, D87G) and parC (S80I, E84K) mutations associated with quinolone resistance were characterized with respect to quinolone resistance, growth rate, and degree of global supercoiling. The latter was determined by use of a pair of reporter plasmids carrying supercoiling-dependent promoters pgyrA and ptopA, respectively, transcriptionally fused to the reporter gene bla coding for TEM-1 beta-lactamase. The quotient (Qsc) of the beta-lactamase specific activity determined for a mutant carrying either plasmid was taken as a measure of the degree of global supercoiling. These Qsc data were comparable to results obtained from the separation of topoisomers of plasmid pBR322 on chloroquine-containing agarose gels and indicate a reduced degree of negative supercoiling in resistant mutants relative to the parent, WT. The S83L mutation in gyrA had the strongest influence on quinolone resistance while leaving other parameters nearly unaffected. The gyrA double mutation (S83L plus D87G) had an effect on quinolone resistance similar to that of a single mutation. Phenotypic expression of the parC mutation (S80I) was dependent on the presence of at least one gyrA mutation. Expression of high-level fluoroquinolone resistance (ciprofloxacin MIC, > 4 micrograms/ml) required a combination of the gyrA double mutation and one parC mutation (S80I or E84K). Such mutants showed considerable alterations of growth rate, global supercoiling, or both. Introduction of a parC mutation affected neither the doubling time nor the degree of supercoiling, while the presence of the gyrA D87G mutation was associated with a significant reduction in the degree of DNA supercoiling.
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!align=left |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10103193 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC89219 PMC89219]

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!align=left |Keywords
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4-Quinolones; Anti-Infective Agents/pharmacology; Cell Division; DNA Gyrase; DNA Topoisomerase IV; DNA Topoisomerases, Type I/genetics; DNA Topoisomerases, Type II/genetics; DNA Topoisomerases, Type II/physiology; DNA, Bacterial/chemistry; Drug Resistance, Microbial/genetics; Escherichia coli/cytology; Escherichia coli/drug effects; Escherichia coli/genetics; Genotype; Humans; Mutation; Nucleic Acid Conformation; Phenotype

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|}


==Main Points of the Paper ==
{{LitSignificance}}

== Materials and Methods Used ==
{{LitMaterials}}

==Phenotype Annotations==
{{AnnotationTableHelp}}
<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="G50e0d0a3c7da2" class=" tableEdit Phenotype_Table_2"
|-
!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.3422.G50e0d0a3c7da2&page=3422&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table] || || || || || || || || ||
|}
</protect>

==Notes==

==References==
{{RefHelp}}
<references/>

[[Category:Publication]]

PMID:16356850

2012-10-13T13:49:15Z

66.249.73.168: New PMID: Page!

PMID:16356850

2012-10-13T13:49:15Z

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{| id="E5079715b54a6f" class=" tableEdit PMID_info_table"

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!align=left |Citation
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'''Sudarsan, N , Cohen-Chalamish, S , Nakamura, S , Emilsson, GM and Breaker, RR ''' (2005) Thiamine pyrophosphate riboswitches are targets for the antimicrobial compound pyrithiamine. ''Chem. Biol.'' '''12''':1325-35
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!align=left |Abstract
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Thiamine metabolism genes are regulated in numerous bacteria by a riboswitch class that binds the coenzyme thiamine pyrophosphate (TPP). We demonstrate that the antimicrobial action of the thiamine analog pyrithiamine (PT) is mediated by interaction with TPP riboswitches in bacteria and fungi. For example, pyrithiamine pyrophosphate (PTPP) binds the TPP riboswitch controlling the tenA operon in Bacillus subtilis. Expression of a TPP riboswitch-regulated reporter gene is reduced in transgenic B. subtilis or Escherichia coli when grown in the presence of thiamine or PT, while mutant riboswitches in these organisms are unresponsive to these ligands. Bacteria selected for PT resistance bear specific mutations that disrupt ligand binding to TPP riboswitches and derepress certain TPP metabolic genes. Our findings demonstrate that riboswitches can serve as antimicrobial drug targets and expand our understanding of thiamine metabolism in bacteria.
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!align=left |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16356850 PubMed]
Online version:[http://dx.doi.org/10.1016/j.chembiol.2005.10.007 10.1016/j.chembiol.2005.10.007]
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!align=left |Keywords
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Anti-Infective Agents/pharmacology; Bacillus/genetics; Models, Biological; Molecular Structure; Nucleic Acid Conformation; Protein Structure, Tertiary; Pyrithiamine/pharmacology; RNA, Bacterial/drug effects; Thiamine/chemistry; Thiamine/metabolism; Thiamine/pharmacology; Thiamine Pyrophosphate/biosynthesis; Thiamine Pyrophosphate/genetics; Thiamine Pyrophosphate/metabolism

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|}


==Main Points of the Paper ==
{{LitSignificance}}

== Materials and Methods Used ==
{{LitMaterials}}

==Phenotype Annotations==
{{AnnotationTableHelp}}
<protect>

{| id="Y5079715b57b54" class=" tableEdit Phenotype_Table_2"
|-
!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.3543.Y5079715b57b54&page=3543&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table] || || || || || || || || ||
|}
</protect>

==Notes==

==References==
{{RefHelp}}
<references/>

[[Category:Publication]]