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https://microbialphenotypes.org/wiki/index.php?title=PMID:16738554&diff=18077
PMID:16738554
2015-11-27T01:19:47Z
<p>151.80.31.119: Fill PMID: Page!</p>
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'''Baba, T, Ara, T, Hasegawa, M, Takai, Y, Okumura, Y, Baba, M, Datsenko, KA, Tomita, M, Wanner, BL and Mori, H''' (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. ''Mol. Syst. Biol.'' '''2''':2006.0008<br />
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!align=left align='left' bgcolor='#CCCCFF' |Abstract<br />
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We have systematically made a set of precisely defined, single-gene deletions of all nonessential genes in Escherichia coli K-12. Open-reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by using a one-step method for inactivation of chromosomal genes and primers designed to create in-frame deletions upon excision of the resistance cassette. Of 4288 genes targeted, mutants were obtained for 3985. To alleviate problems encountered in high-throughput studies, two independent mutants were saved for every deleted gene. These mutants-the 'Keio collection'-provide a new resource not only for systematic analyses of unknown gene functions and gene regulatory networks but also for genome-wide testing of mutational effects in a common strain background, E. coli K-12 BW25113. We were unable to disrupt 303 genes, including 37 of unknown function, which are candidates for essential genes. Distribution is being handled via GenoBase (http://ecoli.aist-nara.ac.jp/).<br />
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16738554 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1681482 PMC1681482]<br />
Online version:[http://dx.doi.org/10.1038/msb4100050 10.1038/msb4100050]<br />
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!align=left align='left' bgcolor='#CCCCFF' |Keywords<br />
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Escherichia coli/genetics; Gene Deletion; Internet; Mutation; Organisms, Genetically Modified<br />
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==Main Points of the Paper ==<br />
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== Materials and Methods Used ==<br />
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==Phenotype Annotations==<br />
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==Notes==<br />
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==References==<br />
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[[Category:Publication]]</div>
151.80.31.119