Difference between revisions of "OMP ST:928 ! Escherichia coli K-12 YK2008"

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!align=left align='left' bgcolor='#CCCCFF' |Strain Name
 
!align=left align='left' bgcolor='#CCCCFF' |Strain Name
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YK2008
 
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!align=left align='left' bgcolor='#CCCCFF' |Synonyms
 
!align=left align='left' bgcolor='#CCCCFF' |Synonyms
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!align=left align='left' bgcolor='#CCCCFF' |Taxon Information
 
!align=left align='left' bgcolor='#CCCCFF' |Taxon Information
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*Pangenome: Escherichia coli
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*Subspecies and/or strain: K-12
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*NCBI Taxonomy ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
 
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!align=left align='left' bgcolor='#CCCCFF' |Genotype
 
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F- lambda- e14-(?) Rac-0(?) uvrC34 hisG4(Oc) rfbC1 mgl-51 rpsL31(strR) kdgK51 xylA5 mtl-1 argE3(Oc) thiE1 thyA galU51 pyrC46 nalA flgE2008(Am)
 
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!align=left align='left' bgcolor='#CCCCFF' |Strain Reference
 
!align=left align='left' bgcolor='#CCCCFF' |Strain Reference
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PMID:338588
 
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!align=left align='left' bgcolor='#CCCCFF' |Strain availability
 
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!align=left align='left' bgcolor='#CCCCFF' |Ancestry
 
!align=left align='left' bgcolor='#CCCCFF' |Ancestry
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parent:OMP ST:927 ! Escherichia coli K-12 YK102
 
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!align=left align='left' bgcolor='#CCCCFF' |Annotated phenotypes
 
!align=left align='left' bgcolor='#CCCCFF' |Annotated phenotypes
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==Notes==
 
==Notes==
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YK2008 was isolated by selecting for derivatives of YK102 that are resistant to phage Chi and then screening for loss of motility. Note that in the paper<ref name='PMID:338588'/>, the flgE gene is referred to by the older name, flaK.
  
 +
For the flgE2008(Am) mutation, the allele number was added by an OMP curator following the pattern seen in papers from the Simon lab of naming alleles based on strain numbers. [[User:Siegele|Siegele]] ([[User talk:Siegele|talk]]) 13:44, 18 July 2016 (CDT)
  
 
==Phenotype Annotation==
 
==Phenotype Annotation==
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!|Annotation ID!!not!!OMP ID!!OMP term name!!Relative phenotype information!!Experimental condition!!ECO ID!!ECO term name!!Reference!!Annotation Extension!!Notes
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!|Annotation ID!!Qualifier!!OMP ID!!OMP term name!!Relative phenotype information!!Experimental condition!!ECO ID!!ECO term name!!Reference!!Annotation Extension!!Notes
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OMP_AN:692
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OMP:0007000
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abolished motility
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Relative to: OMP_AN:691
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<hr>Genotype differences:<br>
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+flgE2008(Am)
 +
<hr>Condition differences:<br>
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no differences
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|
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*medium:Tryptone motility agar
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*medium:thymidine (0.1 g per liter)
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ECO:0007192
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motility assay evidence used in manual assertion
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PMID:338588
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OMP_AN:694
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OMP:0007245
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abolished sensitivity to bacteriophage
 +
|
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Relative to: OMP_AN:693
 +
 
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<hr>Genotype differences:<br>
 +
+flgE2008(Am)
 +
<hr>Condition differences:<br>
 +
no differences
 +
|
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*temperature:37C
 +
*medium:Tryptone agar
 +
*medium:thymidine (0.1 g per liter)
 +
|
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ECO:0007040
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plaque assay evidence used in manual assertion
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PMID:338588
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Bacteriophage Chi [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=1541887 Taxonomy ID: 1541887]
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Latest revision as of 18:03, 14 September 2018

Strain Summary

Strain Name

YK2008

Synonyms
Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

F- lambda- e14-(?) Rac-0(?) uvrC34 hisG4(Oc) rfbC1 mgl-51 rpsL31(strR) kdgK51 xylA5 mtl-1 argE3(Oc) thiE1 thyA galU51 pyrC46 nalA flgE2008(Am)

Strain Reference

PMID:338588[1]

Strain availability
Ancestry

parent:OMP ST:927 ! Escherichia coli K-12 YK102

Annotated phenotypes


edit table


Notes

YK2008 was isolated by selecting for derivatives of YK102 that are resistant to phage Chi and then screening for loss of motility. Note that in the paper[1], the flgE gene is referred to by the older name, flaK.

For the flgE2008(Am) mutation, the allele number was added by an OMP curator following the pattern seen in papers from the Simon lab of naming alleles based on strain numbers. Siegele (talk) 13:44, 18 July 2016 (CDT)

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:692

OMP:0007000

abolished motility

Relative to: OMP_AN:691

Genotype differences:

+flgE2008(Am)

Condition differences:

no differences

  • medium:Tryptone motility agar
  • medium:thymidine (0.1 g per liter)
 ECO:0007192

motility assay evidence used in manual assertion

PMID:338588[1]

OMP_AN:694

OMP:0007245

abolished sensitivity to bacteriophage

Relative to: OMP_AN:693

Genotype differences:

+flgE2008(Am)

Condition differences:

no differences

  • temperature:37C
  • medium:Tryptone agar
  • medium:thymidine (0.1 g per liter)
 ECO:0007040

plaque assay evidence used in manual assertion

PMID:338588[1]

Bacteriophage Chi Taxonomy ID: 1541887


edit table


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ 1.0 1.1 1.2 1.3 Komeda, Y et al. (1978) Identification of the structural gene for the hook subunit protein of Escherichia coli flagella. J. Bacteriol. 133 364-71 PubMed OMPwiki page
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