Difference between revisions of "OMP ST:928 ! Escherichia coli K-12 YK2008"

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!|Annotation ID!!not!!OMP ID!!OMP term name!!Relative phenotype information!!Experimental condition!!ECO ID!!ECO term name!!Reference!!Annotation Extension!!Notes
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!|Annotation ID!!Qualifier!!OMP ID!!OMP term name!!Relative phenotype information!!Experimental condition!!ECO ID!!ECO term name!!Reference!!Annotation Extension!!Notes
 
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OMP:0007000
 
OMP:0007000
 
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abolished cell motility
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abolished motility
 
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Relative to: OMP_AN:691
 
Relative to: OMP_AN:691
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+flgE2008(Am)
 
+flgE2008(Am)
 
<hr>Condition differences:<br>
 
<hr>Condition differences:<br>
medium:Motility agarMotility Agar
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other:indicator strain-[[OMP ST?
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medium:Tryptone agarTryptone motility agar
 
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medium:Motility agar
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*other:indicator strain-[[OMP ST:927 ! Escherichia coli K-12 YK102|E. coli K-12 YK102]]
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*medium:Tryptone agar
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*medium:thymidine (0.1 g per liter)
 
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ECO:0007040
 
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plaque assay evidence used in manual assertion
 
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PMID:338588
 
PMID:338588
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Revision as of 17:53, 14 September 2018

Strain Summary

Strain Name

YK2008

Synonyms
Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

F- lambda- e14-(?) Rac-0(?) uvrC34 hisG4(Oc) rfbC1 mgl-51 rpsL31(strR) kdgK51 xylA5 mtl-1 argE3(Oc) thiE1 thyA galU51 pyrC46 nalA flgE2008(Am)

Strain Reference

PMID:338588[1]

Strain availability
Ancestry

parent:OMP ST:927 ! Escherichia coli K-12 YK102

Annotated phenotypes



Notes

YK2008 was isolated by selecting for derivatives of YK102 that are resistant to phage Chi and then screening for loss of motility. Note that in the paper[1], the flgE gene is referred to by the older name, flaK.

For the flgE2008(Am) mutation, the allele number was added based on the pattern seen in papers from the Simon lab of naming alleles based on strain numbers. Siegele (talk) 13:44, 18 July 2016 (CDT)

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:692

OMP:0007000

abolished motility

Relative to: OMP_AN:691


Genotype differences:

+flgE2008(Am)


Condition differences:

other:indicator strain-[[OMP ST? medium:Tryptone agarTryptone motility agar

  • other:indicator strain-E. coli K-12 YK102
  • medium:Tryptone agar
  • medium:thymidine (0.1 g per liter)
ECO:0007040

plaque assay evidence used in manual assertion

PMID:338588[1]

OMP_AN:694

OMP:0007245

abolished sensitivity to bacteriophage

Relative to: OMP_AN:693


Genotype differences:

+flgE2008(Am)


Condition differences:

no differences

PMID:338588[1]

Taxonomy ID: 1541887 Phage Chi



Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. 1.0 1.1 1.2 1.3 Komeda, Y et al. (1978) Identification of the structural gene for the hook subunit protein of Escherichia coli flagella. J. Bacteriol. 133 364-71 PubMed OMPwiki page