OMPwiki - User contributions [en]

PMID:16738554

2016-03-14T03:42:21Z

66.249.69.233: Fill PMID: Page!

{{RightTOC}}



{| id="K56e6331cc86a7" class=" tableEdit PMID_info_table"

|-
!align=left align='left' bgcolor='#CCCCFF' |Citation
||
'''Baba, T, Ara, T, Hasegawa, M, Takai, Y, Okumura, Y, Baba, M, Datsenko, KA, Tomita, M, Wanner, BL and Mori, H''' (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. ''Mol. Syst. Biol.'' '''2''':2006.0008
|-
!align=left align='left' bgcolor='#CCCCFF' |Abstract
||
We have systematically made a set of precisely defined, single-gene deletions of all nonessential genes in Escherichia coli K-12. Open-reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by using a one-step method for inactivation of chromosomal genes and primers designed to create in-frame deletions upon excision of the resistance cassette. Of 4288 genes targeted, mutants were obtained for 3985. To alleviate problems encountered in high-throughput studies, two independent mutants were saved for every deleted gene. These mutants-the 'Keio collection'-provide a new resource not only for systematic analyses of unknown gene functions and gene regulatory networks but also for genome-wide testing of mutational effects in a common strain background, E. coli K-12 BW25113. We were unable to disrupt 303 genes, including 37 of unknown function, which are candidates for essential genes. Distribution is being handled via GenoBase (http://ecoli.aist-nara.ac.jp/).
|-
!align=left align='left' bgcolor='#CCCCFF' |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16738554 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1681482 PMC1681482]
Online version:[http://dx.doi.org/10.1038/msb4100050 10.1038/msb4100050]
|-
!align=left align='left' bgcolor='#CCCCFF' |Keywords
||
Escherichia coli/genetics; Gene Deletion; Internet; Mutation; Organisms, Genetically Modified

|- class="tableEdit_footer"
|<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.3345.K56e6331cc86a7&page=3345&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</div> ||
|}
<noinclude>

</noinclude>


==Main Points of the Paper ==
{{LitSignificance}}

== Materials and Methods Used ==
{{LitMaterials}}

==Phenotype Annotations==
{{AnnotationTableHelp}}
<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="K56e6331cd8b64" class=" tableEdit Phenotype_Table_2"
|- align='left' bgcolor='#CCCCFF'
!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status

|- class="tableEdit_footer"
|<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.3345.K56e6331cd8b64&page=3345&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table]</div> || || || || || || || || ||
|}
<noinclude>

</noinclude>
</protect>

==Notes==

==References==
{{RefHelp}}
<references/>

[[Category:Publication]]

PMID:16738554

2016-03-13T19:26:20Z

66.249.69.233: Fill PMID: Page!

{{RightTOC}}



{| id="I56e5bedc8767a" class=" tableEdit PMID_info_table"

|-
!align=left align='left' bgcolor='#CCCCFF' |Citation
||
'''Baba, T, Ara, T, Hasegawa, M, Takai, Y, Okumura, Y, Baba, M, Datsenko, KA, Tomita, M, Wanner, BL and Mori, H''' (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. ''Mol. Syst. Biol.'' '''2''':2006.0008
|-
!align=left align='left' bgcolor='#CCCCFF' |Abstract
||
We have systematically made a set of precisely defined, single-gene deletions of all nonessential genes in Escherichia coli K-12. Open-reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by using a one-step method for inactivation of chromosomal genes and primers designed to create in-frame deletions upon excision of the resistance cassette. Of 4288 genes targeted, mutants were obtained for 3985. To alleviate problems encountered in high-throughput studies, two independent mutants were saved for every deleted gene. These mutants-the 'Keio collection'-provide a new resource not only for systematic analyses of unknown gene functions and gene regulatory networks but also for genome-wide testing of mutational effects in a common strain background, E. coli K-12 BW25113. We were unable to disrupt 303 genes, including 37 of unknown function, which are candidates for essential genes. Distribution is being handled via GenoBase (http://ecoli.aist-nara.ac.jp/).
|-
!align=left align='left' bgcolor='#CCCCFF' |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16738554 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1681482 PMC1681482]
Online version:[http://dx.doi.org/10.1038/msb4100050 10.1038/msb4100050]
|-
!align=left align='left' bgcolor='#CCCCFF' |Keywords
||
Escherichia coli/genetics; Gene Deletion; Internet; Mutation; Organisms, Genetically Modified

|- class="tableEdit_footer"
|<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.3345.I56e5bedc8767a&page=3345&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</div> ||
|}
<noinclude>

</noinclude>


==Main Points of the Paper ==
{{LitSignificance}}

== Materials and Methods Used ==
{{LitMaterials}}

==Phenotype Annotations==
{{AnnotationTableHelp}}
<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="Q56e5bedc910cc" class=" tableEdit Phenotype_Table_2"
|- align='left' bgcolor='#CCCCFF'
!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status

|- class="tableEdit_footer"
|<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.3345.Q56e5bedc910cc&page=3345&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table]</div> || || || || || || || || ||
|}
<noinclude>

</noinclude>
</protect>

==Notes==

==References==
{{RefHelp}}
<references/>

[[Category:Publication]]

PMID:16738554

2016-03-13T19:26:18Z

66.249.69.233: Fill PMID: Page!

{{RightTOC}}



{| id="F56e5beda03bc9" class=" tableEdit PMID_info_table"

|-
!align=left align='left' bgcolor='#CCCCFF' |Citation
||
'''Baba, T, Ara, T, Hasegawa, M, Takai, Y, Okumura, Y, Baba, M, Datsenko, KA, Tomita, M, Wanner, BL and Mori, H''' (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. ''Mol. Syst. Biol.'' '''2''':2006.0008
|-
!align=left align='left' bgcolor='#CCCCFF' |Abstract
||
We have systematically made a set of precisely defined, single-gene deletions of all nonessential genes in Escherichia coli K-12. Open-reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by using a one-step method for inactivation of chromosomal genes and primers designed to create in-frame deletions upon excision of the resistance cassette. Of 4288 genes targeted, mutants were obtained for 3985. To alleviate problems encountered in high-throughput studies, two independent mutants were saved for every deleted gene. These mutants-the 'Keio collection'-provide a new resource not only for systematic analyses of unknown gene functions and gene regulatory networks but also for genome-wide testing of mutational effects in a common strain background, E. coli K-12 BW25113. We were unable to disrupt 303 genes, including 37 of unknown function, which are candidates for essential genes. Distribution is being handled via GenoBase (http://ecoli.aist-nara.ac.jp/).
|-
!align=left align='left' bgcolor='#CCCCFF' |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16738554 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1681482 PMC1681482]
Online version:[http://dx.doi.org/10.1038/msb4100050 10.1038/msb4100050]
|-
!align=left align='left' bgcolor='#CCCCFF' |Keywords
||
Escherichia coli/genetics; Gene Deletion; Internet; Mutation; Organisms, Genetically Modified

|- class="tableEdit_footer"
|<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.3345.F56e5beda03bc9&page=3345&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</div> ||
|}
<noinclude>

</noinclude>


==Main Points of the Paper ==
{{LitSignificance}}

== Materials and Methods Used ==
{{LitMaterials}}

==Phenotype Annotations==
{{AnnotationTableHelp}}
<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="B56e5beda0c979" class=" tableEdit Phenotype_Table_2"
|- align='left' bgcolor='#CCCCFF'
!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status

|- class="tableEdit_footer"
|<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.3345.B56e5beda0c979&page=3345&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table]</div> || || || || || || || || ||
|}
<noinclude>

</noinclude>
</protect>

==Notes==

==References==
{{RefHelp}}
<references/>

[[Category:Publication]]

PMID:16738554

2016-03-13T19:26:15Z

66.249.69.233: Fill PMID: Page!

{{RightTOC}}



{| id="A56e5bed74d183" class=" tableEdit PMID_info_table"

|-
!align=left align='left' bgcolor='#CCCCFF' |Citation
||
'''Baba, T, Ara, T, Hasegawa, M, Takai, Y, Okumura, Y, Baba, M, Datsenko, KA, Tomita, M, Wanner, BL and Mori, H''' (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. ''Mol. Syst. Biol.'' '''2''':2006.0008
|-
!align=left align='left' bgcolor='#CCCCFF' |Abstract
||
We have systematically made a set of precisely defined, single-gene deletions of all nonessential genes in Escherichia coli K-12. Open-reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by using a one-step method for inactivation of chromosomal genes and primers designed to create in-frame deletions upon excision of the resistance cassette. Of 4288 genes targeted, mutants were obtained for 3985. To alleviate problems encountered in high-throughput studies, two independent mutants were saved for every deleted gene. These mutants-the 'Keio collection'-provide a new resource not only for systematic analyses of unknown gene functions and gene regulatory networks but also for genome-wide testing of mutational effects in a common strain background, E. coli K-12 BW25113. We were unable to disrupt 303 genes, including 37 of unknown function, which are candidates for essential genes. Distribution is being handled via GenoBase (http://ecoli.aist-nara.ac.jp/).
|-
!align=left align='left' bgcolor='#CCCCFF' |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16738554 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1681482 PMC1681482]
Online version:[http://dx.doi.org/10.1038/msb4100050 10.1038/msb4100050]
|-
!align=left align='left' bgcolor='#CCCCFF' |Keywords
||
Escherichia coli/genetics; Gene Deletion; Internet; Mutation; Organisms, Genetically Modified

|- class="tableEdit_footer"
|<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.3345.A56e5bed74d183&page=3345&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</div> ||
|}
<noinclude>

</noinclude>


==Main Points of the Paper ==
{{LitSignificance}}

== Materials and Methods Used ==
{{LitMaterials}}

==Phenotype Annotations==
{{AnnotationTableHelp}}
<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="N56e5bed75282a" class=" tableEdit Phenotype_Table_2"
|- align='left' bgcolor='#CCCCFF'
!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status

|- class="tableEdit_footer"
|<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.3345.N56e5bed75282a&page=3345&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table]</div> || || || || || || || || ||
|}
<noinclude>

</noinclude>
</protect>

==Notes==

==References==
{{RefHelp}}
<references/>

[[Category:Publication]]

PMID:16738554

2016-01-30T00:09:05Z

66.249.69.233: Fill PMID: Page!

{{RightTOC}}



{| id="Q56abff218b982" class=" tableEdit PMID_info_table"

|-
!align=left align='left' bgcolor='#CCCCFF' |Citation
||
'''Baba, T, Ara, T, Hasegawa, M, Takai, Y, Okumura, Y, Baba, M, Datsenko, KA, Tomita, M, Wanner, BL and Mori, H''' (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. ''Mol. Syst. Biol.'' '''2''':2006.0008
|-
!align=left align='left' bgcolor='#CCCCFF' |Abstract
||
We have systematically made a set of precisely defined, single-gene deletions of all nonessential genes in Escherichia coli K-12. Open-reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by using a one-step method for inactivation of chromosomal genes and primers designed to create in-frame deletions upon excision of the resistance cassette. Of 4288 genes targeted, mutants were obtained for 3985. To alleviate problems encountered in high-throughput studies, two independent mutants were saved for every deleted gene. These mutants-the 'Keio collection'-provide a new resource not only for systematic analyses of unknown gene functions and gene regulatory networks but also for genome-wide testing of mutational effects in a common strain background, E. coli K-12 BW25113. We were unable to disrupt 303 genes, including 37 of unknown function, which are candidates for essential genes. Distribution is being handled via GenoBase (http://ecoli.aist-nara.ac.jp/).
|-
!align=left align='left' bgcolor='#CCCCFF' |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16738554 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1681482 PMC1681482]
Online version:[http://dx.doi.org/10.1038/msb4100050 10.1038/msb4100050]
|-
!align=left align='left' bgcolor='#CCCCFF' |Keywords
||
Escherichia coli/genetics; Gene Deletion; Internet; Mutation; Organisms, Genetically Modified

|- class="tableEdit_footer"
|<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.3345.Q56abff218b982&page=3345&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</div> ||
|}
<noinclude>

</noinclude>


==Main Points of the Paper ==
{{LitSignificance}}

== Materials and Methods Used ==
{{LitMaterials}}

==Phenotype Annotations==
{{AnnotationTableHelp}}
<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="N56abff2190deb" class=" tableEdit Phenotype_Table_2"
|- align='left' bgcolor='#CCCCFF'
!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status

|- class="tableEdit_footer"
|<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.3345.N56abff2190deb&page=3345&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table]</div> || || || || || || || || ||
|}
<noinclude>

</noinclude>
</protect>

==Notes==

==References==
{{RefHelp}}
<references/>

[[Category:Publication]]

PMID:16738554

2016-01-30T00:09:00Z

66.249.69.233: Fill PMID: Page!

{{RightTOC}}



{| id="W56abff1c264ab" class=" tableEdit PMID_info_table"

|-
!align=left align='left' bgcolor='#CCCCFF' |Citation
||
'''Baba, T, Ara, T, Hasegawa, M, Takai, Y, Okumura, Y, Baba, M, Datsenko, KA, Tomita, M, Wanner, BL and Mori, H''' (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. ''Mol. Syst. Biol.'' '''2''':2006.0008
|-
!align=left align='left' bgcolor='#CCCCFF' |Abstract
||
We have systematically made a set of precisely defined, single-gene deletions of all nonessential genes in Escherichia coli K-12. Open-reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by using a one-step method for inactivation of chromosomal genes and primers designed to create in-frame deletions upon excision of the resistance cassette. Of 4288 genes targeted, mutants were obtained for 3985. To alleviate problems encountered in high-throughput studies, two independent mutants were saved for every deleted gene. These mutants-the 'Keio collection'-provide a new resource not only for systematic analyses of unknown gene functions and gene regulatory networks but also for genome-wide testing of mutational effects in a common strain background, E. coli K-12 BW25113. We were unable to disrupt 303 genes, including 37 of unknown function, which are candidates for essential genes. Distribution is being handled via GenoBase (http://ecoli.aist-nara.ac.jp/).
|-
!align=left align='left' bgcolor='#CCCCFF' |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16738554 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1681482 PMC1681482]
Online version:[http://dx.doi.org/10.1038/msb4100050 10.1038/msb4100050]
|-
!align=left align='left' bgcolor='#CCCCFF' |Keywords
||
Escherichia coli/genetics; Gene Deletion; Internet; Mutation; Organisms, Genetically Modified

|- class="tableEdit_footer"
|<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.3345.W56abff1c264ab&page=3345&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</div> ||
|}
<noinclude>

</noinclude>


==Main Points of the Paper ==
{{LitSignificance}}

== Materials and Methods Used ==
{{LitMaterials}}

==Phenotype Annotations==
{{AnnotationTableHelp}}
<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="F56abff1c2f9f2" class=" tableEdit Phenotype_Table_2"
|- align='left' bgcolor='#CCCCFF'
!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status

|- class="tableEdit_footer"
|<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.3345.F56abff1c2f9f2&page=3345&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table]</div> || || || || || || || || ||
|}
<noinclude>

</noinclude>
</protect>

==Notes==

==References==
{{RefHelp}}
<references/>

[[Category:Publication]]

PMID:16738554

2016-01-30T00:08:57Z

66.249.69.233: Fill PMID: Page!

{{RightTOC}}



{| id="Z56abff190c8e4" class=" tableEdit PMID_info_table"

|-
!align=left align='left' bgcolor='#CCCCFF' |Citation
||
'''Baba, T, Ara, T, Hasegawa, M, Takai, Y, Okumura, Y, Baba, M, Datsenko, KA, Tomita, M, Wanner, BL and Mori, H''' (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. ''Mol. Syst. Biol.'' '''2''':2006.0008
|-
!align=left align='left' bgcolor='#CCCCFF' |Abstract
||
We have systematically made a set of precisely defined, single-gene deletions of all nonessential genes in Escherichia coli K-12. Open-reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by using a one-step method for inactivation of chromosomal genes and primers designed to create in-frame deletions upon excision of the resistance cassette. Of 4288 genes targeted, mutants were obtained for 3985. To alleviate problems encountered in high-throughput studies, two independent mutants were saved for every deleted gene. These mutants-the 'Keio collection'-provide a new resource not only for systematic analyses of unknown gene functions and gene regulatory networks but also for genome-wide testing of mutational effects in a common strain background, E. coli K-12 BW25113. We were unable to disrupt 303 genes, including 37 of unknown function, which are candidates for essential genes. Distribution is being handled via GenoBase (http://ecoli.aist-nara.ac.jp/).
|-
!align=left align='left' bgcolor='#CCCCFF' |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16738554 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1681482 PMC1681482]
Online version:[http://dx.doi.org/10.1038/msb4100050 10.1038/msb4100050]
|-
!align=left align='left' bgcolor='#CCCCFF' |Keywords
||
Escherichia coli/genetics; Gene Deletion; Internet; Mutation; Organisms, Genetically Modified

|- class="tableEdit_footer"
|<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.3345.Z56abff190c8e4&page=3345&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</div> ||
|}
<noinclude>

</noinclude>


==Main Points of the Paper ==
{{LitSignificance}}

== Materials and Methods Used ==
{{LitMaterials}}

==Phenotype Annotations==
{{AnnotationTableHelp}}
<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="F56abff1915640" class=" tableEdit Phenotype_Table_2"
|- align='left' bgcolor='#CCCCFF'
!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status

|- class="tableEdit_footer"
|<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.3345.F56abff1915640&page=3345&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table]</div> || || || || || || || || ||
|}
<noinclude>

</noinclude>
</protect>

==Notes==

==References==
{{RefHelp}}
<references/>

[[Category:Publication]]

PMID:16738554

2016-01-21T10:04:44Z

66.249.69.233: Fill PMID: Page!

{{RightTOC}}



{| id="N56a0ad3c8a3b3" class=" tableEdit PMID_info_table"

|-
!align=left align='left' bgcolor='#CCCCFF' |Citation
||
'''Baba, T, Ara, T, Hasegawa, M, Takai, Y, Okumura, Y, Baba, M, Datsenko, KA, Tomita, M, Wanner, BL and Mori, H''' (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. ''Mol. Syst. Biol.'' '''2''':2006.0008
|-
!align=left align='left' bgcolor='#CCCCFF' |Abstract
||
We have systematically made a set of precisely defined, single-gene deletions of all nonessential genes in Escherichia coli K-12. Open-reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by using a one-step method for inactivation of chromosomal genes and primers designed to create in-frame deletions upon excision of the resistance cassette. Of 4288 genes targeted, mutants were obtained for 3985. To alleviate problems encountered in high-throughput studies, two independent mutants were saved for every deleted gene. These mutants-the 'Keio collection'-provide a new resource not only for systematic analyses of unknown gene functions and gene regulatory networks but also for genome-wide testing of mutational effects in a common strain background, E. coli K-12 BW25113. We were unable to disrupt 303 genes, including 37 of unknown function, which are candidates for essential genes. Distribution is being handled via GenoBase (http://ecoli.aist-nara.ac.jp/).
|-
!align=left align='left' bgcolor='#CCCCFF' |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16738554 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1681482 PMC1681482]
Online version:[http://dx.doi.org/10.1038/msb4100050 10.1038/msb4100050]
|-
!align=left align='left' bgcolor='#CCCCFF' |Keywords
||
Escherichia coli/genetics; Gene Deletion; Internet; Mutation; Organisms, Genetically Modified

|- class="tableEdit_footer"
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==Main Points of the Paper ==
{{LitSignificance}}

== Materials and Methods Used ==
{{LitMaterials}}

==Phenotype Annotations==
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status

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==Notes==

==References==
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[[Category:Publication]]