OMP ST:773 ! Escherichia coli K-12 AW518

From OMPwiki

Strain Summary

Strain Name

AW518

Synonyms
Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

Lambda- araC14 fhuA31 galK2(Oc) galT22 glnX44(AS) hisG4(Oc) lacY1 leuB6(Am) mgl-51 mtl-1 rfbC1 rpsL136(StrR) thiE1 thr-1 tsr-1 tsx-78 xylA5

Strain Reference

PMID:4884810[1]

Strain availability
Ancestry

parent:OMP ST:772 ! Escherichia coli K-12 AW405

Annotated phenotypes


Notes

AW405 is a derivative of AW330. The his-4 allele was introduced from strain AT1372[2] by transduction with P1, followed by penicillin selection.[2]

The tsr mutation in AW518 was named tsr-1 in Hedblom and Adler[3].

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:47

OMP:0000005

presence of cell motility

Relative to:


Genotype differences:

Nothing to compare


Condition differences:

Nothing to compare

  • temperature:35C
  • medium:semi-solid tryptone agar
ECO:0000181

in vitro assay evidence

PMID:4916925[4]

OMP_AN:48

OMP:0007041

decreased positive chemotaxis

Relative to: OMP_AN:45


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:35C
  • medium:semi-solid tryptone agar
ECO:0000181

in vitro assay evidence

PMID:4916925[4]

OMP_AN:49

OMP:0007041

decreased positive chemotaxis

Relative to: OMP_AN:46


Genotype differences:

+tsr-1


Condition differences:

medium:buffer + serine (CHEBIbuffer;serine (CHEBI

medium:buffer + serine (CHEBI:17115)

ECO:0000181

in vitro assay evidence

PMID:4916925[4]

OMP_AN:341

OMP:0007005

decreased negative chemotaxis

Relative to: OMP_AN:403


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:acetate
  • medium:chemotaxis medium
ECO:0007187

tumble frequency assay evidence used in manual assertion

PMID:333433[5]

CHEBI:30089 acetate

OMP_AN:342

OMP:0007005

decreased negative chemotaxis

Relative to: OMP_AN:404


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:benzoate
  • medium:chemotaxis medium
ECO:0005608

tumble frequency assay evidence

PMID:333433[5]

CHEBI:16150 benzoate

OMP_AN:343

OMP:0007005

decreased negative chemotaxis

Relative to: OMP_AN:405


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:chemotaxis medium
  • medium:indole
ECO:0005608

tumble frequency assay evidence

PMID:333433[5]

CHEBI:35581 indole

OMP_AN:344

OMP:0007005

decreased negative chemotaxis

Relative to: OMP_AN:406


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:chemotaxis medium
  • medium:leucine
ECO:0005608

tumble frequency assay evidence

PMID:333433[5]

CHEBI:15603 leucine

OMP_AN:345

OMP:0007053

increased positive chemotaxis

Relative to: OMP_AN:105


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:aspartate
  • medium:chemotaxis medium
ECO:0005608

tumble frequency assay evidence

PMID:333433[5]

CHEBI:17053 aspartate

OMP_AN:346

OMP:0007053

increased positive chemotaxis

Relative to: OMP_AN:102


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:chemotaxis medium
  • medium:maltose
ECO:0005608

tumble frequency assay evidence

PMID:333433[5]

CHEBI:17306 maltose

OMP_AN:347

OMP:0007053

increased positive chemotaxis

Relative to: OMP_AN:401


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:chemotaxis medium
  • medium:galactose
ECO:0005609

capillary assay evidence

PMID:333433[5]

CHEBI:28260 galactose

OMP_AN:348

OMP:0007053

increased positive chemotaxis

Relative to: OMP_AN:402


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:chemotaxis medium
  • medium:ribose
ECO:0005609

capillary assay evidence

PMID:333433[5]

CHEBI:33942 ribose

OMP_AN:349

OMP:0007054

increased negative chemotaxis

Relative to: OMP_AN:407


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:Ni(II)
  • medium:chemotaxis medium
ECO:0005608

tumble frequency assay evidence

PMID:333433[5]

OMP_AN:350

OMP:0007054

increased negative chemotaxis

Relative to: OMP_AN:408


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:Co(II)
  • medium:chemotaxis medium
ECO:0005608

tumble frequency assay evidence

PMID:333433[5]

CHEBI:48828 Co(II)

OMP_AN:422

OMP:0007041

decreased positive chemotaxis

Relative to: OMP_AN:421


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:chemotaxis medium
  • medium:serine
ECO:0007187

tumble frequency assay evidence used in manual assertion

PMID:333433[5]

CHEBI:17115 serine

OMP_AN:494

OMP:0007041

decreased positive chemotaxis

Relative to: OMP_AN:492


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:alanine
  • medium:chemotaxis medium
ECO:0005609

capillary assay evidence

PMID:4562400[6]

CHEBI:16977 alanine

Threshold for response increased

OMP_AN:495

OMP:0007041

decreased positive chemotaxis

Relative to: OMP_AN:488


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:asparagine
  • medium:chemotaxis medium
ECO:0005609

capillary assay evidence

PMID:4562400[6]

CEHBI:17196 asparagine

Threshold for response increased

OMP_AN:496

OMP:0007041

decreased positive chemotaxis

Relative to: OMP_AN:489


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:chemotaxis medium
  • medium:cysteine
ECO:0005609

capillary assay evidence

PMID:4562400[6]

CHEBI:17561 cysteine

Threshold for response increased

OMP_AN:497

OMP:0007041

decreased positive chemotaxis

Relative to: OMP_AN:491


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:chemotaxis medium
  • medium:glycine
ECO:0005609

capillary assay evidence

PMID:4562400[6]

Threshold for response increased

OMP_AN:498

OMP:0007041

decreased positive chemotaxis

Relative to: OMP_AN:486


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:chemotaxis medium
  • medium:serine
ECO:0005609

capillary assay evidence

PMID:4562400[6]

CHEBI:17715 serine

Threshold for response increased

OMP_AN:499

OMP:0007041

decreased positive chemotaxis

Relative to: OMP_AN:487


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:chemotaxis medium
  • medium:threonine
ECO:0005609

capillary assay evidence

PMID:4562400[6]

CHEBI:16857 threonine

Threshold for response incresased

OMP_AN:500

OMP:0007053

increased positive chemotaxis

Relative to: OMP_AN:493


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:chemotaxis medium
  • medium:methionine
ECO:0005609

capillary assay evidence

PMID:4562400[6]

CHEBI:16643 methionine

OMP_AN:562

OMP:0007041

decreased positive chemotaxis

Relative to: OMP_AN:543


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:L-serine
  • medium:chemotaxis medium
ECO:0005609

capillary assay evidence

PMID:6350273[7]

CHEBI:17115 L-serine

Synthetic amino acid

OMP_AN:563

OMP:0007041

decreased positive chemotaxis

Relative to: OMP_AN:547


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:chemotaxis medium
  • medium:glycine
ECO:0005609

capillary assay evidence

PMID:6350273[7]

CHEBI:15428 glycine

Synthetic amino acid

OMP_AN:564

OMP:0007041

decreased positive chemotaxis

Relative to: OMP_AN:550


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:L-threonine
  • medium:chemotaxis medium
ECO:0005609

capillary assay evidence

PMID:6350273[7]

CHEBI:16857 L-threonine

Synthetic amino acid

OMP_AN:565

OMP:0007041

decreased positive chemotaxis

Relative to: OMP_AN:551


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:L-proline
  • medium:chemotaxis medium
ECO:0005609

capillary assay evidence

PMID:6350273[7]

CHEBI:17203 L-proline

Synthetic amino acid

OMP_AN:566

OMP:0007005

decreased negative chemotaxis

Relative to: OMP_AN:553


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:L-histidine
  • medium:chemotaxis medium
ECO:0005609

capillary assay evidence

PMID:6350273[7]

CHEBI:15971 L-histidine

Synthetic amino acid

OMP_AN:567

OMP:0007005

decreased negative chemotaxis

Relative to: OMP_AN:554


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:L-isoleucine
  • medium:chemotaxis medium
ECO:0005609

capillary assay evidence

PMID:6350273[7]

CHEBI:17191 L-isoleucine

Synthetic amino acid

OMP_AN:568

OMP:0007005

decreased negative chemotaxis

Relative to: OMP_AN:555


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:L-leucine
  • medium:chemotaxis medium
ECO:0005609

capillary assay evidence

PMID:6350273[7]

CHEBI:15603 L-leucine

Synthetic amino acid

OMP_AN:569

OMP:0007005

decreased negative chemotaxis

Relative to: OMP_AN:557


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:L-tryptophan
  • medium:chemotaxis medium
ECO:0005609

capillary assay evidence

PMID:6350273[7]

CHEBI:16828 L-tryptophan

Synthetic amino acid

OMP_AN:585

OMP:0000054

absence of positive chemotaxis

Relative to: OMP_AN:573


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:LiCl
  • medium:chemotaxis medium
ECO:0005609

capillary assay evidence

PMID:2682648[8]

CHEBI:48607 LiCl

Salt chemotaxis

OMP_AN:586

OMP:0000054

absence of positive chemotaxis

Relative to: OMP_AN:578


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:MgCl2
  • medium:chemotaxis medium
ECO:0005609

capillary assay evidence

PMID:2682648[8]

CHEBI:6636 MgCl2

Salt chemotaxis

OMP_AN:587

OMP:0007041

decreased positive chemotaxis

Relative to: OMP_AN:572


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:NH4Cl
  • medium:chemotaxis medium
ECO:0005609

capillary assay evidence

PMID:2682648[8]

CHEBI:31206 NH4Cl

Salt chemotaxis

OMP_AN:588

OMP:0007041

decreased positive chemotaxis

Relative to: OMP_AN:576


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:RbCl
  • medium:chemotaxis medium
ECO:0005609

capillary assay evidence

PMID:2682648[8]

Salt chemotaxis

OMP_AN:589

OMP:0007041

decreased positive chemotaxis

Relative to: OMP_AN:577


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:CsCl
  • medium:chemotaxis medium
ECO:0005609

capillary assay evidence

PMID:2682648[8]

Salt chemotaxis

OMP_AN:590

OMP:0007041

decreased positive chemotaxis

Relative to: OMP_AN:582


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:NaF
  • medium:chemotaxis medium
ECO:0005609

capillary assay evidence

PMID:2682648[8]

CHEBI:28741 NaF

Salt chemotaxis

OMP_AN:591

OMP:0007041

decreased positive chemotaxis

Relative to: OMP_AN:574


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:NaCl
  • medium:chemotaxis medium
ECO:0005609

capillary assay evidence

PMID:2682648[8]

CHEBI:26710 NaCl

Salt chemotaxis

OMP_AN:592

OMP:0007041

decreased positive chemotaxis

Relative to: OMP_AN:584


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:NaBr
  • medium:chemotaxis medium
ECO:0005609

capillary assay evidence

PMID:2682648[8]

CHEBI:63004 NaBr

Salt chemotaxis

OMP_AN:593

OMP:0007041

decreased positive chemotaxis

Relative to: OMP_AN:583


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:NaI
  • medium:chemotaxis medium
ECO:0005609

capillary assay evidence

PMID:2682648[8]

CHEBI:33167 NaI

Salt chemotaxis

OMP_AN:594

OMP:0007041

decreased positive chemotaxis

Relative to: OMP_AN:575


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:KCl
  • medium:chemotaxis medium
ECO:0005609

capillary assay evidence

PMID:2682648[8]

CHEBI:32588 KCl

Salt chemotaxis

OMP_AN:595

OMP:0007041

decreased positive chemotaxis

Relative to: OMP_AN:580


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:BaCl2
  • medium:chemotaxis medium
ECO:0005609

capillary assay evidence

PMID:2682648[8]

CHEBI:63317 BaCl2

Salt chemotaxis

OMP_AN:596

OMP:0007041

decreased positive chemotaxis

Relative to: OMP_AN:581


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:CaCl2
  • medium:chemotaxis medium
ECO:0005609

capillary assay evidence

PMID:2682648[8]

CHEBI:3312 CaCl2

Salt chemotaxis

OMP_AN:589

OMP:0007041

decreased positive chemotaxis

Relative to: OMP_AN:577


Genotype differences:

+tsr-1


Condition differences:

no differences

  • temperature:30C
  • medium:CsCl
  • medium:chemotaxis medium
ECO:0005609

capillary assay evidence

PMID:2682648[8]

Salt chemotaxis



Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. Armstrong, JB & Adler, J (1969) Location of genes for motility and chemotaxis on the Escherichia coli genetic map. J. Bacteriol. 97 156-61 PubMed OMPwiki page
  2. Armstrong, JB & Adler, J (1967) Genetics of motility in Escherichia coli: complementation of paralysed mutants. Genetics 56 363-73 PubMed OMPwiki page
  3. Hiatt, RA et al. (1978) Limitations of the intradermal test for schistosomiasis mansoni: experience from epidemiologic studies in a Puerto Rican community. Am. J. Trop. Med. Hyg. 27 535-41 PubMed OMPwiki page
  4. 4.0 4.1 4.2 Hazelbauer, GL et al. (1969) Escherichia coli mutants defective in chemotaxis toward specific chemicals. Proc. Natl. Acad. Sci. U.S.A. 64 1300-7 PubMed OMPwiki page
  5. 5.00 5.01 5.02 5.03 5.04 5.05 5.06 5.07 5.08 5.09 5.10 Springer, MS et al. (1977) Sensory transduction in Escherichia coli: two complementary pathways of information processing that involve methylated proteins. Proc. Natl. Acad. Sci. U.S.A. 74 3312-6 PubMed OMPwiki page
  6. 6.0 6.1 6.2 6.3 6.4 6.5 6.6 Mesibov, R & Adler, J (1972) Chemotaxis toward amino acids in Escherichia coli. J. Bacteriol. 112 315-26 PubMed OMPwiki page
  7. 7.0 7.1 7.2 7.3 7.4 7.5 7.6 7.7 Hedblom, ML & Adler, J (1983) Chemotactic response of Escherichia coli to chemically synthesized amino acids. J. Bacteriol. 155 1463-6 PubMed OMPwiki page
  8. 8.00 8.01 8.02 8.03 8.04 8.05 8.06 8.07 8.08 8.09 8.10 8.11 8.12 Qi, YL & Adler, J (1989) Salt taxis in Escherichia coli bacteria and its lack in mutants. Proc. Natl. Acad. Sci. U.S.A. 86 8358-62 PubMed OMPwiki page